A5041366943- Diphtheria, Corynebacterium, and Tetanus
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
- Streptococcal Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Identification and Quantification in Food
- Probiotics and Fermented Foods
- Infective Endocarditis Diagnosis and Management
- Microbial infections and disease research
- Biosensors and Analytical Detection
- Neonatal and Maternal Infections
- Genomics and Phylogenetic Studies
- Meat and Animal Product Quality
- Veterinary medicine and infectious diseases
- Molecular Biology Techniques and Applications
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Microbial Metabolism and Applications
- Botulinum Toxin and Related Neurological Disorders
- Ginseng Biological Effects and Applications
- Infectious Diseases and Mycology
- Bacillus and Francisella bacterial research
- Animal health and immunology
University of Veterinary Medicine Hannover, Foundation
2016-2025
Agriculture and Agri-Food Canada
2021-2022
Ottawa Research and Development Centre
2021-2022
University of Veterinary Medicine Vienna
2014-2015
Justus-Liebig-Universität Gießen
2003-2012
Universitas Gadjah Mada
2004
University of Cincinnati
2003
University of Cincinnati Medical Center
2003
Giessen School of Theology
2002
The explosive increase since the beginning of 1990s in number publications reporting PCR-based methods for detection or molecular typing food-borne pathogens has attracted attention end user laboratories. However, well-recognized difficulties reproducing published tests due to variation performance PCR thermal cyclers (7) and efficiencies different DNA polymerases presence inhibitors sample matrix have hampered implementation This particularly applies laboratories with quality assurance...
Campylobacteriosis is the most frequent food-borne human enteritis. The major source for infection with Campylobacter spp. broiler meat. Risk assessments consider reduction of in primary production to be beneficial health. aim this study was test efficacy a bacteriophage application under commercial conditions which had proved effective previous noncommercial studies controlled experimental conditions. A phage cocktail tested on three farms each control and an group. Colonization confirmed...
Animal species identification is one of the primary duties official food control. Since ostrich meat difficult to be differentiated macroscopically from beef, therefore new analytical methods are needed. To enforce labeling regulations for authentication meat, it might importance develop and evaluate a rapid reliable assay. In present study, loop-mediated isothermal amplification (LAMP) assay based on cytochrome b gene mitochondrial DNA Struthio camelus was developed. The LAMP used in...
With regards to the frequently reported findings of spoilage bacteria and pathogens in various foods there is a need explore new ways control hazards food production improve consumer safety. Fermented sausages from traditional industrial Germany were screened for lactic acid with antibacterial effects towards important foodborne (Escherichia coli DSM 1103, Listeria innocua 20649, monocytogenes 19094, Pseudomonas aeruginosa 939, Staphylococcus aureus 799 Salmonella Typhimurium 19587). The...
In the present study, a robust TaqMan real-time PCR amplifying F57 and ISMav2 sequences of Mycobacterium avium subsp. paratuberculosis from bovine fecal samples was developed validated. The validation based on recommendations International Organization for Standardization protocols methods. For specificity testing, 205 bacterial strains were selected, including 105 M. bovine, ovine, human origin 100 non-M. strains. Diagnostic quality assurance obtained by use an internal amplification...
Entomophagy is an ancient and actually African tradition that has been receiving renewed attention since edible insects have identified as one of the solutions to improve global nutrition. As any other foodstuff, should be regulated by government ensure product quality consumer safety. The goal present paper was assess current legal status in Africa. For that, corresponding authorities were contacted along with extensive online search, relying mostly on FAOLEX database. Except for Botswana,...
In the present study 130 S. uberis strains and one parauberis strain isolated from bovine milk samples of 58 different farms various locations in Hesse, Germany, as well two reference each species were comparatively investigated for cultural, biochemical, serological molecular properties. All produced enzyme beta-D-glucuronidase, while negative. The 16S rRNA genes amplified by polymerase chain reaction subsequently digested with restriction enzymes RsaI AvaII yielding species-specific...
For almost a decade, edible insects have become promoted on wider basis as one way to combat world hunger and malnourishment, although attempts do so longer history. Contemporary researchers consumers, particularly those without an entomophagous background, been rising safety sustainability concerns. The present contribution seeks substantiated answer the question posed above. possible consists of different factors that taken into consideration. First, species its life cycle. It is mandatory...
Background and Aim: Ovine mastitis, particularly subclinical mastitis caused by Staphylococcus aureus, poses significant economic health challenges in livestock management. This study aimed to investigate the molecular characteristics antimicrobial-resistance gene profiles of S. aureus isolated from northern Jordan assess zoonotic potential these isolates. Materials Methods: A total 283 milk samples ovines 24 nasal swabs animal handlers across three Jordanian governorates were analyzed....
Clostridium (C.) perfringens is the causative agent of several diseases in animals and humans, including histotoxic enteric infections. To gain more insight into occurrence its different toxin-genotypes dairy herds, those toxin genes previously associated with cattle or 662 isolates cultivated from feces, rumen content feed collected 139 farms were characterized by PCR (detecting cpa, cpb, iap, etx, cpe, both allelic variants cpb2). Isolates feces assigned to type A (cpa positive, n = 442) D...
In this study, changes in hepatitis E virus (HEV) contamination the production of liver sausage from naturally contaminated pork were investigated. Furthermore, potential effectiveness individual parameters reducing viral loads was measured. When processing moderately (initial Cq-value 29), HEV RNA persisted finished sausages, even after heating for 90 min at 75 °C. A matrix-specific standard curve created using a spiking experiment to accurately quantify particularly challenging matrix like...
Streptococcus uberis and parauberis reference strains isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, 23S 16S-23S intergenic spacer region both species. All three primer pairs allowed an identification 67 as S. 4 parauberis.
ABSTRACT Bacteriological investigations of seals the German North and Baltic seas resulted in isolation bacteria genus Streptococcus belonging to Lancefield's serological groups C, F, L. According biochemical, serological, 16S ribosomal DNA analysis, group C F streptococci were identified as phocae . The L could be classified dysgalactiae subsp.
The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All showed the cultural biochemical properties typical A. were susceptible most antibiotics tested. species identification could be confirmed by amplification sequencing 16S rRNA gene 16S-23S intergenic spacer region PCR species-specific parts encoding phospholipase D in haemolyticum. Use latter possibly improve future this...
The present study was designed to identify phenotypically and genotypically two Arcanobacterium (A.) pyogenes strains isolated by post mortem examinations of a bearded dragon gecko. A. showed the typical biochemical properties displayed CAMP-like synergistic hemolytic activities with various indicator strains. species identity could be confirmed amplification sequencing 16S rDNA gene and, as novel target gene, beta subunit RNA polymerase encoding rpoB, both reference representing nine genus...
A polyphasic taxonomic study was performed on two previously unidentified Arcanobacterium-like Gram-positive strains isolated from harbour seals. Comparative 16S rRNA gene sequencing showed that both bacteria belonged to the genus Arcanobacterium and were most closely related haemolyticum CIP 103370(T) (98.4% sequence similarity), A. canis P6775(T) (97.4%), phocae DSM 10002(T) pluranimalium M430/94/2(T) (95.7%) hippocoleae CCUG 44697(T) (95.5%). The presence of major menaquinone MK-9(H4)...
A polyphasic taxonomic study was performed on an unidentified Arcanobacterium-like Gram-stain-positive bacterium isolated from otitis externa of a dog. Comparative 16S rRNA gene sequencing showed that the belonged to genus Arcanobacterium and most closely related type strains haemolyticum (97.2 %), hippocoleae (96.5 %) phocae (96.4 %). The presence major menaquinone MK-9(H(4)) supported affiliation this strain Arcanobacterium. polar lipid profile contained lipids phosphatidylcholine,...
The present study was designed to investigate the potential of Fourier transform infrared (FT-IR) spectroscopy identify Trueperella (T.) pyogenes isolated from bovine clinical mastitis. FT-IR applied 57 isolates obtained 55 cows in a period 2009 2012. Prior these were identified by phenotypic and genotypic properties, also including determination seven virulence factor encoding genes. analysis revealed reliable identification all as T. clear separation this species other genus...