A5043218202- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant and animal studies
- Plant Molecular Biology Research
- Agronomic Practices and Intercropping Systems
- Weed Control and Herbicide Applications
- Plant tissue culture and regeneration
- Plant Virus Research Studies
- Allelopathy and phytotoxic interactions
- Plant and Fungal Species Descriptions
- Genetic and Environmental Crop Studies
- Plant Diversity and Evolution
- Photosynthetic Processes and Mechanisms
- Plant Genetic and Mutation Studies
- Pesticide and Herbicide Environmental Studies
- Seed Germination and Physiology
- Soybean genetics and cultivation
- Ecology and Vegetation Dynamics Studies
- Nematode management and characterization studies
- Plant biochemistry and biosynthesis
- Botanical Research and Chemistry
- Genetic Mapping and Diversity in Plants and Animals
- Plant Reproductive Biology
- Insect symbiosis and bacterial influences
- Insect and Arachnid Ecology and Behavior
Virginia Tech
2015-2025
Healthcare Quality Improvement Partnership
2024
Office for Health Improvement and Disparities
2024
University of Algiers 3
2021
University of New Caledonia
2019
Marymount University
2018
GTx (United States)
1998
Purdue University West Lafayette
1997
Abstract The discipline of weed science is at a critical juncture. Decades efficient chemical control have led to rise in the number herbicide-resistant populations, with few new herbicides unique modes action counter this trend and often no economical alternatives large-acreage crops. At same time, world population swelling, necessitating increased food production feed an anticipated 9 billion people by year 2050. Here, we consider these challenges along emerging trends technology...
Obligate parasitic plants in the Orobanchaceae germinate after sensing plant hormones, strigolactones, exuded from host roots. In Arabidopsis thaliana, α/β-hydrolase D14 acts as a strigolactone receptor that controls shoot branching, whereas its ancestral paralog, KAI2, mediates karrikin-specific germination responses. We observed but not D14, is present at higher copy numbers species than nonparasitic relatives. KAI2 paralogs parasites are distributed into three phylogenetic clades. The...
Movement of RNAs between cells a single plant is well documented, but cross-species RNA transfer largely unexplored. Cuscuta pentagona (dodder) parasitic that forms symplastic connections with its hosts and takes up host messenger (mRNAs). We sequenced transcriptomes growing on Arabidopsis tomato to characterize mRNA species found mRNAs move in high numbers bidirectional manner. The mobile transcripts represented thousands different genes, nearly half the expressed transcriptome was...
Abstract The genome of Arabidopsis (Arabidopsis thaliana) contains five sequences with high similarity to FLAVONOL SYNTHASE1 (AtFLS1), a previously characterized flavonol synthase gene that plays central role in flavonoid metabolism. This apparent redundancy suggests the possibility uses multiple isoforms FLS different substrate specificities mediate production flavonols, quercetin and kaempferol, tissue-specific inducible manner. However, biochemical genetic analysis six AtFLS indicates...
The origin of novel traits is recognized as an important process underlying many major evolutionary radiations. We studied the genetic basis for evolution haustoria, feeding organs parasitic flowering plants, using comparative transcriptome sequencing in three species Orobanchaceae. Around 180 genes are upregulated during haustorial development following host attachment at least two species, and these enriched proteases, cell wall modifying enzymes, extracellular secretion proteins....
The Parasitic Plant Genome Project has sequenced transcripts from three parasitic species and a nonparasitic relative in the Orobanchaceae with goal of understanding genetic changes associated parasitism. studied span trophic spectrum free-living nonparasite to obligate holoparasite. used were Triphysaria versicolor , photosynthetically competent that opportunistically parasitizes roots neighboring plants; Striga hermonthica hemiparasite an need for host; Orobanche aegyptiaca holoparasite...
Horizontal gene transfer (HGT) is the of genetic material across species boundaries and has been a driving force in prokaryotic evolution. HGT involving eukaryotes appears to be much less frequent, functional implications are poorly understood. We test hypothesis that parasitic plants, because their intimate feeding contacts with host plant tissues, especially prone horizontal acquisition. sought evidence HGTs transcriptomes three members Orobanchaceae, family containing spanning full...
An intriguing new paradigm in plant biology is that systemically mobile mRNAs play a role coordinating development. In this process, specific are loaded into the phloem transport stream for translocation to distant tissues, where they may impact on developmental processes. However, despite its potential significance growth regulation, mRNA trafficking remains poorly understood and challenging study. Here, we show phloem-mobile can also traffic between widely divergent species from host...
Experiments were conducted to evaluate a biotype of smooth pigweed that had survived applications sulfonylurea (SU) and imidazolinone (IMI) herbicides in single season. The source field history repeated acetolactate synthase (ALS)-inhibiting herbicide use over several years. Whole-plant response experiments evaluated the resistant (R11) an ALS-inhibitor susceptible (S) from SU, IMI, pyrimidinylthiobenzoate (PTB), triazolopyrimidine sulfonanilide (TP) chemical families. R11 exhibited 60-...
Abstract Background Orobanchaceae is the only plant family with members representing full range of parasitic lifestyles plus a free-living lineage sister to all lineages, Lindenbergia . A generalist member this family, and an important model, Triphysaria versicolor regularly feeds upon wide host plants. Here, we compare de novo assembled transcriptomes generated from laser micro-dissected tissues at host-parasite interface uncover details largely uncharacterized interaction between plants...
The genetic basis of weedy and invasive traits their evolution remain poorly understood, but genomic approaches offer tremendous promise for elucidating these important features weed biology. However, the tools resources available research are currently meager compared with those many crops. Because methodologies becoming increasingly accessible less expensive, time is ripe scientists to incorporate methods into programs. One example next-generation sequencing technology, which has advantage...
Parasitization by Orobanche is a complex process, one that mediated host-derived chemical signals control parasite seed germination and haustorium initiation ultimately results in the union of two plant species. Experiments were conducted to characterize parasitization model Arabidopsis thaliana begin explore role host flavonoid metabolism interaction. stimulated allowed tubercle development O. aegyptiaca ramosa but did not significantly stimulate seeds crenata, minor, or cernua. However, if...
A number of plant species have adapted to parasitize other plants, and some parasitic pose severe constraints major crops. The role strigolactones metabolites present in host root exudates as germination stimulants for weedy weed seeds has been known the last 40 years. Recently, ecological developmental roles clarified by discovery that they are a new class hormone controls shoot branching serve recognition signals mycorrhizal fungi. Parasitic plants also recognize these chemicals use them...
Summary The cross‐species movement of mRNA from hosts to the parasitic plant Cuscuta pentagona has been reported previously, but not characterized quantitatively or with attention uptake patterns and fate specific s. Real‐time PCR RNA ‐Seq approaches were used identify characterize mobile transcripts tomato Arabidopsis into C. . Tomato Gibberellic Acid Insensitive ( Sl GAI ) Cathepsin D Proteinase Inhibitor PI differed significantly in rate parasite, then distributed over length parasite...
Abstract Background Parasitic plants, represented by several thousand species of angiosperms, use modified structures known as haustoria to tap into photosynthetic host plants and extract nutrients water. As a result their direct plant-plant connections with plant, parasitic have special opportunities for horizontal gene transfer, the nonsexual transmission genetic material across boundaries. There is increasing evidence that served recipients donors transfer (HGT), but long-term impacts...