A5003201322- Hippo pathway signaling and YAP/TAZ
- Diverse Scientific and Economic Studies
- Cellular Mechanics and Interactions
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Endoplasmic Reticulum Stress and Disease
- Melanoma and MAPK Pathways
- Radiopharmaceutical Chemistry and Applications
- Prostate Cancer Treatment and Research
- Renal and related cancers
- Sirtuins and Resveratrol in Medicine
- Ubiquitin and proteasome pathways
- PARP inhibition in cancer therapy
- Cell Image Analysis Techniques
- Cell Adhesion Molecules Research
- Adenosine and Purinergic Signaling
- Diabetes and associated disorders
- Bioinformatics and Genomic Networks
- Autophagy in Disease and Therapy
- PI3K/AKT/mTOR signaling in cancer
- Colorectal Cancer Treatments and Studies
- Chemical synthesis and pharmacological studies
- Protein Kinase Regulation and GTPase Signaling
Institute of Cancer Research
2013-2024
University College London
2021-2023
Institute of Cancer Research
2021
Growing evidence supports a role for deficient Wnt signalling in Alzheimer's disease (AD). First, the antagonist DKK1 is elevated AD brains and required amyloid-β-induced synapse loss. Second, LRP6 co-receptor integrity three variants of this receptor are linked to late-onset AD. However, expression/role other components remain poorly explored receptors Frizzled1 (Fzd1), Fzd5, Fzd7 Fzd9 interest due their formation/plasticity. Our analyses showed reduced FZD1 FZD7 mRNA levels hippocampus...
Abstract In order to metastasise, triple negative breast cancer (TNBC) must make dynamic changes in cell shape. The shape of all eukaryotic cells is regulated by Rho Guanine Nucleotide Exchange Factors (RhoGEFs), which activate Rho-family GTPases response mechanical and informational cues. contrast, GTPase-activating proteins (RhoGAPs) inhibit GTPases. However, RhoGEFs RhoGAPS couple TNBC their environment very poorly understood. Moreover, whether the activity particular RhoGAPs become...
The function and capacity of the endoplasmic reticulum (ER) is determined by multiple processes ranging from local regulation peptide translation, translocation, folding, to global changes in lipid composition. ER homeostasis thus requires complex interactions amongst numerous cellular components. However, describing networks that maintain during cell behavior environmental fluctuations has, date, proven difficult. Here we perform a systems-level analysis homeostasis, find although signaling...
Proper localization of receptors for synaptic organizing factors is crucial synapse formation. Wnt proteins promote assembly through Frizzled (Fz) receptors. In hippocampal neurons, the surface and Fz5 regulated by neuronal activity, but mechanisms involved remain poorly understood. Here, we report that all Fz can be post-translationally modified S-acylation S-acylated on three C-terminal cysteines zDHHC5. essential to cell surface, axons, presynaptic sites. Notably, S-acylation-deficient...
The concentration of many transcription factors exhibits high cell-to-cell variability due to differences in synthesis, degradation, and cell size. Whether the functions these are robust fluctuations concentration, how this may be achieved, is poorly understood. Across two independent panels breast cancer cells, we show that average whole YAP decreases as a function area. However, nuclear distribution remains constant across cells grouped by size, 4-8 fold size range, implying unperturbed...
The concentration of many transcription factors exhibit high cell-to-cell variability due to differences in synthesis, degradation, and cell size. How these are robust fluctuations is poorly understood. Here we quantified the single levels YAP/TAZ transcriptional co-activators parallel with morphology for over 400,000 cells across 17 lines. We show whole sub-scales respect size as grow during proliferation. However, mean nuclear remains constant cycle. Theoretical modelling demonstrates that...
ABSTRACT Growing evidence supports a role for deficient Wnt signalling in Alzheimer′s disease (AD). First, the antagonist DKK1 is elevated AD brains and required amyloid-β-induced synapse loss. Second, LRP6 co-receptor integrity three variants of this receptor are linked to late-onset AD. However, expression/role other components remain poorly explored receptors Frizzled1 (Fzd1), Fzd5, Fzd7 Fzd9 interest due their formation/plasticity. Our analyses showed reduced FZD1 FZD7 mRNA levels...
Abstract YAP and TAZ are transcriptional co-activators that often constitutively active in triple negative breast cancer (TNBC) cells driving proliferation, invasion, drug resistance. Through multiplexed quantitative genetic screens for YAP/TAZ localisation cell shape, we found the RhoGEF DOCK5 is essential activation metastatic required maintenance of polarity during migration. regulates shape thus through different interactions with CDC42, RAC, RHOA GTPases. focal adhesion (FA)...
YAP and TAZ are transcriptional co-activators that often constitutively active in triple negative breast cancer (TNBC) cells driving proliferation, invasion, drug resistance. Through multiplexed quantitative genetic screens for YAP/TAZ localisation cell shape, we found the RhoGEF DOCK5 is essential activation metastatic required maintenance of polarity during migration. regulates shape thus through different interactions with CDC42, RAC, RHOA GTPases. focal adhesion (FA) morphogenesis...