A5088422944- Glycosylation and Glycoproteins Research
- Glycogen Storage Diseases and Myoclonus
- Lysosomal Storage Disorders Research
- Carbohydrate Chemistry and Synthesis
- Molecular Biology Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Galectins and Cancer Biology
- Biochemical and Molecular Research
- Monoclonal and Polyclonal Antibodies Research
- Trypanosoma species research and implications
- Cancer, Lipids, and Metabolism
- Iron Metabolism and Disorders
- Eicosanoids and Hypertension Pharmacology
- Trace Elements in Health
- Nitric Oxide and Endothelin Effects
- Polyamine Metabolism and Applications
- Mosquito-borne diseases and control
- Digestive system and related health
- Attention Deficit Hyperactivity Disorder
- Ubiquitin and proteasome pathways
- Polysaccharides and Plant Cell Walls
- Advanced biosensing and bioanalysis techniques
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
Institute of Chemistry of the Slovak Academy of Sciences
2015-2025
Slovak Academy of Sciences
2015-2025
Vysoká Škola Zdravotníctva a Sociálnej Práce sv. Alžbety
2020
National Institute of Cardiovascular Diseases
2020
Comenius University Bratislava
2020
The construction of a sensitive electrochemical lectin-based immunosensor for detection prostate specific antigen (PSA) is shown here. Three lectins with different carbohydrate specificities were used in this study to glycoprofile PSA, which the most common biomarker cancer (PCa) diagnosis. biosensor showed presence α-L-fucose and α-(2,6)-linked terminal sialic acid within PSA´s glycan high abundance, while only traces α-(2,3)-linked found. MALDI TOF/TOF mass spectrometry was applied...
Changes in protein glycosylation are associated with most biological processes, and the importance of glycomic analysis research disorders is constantly increasing, including neurodevelopmental field. We glycoprofiled sera 10 children attention-deficit hyperactivity disorder (ADHD) matching healthy controls for 3 types samples: whole serum, after depletion abundant proteins (albumin IgG), isolated IgG. The analytical methods used were a lectin-based glycoprotein microarray enabling...
SUMMARY We report on the homo‐ and hetero‐transglycosylation activities of HvXET3 HvXET4 xyloglucan xyloglucosyl transferases (XET; EC 2.4.1.207) from barley ( Hordeum vulgare L.), visualisation these in young roots using Alexa Fluor 488‐labelled oligosaccharides. discover that isozymes catalyse transglycosylation reactions with chemically defined donor acceptor substrates, specifically penta‐galacturonide [α(1‐4)GalA p ] 5 – homogalacturonan (pectin) fragment. This activity is supported by...
Background: Aberrant glycosylation is a hallmark of cancer and thereby has an excellent potential for the discovery novel biomarkers. Impairments in glycan composition lipoproteins impact their functional properties can be associated with various diseases, including cancer. This research still its infancy; however, it lead to development new diagnostic disease stratification approaches as well therapeutic strategies. Therefore, we aimed evaluate anomalies O-glycosylation apolipoprotein C-III...
Congenital disorders of glycosylation (CDG) are a group rare inherited metabolic caused by defect in the process protein glycosylation. In this work, we present comprehensive glycoprofile analysis male patient with novel missense variant SLC35A2 gene, coding galactose transporter that translocates UDP-galactose from cytosol to lumen endoplasmic reticulum and Golgi apparatus. Isoelectric focusing serum transferrin, which resulted CDG type II pattern, was followed structural transferrin...
The brain stem contains important nuclei that control cardiovascular function via the sympathetic nervous system (SNS), which is strongly influenced by nitric oxide. Its biological activity also largely determined oxygen free radicals. Despite many experimental studies, role of AT1R-NAD(P)H oxidase-superoxide pathway in NO-deficiency not yet sufficiently clarified. We changes radical signaling and antioxidant detoxification response young adult Wistar rats during chronic administration...
Congenital disorder of glycosylation type Ig (ALG12-CDG) is a rare inherited metabolic disease caused by defect in alpha-mannosyltransferase 8, encoded the ALG12 gene (22q13.33). To date, only 15 patients have been diagnosed with ALG12-CDG globally. Due to newborn Slovak patient's clinical and biochemical abnormalities, isoelectric focusing transferrin was performed observed significant hypoglycosylation typical CDG I. Furthermore, analysis neutral serum N-glycans mass spectrometry revealed...
Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by a unique, multilayered cell wall, which offers bacterium high level protection from attacks host immune system. The primary structure wall core, composed covalently linked peptidoglycan, branched heteropolysaccharide arabinogalactan, and mycolic acids, well known, numerous enzymes involved biosynthesis its components are characterized. biogenesis takes place at both cytoplasmic periplasmic faces...
Background: Alpha-mannosidosis is a rare lysosomal storage disorder, caused by decreased activity of α-D-mannosidase. This enzyme involved in the hydrolysis mannosidic linkages N-linked oligosaccharides. Due to mannosidase defect, undigested mannose-rich oligosaccharides (Man2GlcNAc - Man9GlcNAc) accumulating cells are excreted large quantities urine. Methods: In this work, we determined levels urinary patient subjected novel replacement therapy. Urinary were extracted using solid phase...
AIM: Congenital disorders of glycosylation (CDG) belong to an expanding group rare genetic metabolic caused by defects in the complex chemical enzymatic process glycosylation.The study is aimed at presenting a case report premature dysmorphic newborn, clinical presentation condition, way it was diagnosed and treated, as well its comparison with known cases.RESULTS: The result glycan analysis supports assumption supposed disorder also specifi es c subtype: CDG-1, subtype ALG12-CDG...
We investigated the use of boron-doped diamond (BDD) with different surface morphologies for enhanced detection nine peptides by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS). For first time, we compared three nanostructured BDD film (Continuous, Nanograss, and Nanotips) differently terminated surfaces (-H, -O, -F) to commercially available Ground Steel plates. All these were evaluated their effectiveness in detecting MALDI-MS. Our results demonstrated that certain...
Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disorder (LSD) caused by deficiency of N-sulphoglucosamine sulphohydrolase, which one four enzymes involved in heparan sulfate degradation. Traditional methods used for MPS diagnostics usually constitute selective screening, based on the analysis urinary glycosaminoglycans, further enzymatic assays leukocytes, and mutation analysis. Nowadays, some LSDs, including mucopolysaccharidoses, can be precisely diagnosed mass...
The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis vertebrates together with the exporter ferroportin. Mutations CP gene give rise to aceruloplasminemia, rare neurodegenerative disease for which no cure is available. Many aspects of (patho)physiology are still unclear and would benefit from availability recombinant protein structural functional studies. Furthermore, could be evaluated enzyme replacement therapy treatment aceruloplasminemia. We report production...
Cholera is a life-threatening diarrhoeal disease caused by ingestion of Vibrio cholerae. There are at least 200 serogroups V. cholerae but only two them causing epidemics - O1 and O139 serogroups. Fragmentation analysis O-antigen, also known as O-specific polysaccharide (OSP), from lipopolysaccharide (LPS) important to obtain new information about its structure, such fragmentation patterns fragment structures. In the present study, OSP core (OSPc) structure was studied using matrix-assisted...