A5101617641- Helminth infection and control
- Parasites and Host Interactions
- Parasite Biology and Host Interactions
- Cell Adhesion Molecules Research
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Coccidia and coccidiosis research
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Complement system in diseases
- Reproductive Physiology in Livestock
- Phytochemical compounds biological activities
- Synthesis and biological activity
- Parasitic Diseases Research and Treatment
- Animal Nutrition and Physiology
- Platelet Disorders and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Cellular Mechanics and Interactions
- Blood Coagulation and Thrombosis Mechanisms
- Protease and Inhibitor Mechanisms
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Insect Utilization and Effects
Indian Veterinary Research Institute
2010-2022
Division of Chemistry
2008
University of North Carolina at Chapel Hill
1993-1997
Savitribai Phule Pune University
1967-1995
Duke University
1993-1994
Duke University Hospital
1993
Duke Medical Center
1993
Indian Institute of Science Bangalore
1987
We have produced a set of bacterial expression proteins corresponding to 10 segments tenascin and two fibronectin tested them for heparin binding cell adhesion. used polymerase chain reaction cloning terminate the precisely at domain boundaries. Heparin activity was mapped different segments: one comprising fourth fifth type III domains, TNfbg, fibrinogen-like terminal knob. but none other tanascin segments, also supported adhesion primary rat embryo skin fibroblasts. The fibroblasts did not...
ABSTRACT We have found that endothelial cells adhere much more strongly than fibroblasts to domains of tenascin and fibronectin. Endothelial adhered weakly, without spreading, bacterial expression proteins corresponding the tenth fibronectin type III (FN-III) domain fibronectin, which contains RGD. A larger protein, containing this three amino-terminal ‘synergy’ gave strong adhesion spreading. Two widely separated adhesion. The third FN-III domain, TNfn3, an RGD sequence in human chicken...
Abstract Crystals of a fragment human fibronectin encompassing the 7th through RGD‐containing 10th type III repeats (FN7–10) have been produced with protein expressed in E. coli. The crystals are monoclinic one molecule asymmetric unit and diffract to beyond 2.0 Å Bragg spacings. A mutant FN7–10 was which three methionines, addition single native methionine already present, introduced by site‐directed mutagenesis. Diffraction‐quality this grown replaced selenomethionine. introduction...
Haemonchus contortus is an economically important gastrointestinal parasite that infects primarily sheep and goats. To survive inside the host, must overcome host immune response. In this study, we have identified characterized a complement-C3-binding protein (H.c-C3BP) from employing biochemical molecular biology tools. Initially, truncated form of was isolated excretory-secretory products using C3-Sepharose column facilitated its identification by mass spectroscopy. Subsequently, parent...
SUMMARY Haemonchus contortus is an economically important gastric parasite infecting sheep and goats. The survives the host immune attack by releasing protective molecules. In present study, a 55 kDa secretory glycoprotein (gp55) was identified that inhibited neutrophils as judged reduced H 2 O production these cells. binding of gp55 to confirmed flow cytometry. This mediated cellular CD11b/CD18 integrin. also bound goat monocytes lymphocytes monocyte function. Using light fluorescence...
Haemonchus contortus, an economically important blood-sucking parasite of sheep and goats, survives the harsh host gut environment by secreting a number proteins referred as excretory/secretory (ES) products. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), glycolytic enzyme, is one components H. contortus ES The enzyme binds to complement C3 inhibits its activity. In this study, C3-binding activity GAPDH was mapped N-terminal part generating defined recombinant fragments protein. fragment...
Angiotensin converting enzyme (ACE) activity has been reported in testis and epididymis of seven different animal species. Among all the species, mouse showed highest followed by rat epididymis. The lowest was detected buffalo rabbit Most testicular found concentrated 107,00 X g sediment while epididymal equally distributed between supernatant. ACE levels regions analyzed. gradient increasing from caput to cauda. A major fraction their respective fluid. appeared only mature rats, rabbits...
SUMMARY Calreticulin (CalR), a Ca 2+ binding multifunctional protein, is secreted by the parasitic nematode Haemonchus contortus . We have earlier observed of this protein to 24‐kDa polypeptide (p24) present in an enriched preparation prothrombin. In study, identity p24 was established as C‐reactive (CRP) several criteria. CalR CRP elegant strategy devised parasite survive host. The may achieve either limiting free concentration CRP, which has antiparasite activity or inhibit activation...
Treatment with cadmium chloride (CdCl2) and cyproterone acetate (CA) depressed angiotensin converting enzyme (ACE) activity significantly in testes epididymal regions of the adult rats compared to corresponding untreated controls. Exogenous testosterone CA-treated increased both epididymis, effect latter being very significant comparable Testosterone failed induce ACE caput epididymis 30 day-old immature rats, but was detected corpus cauda epididymis. Our findings indicate that testicular...
PCR was used to diagnose toxoplasmosis in two pairs of Barbari goats infected by oral administration doses either 10 4 or 5 oocysts Toxoplasma gondii . Blood and lymph node aspirates were collected from the control goat at intervals, tissues also a fetus that aborted doe died during trial. Both processed unprocessed samples for PCR, using primers directed multicopy B1 gene. None blood positive, but specific signal obtained after partial DNA extraction. Direct lung, muscle mesenteric lung...