A5068922225- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Diabetes and associated disorders
- Plant tissue culture and regeneration
- T-cell and B-cell Immunology
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
Lomonosov Moscow State University
2017-2023
Moscow State University
2018
The biopharmaceutical industry is significantly growing worldwide, and the Chinese hamster ovary (CHO) cells are used as a main expression host for production of recombinant monoclonal antibodies. Various metabolic engineering approaches have been investigated to generate cell lines with improved characteristics increasing longevity mAb production. A novel culture method based on 2-stage selection makes it possible develop stable line high-quality production.We constructed several design...
Seasonal and highly infectious strains of the influenza A B viruses cause millions cases severe complications in elderly people, children, patients with immune diseases each year. Immunoglobulin (IgA), which is an active component humoral immunity, can prevent spread virus upper respiratory tract. The preparation study properties recombinant virus-specific IgA could be important approach to finding new means preventing treating influenza. Based on CHO DG44 cells, we developed stable...
Background: Immunoglobulin (Ig) G is the most commonly used therapeutic antibodies. Recently, interest in IgA antibodies to treat respiratory infectious diseases has been increasing. The reason for inefficient use of recombinant antibody aggregation cell culture, affecting longevity and productivity lines. Lactate an important metabolite that affects cultivation stable lines producing monoclonal Methods: In present study, we investigated whether different combinations succinic acid...
We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins the influenza A virus. On basis a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for expression heavy and light chains immunoglobulins IgA1-, IgA2m1-, IgG-isotypes. Following transfection selection, stable Chinese hamster ovary (CHO) cell lines were produced. The antibodies IgG-isotypes purified from culture media. performed an immunochemical characterization studied their...
We obtained recombinant variants of human antibody FI6 broadly specific to hemagglutinins the influenza A virus.On basis a bi-promoter (CMV, hEF1-HTLV) vector, we developed genetic constructs for expression heavy and light chains immunoglobulins IgA1-, IgA2m1-, IgG-isotypes.Following transfection selection, stable Chinese hamster ovary (CHO) cell lines were produced.The antibodies IgG-isotypes purified from culture media.We performed an immunochemical characterization studied their...