A5020249008- Nitric Oxide and Endothelin Effects
- Metabolism and Genetic Disorders
- Viral Infections and Vectors
- interferon and immune responses
- Inflammasome and immune disorders
- Ion Transport and Channel Regulation
- Renal Transplantation Outcomes and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Redox biology and oxidative stress
- Air Quality Monitoring and Forecasting
- Air Quality and Health Impacts
- Heart Rate Variability and Autonomic Control
- Genomics, phytochemicals, and oxidative stress
- Sodium Intake and Health
- Blood Pressure and Hypertension Studies
- Long-Term Effects of COVID-19
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Analytical Chemistry and Sensors
- Phagocytosis and Immune Regulation
- Inflammation biomarkers and pathways
- Alzheimer's disease research and treatments
- Endoplasmic Reticulum Stress and Disease
- Renal function and acid-base balance
- Organ Transplantation Techniques and Outcomes
- Viral Infectious Diseases and Gene Expression in Insects
University of Exeter
2014-2025
NIHR Exeter Clinical Research Facility
2017-2020
Royal Devon and Exeter Hospital
2020
Peninsula College of Medicine and Dentistry
2012
Some neurodegenerative diseases have an element of neuroinflammation that is triggered by viral nucleic acids, resulting in the generation type I interferons. In cGAS-STING pathway, microbial and host-derived DNA bind activate sensor cGAS, cyclic dinucleotide, 2'3-cGAMP, binds to a critical adaptor protein, stimulator interferon genes (STING), which leads activation downstream pathway components. However, there limited work demonstrating human diseases.Post-mortem CNS tissue from donors with...
Ingested inorganic nitrate (NO3⁻) has multiple effects in the human body including vasodilation, inhibition of platelet aggregation, and improved skeletal muscle function. The functional oral NO3⁻ involve vivo reduction to nitrite (NO2⁻) thence nitric oxide (NO). However, potential involvement S-nitrosothiol (RSNO) formation is unclear. We hypothesised that RSNO concentration ([RSNO]) red blood cells (RBCs) plasma increased by NO3⁻-rich beetroot juice ingestion. In healthy volunteers, we...
Dietary nitrate (NO3-) supplementation can enhance nitric oxide (NO) bioavailability and lower blood pressure (BP) in humans. The nitrite concentration ([NO2-]) the plasma is most commonly used biomarker of increased NO availability. However, it unknown to what extent changes other congeners, such as S-nitrosothiols (RSNOs), components, red cells (RBC), also contribute BP lowering effects dietary NO3-. We investigated correlations between biomarkers different compartments variables following...
The addition of hydrogen peroxide (H2O2) to cultured cells is widely used as a method modulate redox-regulated cellular pathways, including the induction programmed cell death in culture experiments and testing pro- antioxidant compounds. Here, we assessed effect on response H2O2 pre-adapting squamous carcinoma (A431) standard oxygenation 18.6% O2, compared pre-adapted physiological skin O2 concentration (3.0% O2). We showed that resisted H2O2-induced 3.0% for 96 h prior treatment with H2O2....
Background and Purpose Exposure to nanoparticulate pollution has been implicated in platelet‐driven thrombotic events such as myocardial infarction. Inflammation impairment of NO bioavailability have proposed potential causative mechanisms. It is unclear, however, whether airways exposure combustion‐derived nanoparticles diesel exhaust particles (DEP) or carbon black (CB) can augment platelet aggregation vivo the underlying mechanisms remain undefined. We aimed investigate effects acute lung...
Abstract BACKGROUND Inorganic nitrate from the oxidation of endogenously synthesized nitric oxide (NO) or consumed in diet can be reduced to NO via a complex enterosalivary circulation pathway. The relationship between total exposure by measured urinary excretion and blood pressure large population sample has not been assessed previously. METHODS For this cross-sectional study, 24-hour was spectrophotometry 919 participants InChianti cohort at baseline with mercury sphygmomanometer. RESULTS...
The infiltration of immune cells into the central nervous system mediates development autoimmune neuroinflammatory diseases. We previously showed that loss either Fabp5 or calnexin causes resistance to induction experimental encephalomyelitis (EAE) in mice, an animal model multiple sclerosis (MS). Here we show brain endothelial lacking have increased abundance cell surface CD200 and soluble (sCD200) as well decreased T-cell adhesion. In a tissue culture blood-brain barrier, antagonizing...
Blood pressure (BP) control is a key target for interventions to reduce cognitive decline. This cross-sectional study explored associations between objective (24-hour urine excretion) and subjective (food frequency questionnaire [FFQ]) measures of dietary sodium nitrate intakes with function resting BP in the InCHIANTI cohort. Baseline data from 989 participants aged >50 years were included. In fully adjusted models, concurrent high low (Odds Ratio (OR)=0.49, 95%CI 0.32-0.76, p = 0.001) (OR...
Abstract Background: Some neurodegenerative diseases have an element of neuroinflammation that is triggered by viral nucleic acids, resulting in the generation type I interferons. In cGAS-STING pathway, microbial and host-derived DNA bind activate sensor cGAS, cyclic dinucleotide, 2’3-cGAMP binds to a critical adaptor protein, stimulator interferon genes (STING), which leads activation downstream pathway components. However, there limited work demonstrating cGAS- STING human diseases....
We previously showed that calnexin (Canx)-deficient mice are desensitized to experimental autoimmune encephalomyelitis (EAE) induction, a model is frequently used study inflammatory demyelinating diseases, due increased resistance of the blood-brain barrier immune cell transmigration. also discovered Fabp5, an abundant cytoplasmic lipid-binding protein found in brain endothelial cells, makes protein-protein contact with C-tail domain Canx. Remarkably, both Canx-deficient and Fabp5-deficient...
Abstract Background Early identification and treatment of kidney transplant rejection episodes is vital to limit loss function prolong the life transplanted recipient. Current practice depends on detecting a creatinine rise. A biomarker diagnose at an earlier time point than current practice, or inform decision making biopsy, could be transformative. It has previously been shown that urinary nitrate concentration elevated in renal rejection. Nitrate nitric oxide (NO) oxidation product....
Abstract Background and Aims Early identification treatment of kidney transplant rejection episodes is vital to limit loss function prolong the life transplanted recipient. Current practice depends on detecting a creatinine rise. A biomarker diagnose rejection, either at an earlier time point, or inform decision making biopsy, could be transformative. Urinary nitrate concentration elevated in renal rejection. Nitrate nitric oxide (NO) oxidation product. Transplant upregulates NO synthesis...
Abstract Background Some neurodegenerative diseases have an element of neuroinflammation that is triggered by viral nucleic acids, resulting in the generation type I interferons. In cGAS-STING pathway, microbial and host-derived DNA bind activate sensor cGAS, cyclic dinucleotide, 2’3-cGAMP binds to a critical adaptor protein, stimulator interferon genes (STING), which leads activation downstream pathway components. However, there limited work demonstrating cGAS- STING human diseases. Methods...
The physiological oxygen environment in human tissues vivo (physioxia) typically ranges from 1.0-12.5% O2. However, vitro mammalian cell culture is largely performed under an concentration of 18.6% effect pre-adapting squamous carcinoma cells (A431 cells) to growth O2 on the subsequent cellular response hydrogen peroxide (H2O2), compared A431 pre-adapted physioxia, not well understood. This study showed that resisted (H2O2)-induced death skin physioxia (3.0% O2) for 96 h prior treatment with...