A5031196636- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- S100 Proteins and Annexins
- Bone Tissue Engineering Materials
- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Neurotoxicity Research
- Neurological Disease Mechanisms and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Neurological Disorders and Treatments
- Ubiquitin and proteasome pathways
- Medical and Biological Ozone Research
- Biochemical effects in animals
- Tissue Engineering and Regenerative Medicine
- Biotin and Related Studies
- Hemoglobin structure and function
- Cell Image Analysis Techniques
- Traumatic Brain Injury and Neurovascular Disturbances
- Curcumin's Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Alzheimer's disease research and treatments
- Erythrocyte Function and Pathophysiology
- Intensive Care Unit Cognitive Disorders
- Genetic Neurodegenerative Diseases
- Thallium and Germanium Studies
First Pavlov State Medical University of St. Petersburg
2019-2025
Peter the Great St. Petersburg Polytechnic University
2020
Institute of Experimental Medicine
2015-2019
Research Institute of Human Morphology
2015-2018
Czech Academy of Sciences, Institute of Experimental Medicine
2013-2017
Morpho (United States)
2015
Russian Academy of Sciences
2011-2014
Academy of Medical Sciences
2011-2014
Tissue fixation is critical for immunohistochemistry. Recently, we developed a zinc-ethanol-formalin fixative (ZEF), and the present study was aimed to assess applicability of ZEF human brain histology immunohistochemistry evaluate detectability different antigens in fixed with ZEF. In total, 11 were tested, including NeuN, neuron-specific enolase, GFAP, Iba-1, calbindin, calretinin, choline acetyltransferase, glutamic acid decarboxylase (GAD65), tyrosine hydroxylase, synaptophysin,...
The aim was to conduct a comparative pathomorphological analysis of the toxic effects new spirofused heterocyclic compounds on rat hippocampus. Material and methods . Male Wistar rats were divided into 3 groups. Animals control group injected with 1 ml saline solution, 1st experimental (EG1) given single intraperitoneal injection barbiturate, animals 2nd (EG2) administered oxindole at dose 12 mg/kg animal’s body weight, also in volume ml. 2 8 weeks after administration drugs, taken out...
Cerium oxide nanoparticles (CeONPs), as part of tissue regeneration matrices, can protect cells from reactive oxygen species and oxidative stress. In addition, they influence the properties scaffold, including its electrospinnability mechanical strength. this work, we prepared electrospun fiber mats a chitosan polyethylene blend (CS-PEO) with addition ceria (CS-PEO-CeONP). The CeONPs resulted in smaller diameter higher swelling compared to CS-PEO mats. CeONP-modified also had Young’s modulus...
New tubular conduits have been developed for the regeneration of peripheral nerves and repair defects that are larger than 3 cm. The consist a combination poly(L-lactide) nanofibers chitosan composite fibers with chitin nanofibrils. In vitro studies were conducted to assess biocompatibility using human embryonic bone marrow stromal cells (FetMSCs). revealed good adhesion differentiation on just one day after cultivation. Furthermore, an in vivo study was carried out evaluate...
BACKGROUND: Severe progressive forms of infectious keratitis are often associated with the risk violation structural integrity eyeball and development endophthalmitis. In presence negative dynamics against background conservative etiotropic therapy for severe keratitis, it is advisable to use surgical methods treatment. The combination two influencing a pharmacoresistant process in cornea — cross-linking fulguration promising requires further investigation.
 AIM: experiment, evaluate...
Introduction . Ischemic postconditioning (IPostC) is a new concept in the brain protection strategy. Almost all researches this area focus on functioning and survival of neurons, while non-neuronal cells affected by IPostC remain unexplored. The aim to study effect changes microglia neocortex Wistar rats after global ischemia during various periods reperfusion. Materials methods Male were used as model 10-minute with subsequent IPostC; reperfusion-ischemia cycle was 15 s/15 s. In early (2...
We studied the intranuclear localization of protein nucleophosmin (B23) and ubiquitin in dopaminergic neurons human substantia nigra (n = 6, age 25-87 years) using immunohistochemistry confocal laser microscopy. Intranuclear ubiquitin-immunopositive bodies that morphologically correspond to Marinesco were found be present (tyrosine hydroxylase-immunopositive) but absent non-dopaminergic neurons. The number varied from 0 6 per cell nucleus. Nucleophosmin was neuronal nucleolus, with nucleolus...
High performance liquid chromatography (HPLC) was used to determine doxorubicin (DOX) contents in rat blood after subcutaneous or intraperitoneal administration of the drug loaded into delivery systems (DS) based on nanoparticles poly(amino acids) various compositions.One variant system prepared self-assembled NPs random copolymer L-glutamic acid and D-phenylalanine (P[EF]).The second DS poly-Lserine-b-poly(L-glutamic acid) block (P[SE]); this introduce DOX for first time.Encapsulation...
The present work is focused on the study of target drug delivery systems comprised porous spherical calcium carbonate vaterites (СаСО 3 ) covered with dextran sulfate protective shell.Behavior objects was investigated in vivo.The samples were implanted into rat muscular tissue and removed after different periods exposure (3 days, 1, 2, 4, 12 weeks operation).It shown that certain transformations structure carriers occurred over time, which they underwent bioresorption.In days implantation,...
To determine the cytochemical characteristics of unchanged neurons human substantia nigra using a wide range immunocytochemical markers some which (glutamate decarboxylase-65, PGP 9.5, non-phosphorylated neurofilament proteins, alpa-tubulin) have never been used for study dopaminergic neurons.Fragments midbrain (17 men and women, aged from 28 to 78 years) archives Department General Specific Morphology Institute Experimental Medicine were used. The was performed classical neurohistological...