A5046504637- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Neurogenetic and Muscular Disorders Research
- 3D Printing in Biomedical Research
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Cytomegalovirus and herpesvirus research
- Advanced Chemical Sensor Technologies
- Infectious Encephalopathies and Encephalitis
- Listeria monocytogenes in Food Safety
- Biomedical Ethics and Regulation
- Genomics and Chromatin Dynamics
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Mycotoxins in Agriculture and Food
- Muscle Physiology and Disorders
- Competitive and Knowledge Intelligence
- Advanced Proteomics Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Anesthesia and Neurotoxicity Research
- Mass Spectrometry Techniques and Applications
- Physiological and biochemical adaptations
- Cardiac Ischemia and Reperfusion
Medical College of Wisconsin
2012-2023
Lakeland College
2023
University of Wisconsin–Madison
2008-2009
Spinal muscular atrophy (SMA) is a genetic disorder caused by the deletion of survival motor neuron 1 ( SMN1 ) gene that leads to loss neurons in spinal cord. Although are selectively lost during SMA pathology, selective replacement SMN does not lead full rescue mouse models. Due ubiquitous expression SMN, it likely other cell types besides affected its disruption and therefore may contribute disease pathology. Here we show astrocytes SMAΔ7 cord from SMA‐induced pluripotent stem cells...
Spinal muscular atrophy (SMA) is a genetic disorder caused by deletion of the survival motor neuron 1 gene leading to loss, muscle atrophy, paralysis, and death. We show here that induced pluripotent stem cell (iPSC) lines generated from two Type I SMA subjects–one produced with lentiviral constructs second using virus-free plasmid–based approach–recapitulate disease phenotype generate significantly fewer neurons at later developmental time periods in culture compared separate control...
Abstract Using stem cells to replace degenerating muscle and restore lost skeletal function is an attractive therapeutic strategy for treating neuromuscular diseases. Myogenic progenitors are a valuable cell type cell-based therapy also provide platform studying normal development disease mechanisms in vitro. Human pluripotent represent source of tissue generating myogenic progenitors. Here, we present novel protocol deriving from human embryonic (hES) induced (iPS) using free-floating...
Dystrophin-deficient cardiomyopathy is a growing clinical problem without targeted treatments. We investigated whether nicorandil promotes cardioprotection in human dystrophin-deficient induced pluripotent stem cell (iPSC)-derived cardiomyocytes and the muscular dystrophy mdx mouse heart.Dystrophin-deficient iPSC-derived had decreased levels of endothelial nitric oxide synthase neuronal synthase. The increased injury death after 2 hours stress recovery. This was associated with reactive...
Abstract Phosphorylation is universally used for controlling protein function, but knowledge of the phosphoproteome in vertebrate embryos has been limited. However, recent technical advances make it possible to define an organism's at a more comprehensive level. Xenopus laevis offers established advantages analyzing regulation function by phosphorylation. Functionally unbiased, information about would provide powerful guide future studies phosphorylation developmental context. To this end,...
We identified a functional domain (XlePABP2-TRP) of Xenopus laevis embryonic type II poly(A)-binding protein (XlePABP2). The NMR structure XlePABP2-TRP revealed that the is homodimer formed by antiparallel association beta-strands from single RNA recognition motif (RRM) each subunit. In subunit homodimer, canonical site occluded polyproline motif. Upon poly(A) binding, undergoes dimer-monomer transition removes and allows it to be replaced adenosine nucleotides poly(A). Our results provide...
The xCR1 protein is a maternal determinant and cofactor for nodal signaling in vertebrate embryos. accumulates specifically the animal cells of Xenopus embryos, but mRNA distributed equally throughout all embryonic cells. Here, we show that vegetal cell-specific translational repression contributes to this spatially restricted accumulation was associated with polyribosomes not A 351-nucleotide region mRNA's 3′ untranslated sufficient confer pattern translation luciferase reporter by...
Spinal muscular atrophy (SMA), the top genetic cause of infant mortality, is characterized by motor neuron degeneration. Mechanisms underlying SMA pathogenesis remain largely unknown. Here, we report that activity cyclin-dependent kinase 5 (Cdk5) and conversion its activating subunit p35 to more potent activator p25 are significantly up-regulated in mouse models human induced pluripotent stem cell (iPSC) SMA. The increase Cdk5 occurs before onset phenotypes, suggesting it may be an initiator...
University based technology transfer offices function to identify, protect, and market new technologies all of which require strategic decisions about developed at their institutions. Often, limited time resources are available for pursuing patent protection identifying commercial partners licensing. Therefore, determining have high probabilities success is necessary. The collection data regarding the market, referred as competitive intelligence, helps make these decisions. In this article...