A5048612387- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Lipid metabolism and biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Plant Genetic and Mutation Studies
- Biocrusts and Microbial Ecology
- Microbial Metabolic Engineering and Bioproduction
- Plant responses to water stress
- Plant biochemistry and biosynthesis
- Mitochondrial Function and Pathology
- Advanced Proteomics Techniques and Applications
- Plant Gene Expression Analysis
- Plant Reproductive Biology
- Catalysis for Biomass Conversion
- Plant Parasitism and Resistance
- Bioactive natural compounds
- Fungal and yeast genetics research
- Cocoa and Sweet Potato Agronomy
- Wheat and Barley Genetics and Pathology
Institute of Botany
2008-2022
Chinese Academy of Sciences
2008-2022
Beijing Botanical Garden
2013-2014
University of Tsukuba
1999-2004
Institute of Agrobiological Sciences
2003
Construction of metabolic and regulatory pathways from proteomic data can contextualize the large-scale within overall physiological scheme an organism. It is efficient way to predict phenotype or style. We did protein profiling in germinating rice seeds through 1-DE via LC MS/MS shotgun strategy. In total, 673 proteins were identified, could be sorted into 14 functional groups. The largest group was metabolism related. integrated different show style reserves mobilization precursor...
The technique of proteome analysis with two-dimensional PAGE has the power to monitor global changes that occur in protein expression tissues and organisms and/or occurs under stresses. In this study, catalogues rice were constructed, a functional characterization some these proteins was examined. Proteins extracted from various kinds stress separated by PAGE. An image analyzer used reveal total 10,589 spots on 10 gels stained Coomassie Brilliant Blue. electroblotted onto polyvinylidene...
Jatropha curcas is an important economic plant for biodiesel, which extracted mainly from the endosperm of its mature seeds. Despite morphological and functional differences between embryo endosperm, proteomic characteristics two tissues are not yet known. Similar profiles were observed in two-dimensional gel electrophoresis maps tissues. There 380 533 major protein spots respectively. Fourteen identical spots, showing a notable change, selected identified by tandem mass spectrometry. Among...
Phytosulfokine-α (PSK-α) is a novel plant peptidyl growth factor that was originally isolated from the conditioned medium of cultures asparagus mesophyll cells and shown to stimulate cell division. To date, however, there have been no reports on effects PSK-α morphogenesis in higher plants. Therefore, this study, we examined formation adventitious buds calli Antirrhinum majus. The induced hypocotyl-derived callus had formed solid Murashige Skoog's (MS) containing 1.2μM naphthoxyacetic acid....
A new full‐length cDNA encoding an AP2/EREBP domain‐containing transcription factor named AoDREB was isolated from Asparagus officinalis L. using the RACE‐PCR method. It is a homolog to dehydration‐responsive element binding protein (DREB) and classified A6 subgroup of DREB subfamily. Using yeast one‐hybrid system, we conducted DRE assay demonstrated that can bind specifically. transcriptional activity showed capable activating expression reporter gene in yeast. RT‐PCR analysis revealed...
The genetic basis of plant local adaptation has been extensively studied, yet the interplay between adaptation, divergence, and microbial community remains unclear. Our study used restriction-site associated DNA sequencing (RAD-seq) approach to explore divergence in Broussonetia papyrifera internal transcribed spacers (ITS) characterize fungal community. RAD-seq results show that B. individuals could be divided into three genotypes; this genotyping result was consistent with classification...
Protein extraction is a crucial step for proteomics studies. To establish an effective protein protocol suitable two‐dimensional electrophoresis (2 DE ) analysis in J erusalem artichoke ( H elianthus tuberosus L .), three different methods—trichloroacetic acid/acetone, M g/ NP ‐40, and phenol/ammonium acetate—were evaluated using leaves as source materials. Of the methods, trichloroacetic acid/acetone yielded best separation pattern highest number of spots 2 analysis. Proteins highly...
In carrot (Daucus carota L.) somatic embryos, desiccation tolerance is induced by treatment with abscisic acid (ABA). Six cDNA clones that showed ABA-enhanced expression were isolated from differential screening ABA-treated and ABA-untreated they named the CAISE (carrot ABA-induced in embryos) genes. Five of encode late embryogenesis abundant (LEA) proteins, other clone encodes a glucose ribitol dehydrogenase. The genes was detected maturing seeds, embryogenic cells, embryos which exhibit...