A5039270445- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neural Engineering
- 2D Materials and Applications
- Neuroscience and Neuropharmacology Research
- Topological Materials and Phenomena
- Electrochemical Analysis and Applications
- Nicotinic Acetylcholine Receptors Study
- Graphene research and applications
- Iron-based superconductors research
- Inorganic Chemistry and Materials
- Organic and Molecular Conductors Research
- Chalcogenide Semiconductor Thin Films
- Fuel Cells and Related Materials
- Hearing, Cochlea, Tinnitus, Genetics
- Advanced biosensing and bioanalysis techniques
- Receptor Mechanisms and Signaling
- Molecular Junctions and Nanostructures
- Solid-state spectroscopy and crystallography
- Sperm and Testicular Function
- Quantum Dots Synthesis And Properties
- MXene and MAX Phase Materials
- Electronic and Structural Properties of Oxides
- Insect and Pesticide Research
- stochastic dynamics and bifurcation
Renmin University of China
2021-2025
University of Miami
2014-2023
The delicate interplay among the complex intra/inter-layer electron–electron and electron–lattice interactions is fundamental prerequisite of these exotic quantum states, such as superconductivity, nematic order, checkerboard charge order. Here, we explore filling-dependent multiple stable intertwined electronic atomic orders flat-band state 1T-TaS2 encompassing hole phase orders, coexisting left- right-chiral mixed phase/chiral via scanning tunneling microscopy (STM). Combining...
Significance High-conductance Slo1 (BK) K + channels are synergistically activated by Ca 2+ , voltage, Mg and additional factors to modulate membrane excitability in many key physiological processes. of modular design with allosteric interactions between the core transmembrane modules a large cytoplasmic gating ring module, providing model system study principles channel protein function. To examine interactions, we developed constructs that replace module short peptides then characterized...
Large conductance Ca2+-activated K+ channels (BK channels) gate open in response to both membrane voltage and intracellular Ca2+. The channel is formed by a central pore-gate domain (PGD), which spans the membrane, plus transmembrane sensors cytoplasmic gating ring that acts as Ca2+ sensor. How these influence common activation gate, interact with each other, unclear. A previous study showed BK core lacking entire (Core-MT) was devoid of but retained sensitivity (Budelli et al. 2013. Proc....
Large-conductance Ca2+- and voltage-activated K+ (BK) channels have the largest conductance (250–300 pS) of all K+-selective channels. Yet, contributions various parts ion conduction pathway to are not known. Here, we examine contribution entrance inner cavity large conductance. Residues at E321/E324 on each four α subunits encircle cavity. To determine if 321/324 is accessible from pathway, measured single-channel current amplitudes before after exposure wash thiol reagents intracellular...
Large-conductance Ca2+ and voltage-activated K+ (BK) channels control membrane excitability in many cell types. BK are tetrameric. Each subunit is composed of a voltage sensor domain (VSD), central pore-gate domain, large cytoplasmic (CTD) that contains the sensors. While it known activated by Ca2+, activations interact, less about mechanisms involved. We explore here these examining gating contribution an interface formed between VSDs αB helices located at top CTDs. Proline mutations helix...
The molecular basis of a severe developmental and neurological disorder associated with de novo G375R variant the tetrameric BK channel is unknown. Here, we address this question by recording from single channels expressed to mimic mutation heterozygous WT allele. Five different types functional were expressed: 3% consistent WT, 12% homotetrameric mutant, 85% three hybrid (heterotetrameric) assembled both mutant subunits. All except showed marked gain-of-function in voltage activation...
Voltage-dependent gating mechanisms of large conductance Ca2+ and voltage-activated (BK) channels were investigated using two-dimensional maximum likelihood analysis single-channel open closed intervals. To obtain sufficient data at negative as well positive voltages, currents recorded saturating from BK mutated to remove the RCK1 Mg2+ sensors. The acting on bowl sensors resulting BKB increased channel activity while driving into a reduced number states, simplifying model. Five highly...
Abstract BK type Ca 2+ -activated K + channels activate in response to both voltage and . The membrane-spanning sensor domain (VSD) activation binding the cytosolic tail (CTD) open pore across membrane, but mechanisms that couple VSD opening are not clear. Here we show a compound, BC5, identified from silico screening, interacts with CTD-VSD interface specifically modulates dependent mechanism. BC5 activates channel absence of inhibits effects. Thus, perturbs pathway couples allosterically...
BK channels are activated by intracellular Ca2+ and Mg2+ as well depolarization. Such activation is possible because each of the four subunits has two high-affinity sites, one low-affinity site, a voltage sensor. This study further investigates mechanism using single-channel recording to determine separately action on open closed states channel. To limit sites disabled mutation. When stepped from negative holding potentials +100 mV, we find that 10 mM decreases mean latency first channel...
The layered transition metal dichalcogenide $1T\text{\ensuremath{-}}{\mathrm{TaS}}_{2}$ has evoked great interest owing to its particularly rich electronic phase diagram including different charge density wave (CDW) phases. However, few studies have focused on hysteretic transitions based the in-depth discussion of delicate interplay among temperature-dependent interactions. Here, we report a sequence spatial in hysteresis temperature range (160--230 K) via variable-temperature scanning...
Two-dimensional (2D) magnets have attracted significant attentions in recent years due to their importance the research on both fundamental physics and spintronic applications. Here, we report discovery of a new ternary compound FePd2Te2. It features layered quasi-2D crystal structure with one-dimensional Fe zigzag chains extending along b-axis cleavage plane. Single crystals FePd2Te2 centimeter-size could be grown. Density functional theory calculations, mechanical exfoliation atomic force...
Large-conductance, voltage- and Ca2+-activated K+ (BK) channels display near linear current–voltage (I-V) plots for voltages between −100 +100 mV, with an increasing sublinearity more positive potentials. As is the case many types of channels, BK are blocked at potentials by intracellular Ca2+ Mg2+. This fast block progressively reduces single-channel conductance voltage, giving rise to a negative slope in I-V beyond about +120 depending on concentration blockers. In contrast these...
A flat band is an electronic state with quenched kinetic energy and extremely high density of states. In such a state, small electron-electron correlation can drive multiple intriguing quantum phenomena. By using angle-resolved photoemission spectroscopy, we report the existence dispersionless sharp quasiparticle peak below ${E}_{\mathrm{F}}$ in charge wave $4{H}_{b}\text{\ensuremath{-}}{\mathrm{TaS}}_{1.3}{\mathrm{Se}}_{0.7}$. This extends over...
Many intriguing quantum states of matter, such as unconventional superconductivity, magnetic phases and fractional Hall physics, emergent from the spatially-correlated localized electrons in flat band solid materials. By using scanning tunneling microscopy spectroscopy (STM/STS), we report real-space investigation correlated superlattice 4Hb-TaSexS2-x. In contrast with pristine 4Hb-TaS2, selenium (Se) substitutions significantly affect interfacial transfer between CDW 1T- 1H-TaS2 layers,...
Abstract The molecular basis of a severe developmental and neurological disorder associated with de novo G375R variant the tetrameric BK channel is unknown. Here we address this question by recording from single channels expressed for heterozygous mutation. Five different types functional were observed: 3% WT, 12% homomeric mutant, 85% three hybrid channels. All except WT showed marked gain-of-function in voltage activation smaller loss-of-function conductance, both becoming more pronounced...