Data on integrase inhibitor resistance come primarily from clinical trials and in vitro studies. We examined results of all clinically indicated genotypic tests (GRTs) performed at a US national referral lab 2009 through 2012.Integrase sequences demographic data were compiled with paired protease-reverse transcriptase (PR-RT) GRT results, when available. Analyses utilized the Stanford HIV Drug Resistance Database. "Major" mutations included T66AIK, E92QV, F121Y, Y143CHR, S147G, Q148HKR,...

To study the prevalence and risk factors for cardiac autonomic neuropathy (CAN) utility of prolongation corrected QT interval (QTc) in ECG to diagnose CAN patients with diabetes mellitus.Cross-sectional conducted among attending diabetic clinic a teaching hospital.The 100 type 1 2 mellitus was assessed by five function tests Eving's methodology. The score each patient its relationship QTc were analysed. Possible influences age, duration coexistent peripheral on occurrence also studied.The...

Oral lichen planus (OLP) is an immune-mediated potentially malignant disorder of the oral cavity. Dysplastic OLP has altered cytogenic profile and can progress into squamous cell carcinoma. The epidemiology well-described in several relatively large series from various geographic locations, whereas such southern India rare. aim present study was to determine a cohort South Indian population.All case data records 29,606 patients who visited Mar Baselios Dental College Hospital, Kerala, 2014...

The signaling pathways associated with estrogen-induced proliferation of epithelial cells in the reproductive tract have not been defined. To identify receptor tyrosine kinases that are activated vivo by 17 beta-estradiol (E2), uteri from ovariectomized mice were examined for enhanced phosphorylation various receptors and a substrate following treatment this hormone. Within 4 hr after hormone exposure, extracts showed increased phosphotyrosine (P-Tyr) immunoreactivity at several bands,...

Despite prevention efforts, new HIV diagnoses continue in the southern United States, where epidemic is characterized by significant racial/ethnic disparities. We integrated phylogenetic analyses with clinical data to reveal trends local transmission.Cross-sectional analysis of 1671 HIV-infected individuals each one B-subtype pol sequence obtained during chronic (82%; UNC Center for AIDS Research Clinical Cohort) or acute/recent (18%; Duke/UNC Acute Consortium) infection.Phylogenies were...

The PHR1 gene of Saccharomyces cerevisiae encodes a photolyase which repairs specifically and exclusively pyrimidine dimers, the most frequent lesions induced in DNA by far-UV radiation. We have asked whether expression is modulated response to UV-induced damage DNA-damaging agents that induce structurally dissimilar dimers. Using PHR1-lacZ fusion beta-galactosidase regulated 5' regulatory elements, we found exposure cells 254-nm light, 4-nitroquinoline-N-oxide, methyl methanesulfonate,...

The relationship between hyperglycemia and arterial recanalization following intravenous recombinant tissue-plasminogen activator treatment in acute ischemic stroke is not well understood.We aimed to evaluate the effects of thrombolysed patients on rate clinical outcome.We studied 348 (231 subjects from CLOTBUST databank 117 trial phase II) with documented intracranial artery occlusion treated activator. Serum glucose was determined at baseline before administration. Hyperglycemia defined as...

Previous studies from our laboratory demonstrated that a transgene consisting of the promoter for ovine FSH β-subunit gene and luciferase reporter (wt-oFSHβLuc) was expressed regulated like FSHβ in vivo vitro. In present study pituitary cultures were prepared these transgenic mice as well carrying mutated oFSHβLuc lacking two functional activator protein-1 (AP-1) sites at −120 −83 bp (mut-oFSHβLuc). These AP-1 reported necessary induction by GnRH HeLa cell system. To examine importance...

Abstract FSH is an α/β heterodimeric glycoprotein, the formation of which regulated primarily by expression its β-subunit. Recent studies on transcriptional regulation ovine β-subunit gene (oFSHβ) have defined two functional activating protein-1 (AP-1) enhancers in proximal promoter (located at −120 and −83 bp) that are probably physiologically important for FSHβ expression. As GnRH a major regulator also known to stimulate synthesis Jun Fos family members (AP-1), we investigated possibility...

Objective To estimate the change in intraoral volume following orthodontic extraction correction bidentoalveolar protrusion cases and to establish whether there is a significant associated with above treatment. Materials Methods This study was conducted on 15 patients protrusion. Data were collected using cone beam computed tomography before after correction. Pretreatment post-treatment determined for each patient from multiplanar reconstructions Planmeca Romexis viewer software. Results The...

Background Transmitted drug resistance (TDR) limits antiretroviral options and thus complicates the management of HIV-positive patients. HIV disproportionately affects southern US, but available national estimates TDR prevalence principally reflect large metropolitan centres outside this region. Methods The Duke/UNC Acute Program has collected data on acute or recent infections (ARHI) in North Carolina since 1998. represent antibody-negative, RNA-positive Recent infection was determined by...

Few studies have investigated the risk factors associated with developing intestinal stoma complications using appropriate multivariable methods. We aimed to determine prevalence of, and for, stomal complications.A retrospective, case-control methodology was used investigate 12 explanatory variables four outcome in 202 consecutive patients receiving stomas a district general hospital United Kingdom between January 2013 December 2014. Univariable logistic regression were calculate odds ratios...

Managing mild to moderate Class II malocclusion is a one of the common and major challenges orthodontists. discrepancies with mandibular deficiency during active growth are usually treated by myofunctional appliances. Fixed functional appliances evolved due noncompliance conventional This case report illustrates efficiency PowerScope in correction skeletal patient aged 13 years who has reported department chief complaint forwardly placed upper front teeth. jaw retrusion was initially MBT...

Some of the actions estradiol occur through stimulation growth factor pathways in target organs. Tyrosine-phosphorylated (Tyr(P)) insulin-like factor-1 receptor (IGF-1R) and insulin substrate (IRS)-1 are found uterus mice treated with estradiol. Immunoprecipitates uterine Tyr(P) IRS-1 contained both p85, regulatory subunit phosphatidylinositol (PI) 3-kinase, PI 3-kinase catalytic activity. Estradiol also stimulated binding to IGF-1R. Depletion from extracts reduced associated receptor, which...

Transgenic mice harboring the ovine FSHβ (oFSHβ) promoter plus first intron (from −4741 to +759 bp) linked a luciferase reporter gene (oFSHβLuc) were generated determine whether this can direct tissue-specific expression in vivo and serve as model for studying hormonal regulation of gene. Of six lines transgenic analyzed, was detected uniquely pituitaries five them. Pituitary activity decreased 51–99% by chronic GnRH treatment (Lupron depot). Orchidectomy caused 2- 8-fold increase,...

Background: Partner notification is an important component of public health test and treat interventions. To enhance this essential function, we assessed the potential for molecular methods to supplement routine partner corroborate HIV networks. Methods: All persons diagnosed with infection in Wake County, NC, during 2012–2013 their disclosed sexual partners were included a network. A data set containing HIV-1 pol sequences collected NC 1997–2014 from 15,246 was matched HIV-positive network...

The PHR1 gene of Saccharomyces cerevisiae encodes the DNA repair enzyme photolyase. Transcription increases in response to treatment cells with 254-nm radiation and chemical agents that damage DNA. We report here identification a damage-responsive binding protein, termed photolyase regulatory protein (PRP), its cognate site, upstream repression sequence, together regulate induction transcription after damage. PRP activity, monitored by electrophoretic-mobility-shift assay, was detected...

Our goal is to assess if current antiplatelet (AP) use has an effect on recanalization rate and outcome in acute stroke patients.We conducted a retrospective analysis of patients who received intravenous (IV) recombinant tissue plasminogen activator (rt-PA) had transcranial Doppler examination within 3 h symptom onset. The TCD findings were interpreted using the Thrombolysis Brain Ischemia flow grading system as persistent arterial occlusion, reocclusion or complete recanalization. Complete...

Background The integration of traditional contact tracing with HIV sequence analyses offers opportunities to mitigate some the barriers effective network construction. We used combined during an outbreak investigation spatiotemporally clustered acute infections evaluate if observed clustering was product a single outbreak. Methods investigated and recent index cases reported in North Carolina from 2013 2014 their contacts. Contact networks were constructed surveillance data compared...