A5008905531- Enzyme Structure and Function
- Chemical Synthesis and Analysis
- Microbial Natural Products and Biosynthesis
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Synthesis and Characterization of Pyrroles
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Fungal Biology and Applications
- Photosynthetic Processes and Mechanisms
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Algal biology and biofuel production
- Chemical synthesis and alkaloids
- Advanced Synthetic Organic Chemistry
- Plant-Microbe Interactions and Immunity
- Synthesis and Biological Evaluation
- Plant biochemistry and biosynthesis
- Carbohydrate Chemistry and Synthesis
- Gout, Hyperuricemia, Uric Acid
- Synthesis and biological activity
- Synthetic Organic Chemistry Methods
- Biochemical and Molecular Research
- Organic Chemistry Synthesis Methods
- Slime Mold and Myxomycetes Research
University of California, San Diego
2013-2017
University of California System
2016
University of Richmond
2010-2013
University of Montana
2008
Hofstra University
2008
Acyl carrier proteins (ACPs) play a central role in acetate biosynthetic pathways, serving as tethers for substrates and growing intermediates. Activity structural studies have highlighted the complexities of this role, protein-protein interactions ACPs recently come under scrutiny regulator catalysis. As existing methods to interrogate these fallen short, we sought develop new tools aid their study. Here describe design, synthesis, application pantetheinamides that can cross-link with...
In both eukaryotes and prokaryotes, fatty acid synthases are responsible for the biosynthesis of acids in an iterative process, extending by two carbon units every cycle.
An ideal target for metabolic engineering, fatty acid biosynthesis remains poorly understood on a molecular level. These carrier protein-dependent pathways require fundamental protein–protein interactions to guide reactivity and processivity, their control has become one of the major hurdles in successfully adapting these biological machines. Our laboratory developed methods prepare acyl proteins (ACPs) loaded with substrate mimetics cross-linkers visualize trap partner enzymes, we continue...
The synthesis, biological evaluation and molecular modeling of a series pyrrole compounds related to 3,5-dibromo-4-(3,4-dimethoxyphenyl)-1H-pyrrole-2-carboxylic acid that evaluates optimizes C-4 substituents are reported. key factor for microtubule depolymerization activity appears be the presence an appropriately positioned acceptor Cys241β in otherwise hydrophobic subpocket A.
Abstract In fungal non‐reducing polyketide synthases (NR‐PKS) the acyl‐carrier protein (ACP) carries growing intermediate through iterative rounds of elongation, cyclization and product release. This process occurs a controlled, yet enigmatic coordination ACP with its partner enzymes. The transient nature interactions these catalytic domains imposes major obstacle for investigation influence protein–protein on outcome. To further our understanding about how interacts template (PT) domain...
Eukaryotes and prokaryotes possess fatty acid synthase (FAS) biosynthetic pathways that comprise iterative chain elongation, reduction, dehydration reactions. The bacterial FASII pathway differs significantly from human FAS is a long-standing target for antibiotic development against Gram-negative bacteria due to differences the FAS, several existing antibacterial agents are known inhibit enzymes. N-Acetylcysteamine (NAC) thioesters have been used as mimics of natural acyl carrier protein...
Over the past decade, mechanistic crosslinking probes have been used to study protein-protein interactions in natural product biosynthetic pathways. This approach is highly interdisciplinary, combining elements of protein biochemistry, organic chemistry, and computational docking. The development an experiment engage undergraduate students multidisciplinary research described that leverages conformations interactions. provides with a platform learn chemoenzymatic synthesis, polyacrylamide...
Abstract In fungal non‐reducing polyketide synthases (NR‐PKS) the acyl‐carrier protein (ACP) carries growing intermediate through iterative rounds of elongation, cyclization and product release. This process occurs a controlled, yet enigmatic coordination ACP with its partner enzymes. The transient nature interactions these catalytic domains imposes major obstacle for investigation influence protein–protein on outcome. To further our understanding about how interacts template (PT) domain...
ADVERTISEMENT RETURN TO ISSUEPREVFirst ReactionsNEXTTraffic Control in Modular Polyketide SynthasesLowry et al. greatly enhance the mechanistic understanding of vectorial nature PKS machines utilizing ACP cross-linking probes.Kara Finzel and Michael D. BurkartView Author Information Department Chemistry Biochemistry, University California, San Diego, 9500 Gilman Drive, La Jolla, California 92093, United StatesE-mail: [email protected]Cite this: ACS Cent. Sci. 2016, 2, 1, 9–11Publication Date...
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