A5061396220- Photosynthetic Processes and Mechanisms
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
- Escherichia coli research studies
- Electron and X-Ray Spectroscopy Techniques
- Microtubule and mitosis dynamics
- 14-3-3 protein interactions
- Genomics and Chromatin Dynamics
- GaN-based semiconductor devices and materials
- Advanced Electron Microscopy Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Hedgehog Signaling Pathway Studies
- Advancements in Photolithography Techniques
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Cutaneous Melanoma Detection and Management
- Cell death mechanisms and regulation
- Microbial Natural Products and Biosynthesis
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Biomedical and Engineering Education
Institute of Science and Technology Austria
2021-2024
Forschungszentrum Jülich
2024
Paul Scherrer Institute
2017-2022
Novartis (Italy)
2014
University of Turin
2013
Malignant melanoma is one of the most aggressive human cancers, but mechanisms governing its metastatic dissemination are not fully understood. Upregulation miR-214 and ALCAM loss TFAP2 expression have been implicated in this process, with a direct target miR-214. Here, we link as well identify core role for organizing metastasis. upregulated ALCAM, acting transcriptionally through also posttranscriptionally miR-148b (itself controlled by TFAP2), both negative regulators ALCAM. We identified...
Cryo-EM structure of PTCH1-ShhN C24II complex reveals multiple sterol binding sites and a possible translocation pathway.
Significance Serogroup B meningococcus (MenB) causes severe sepsis and invasive meningococcal disease, particularly affecting young children adolescents. The genome-derived vaccine 4CMenB that targets MenB, has now been approved in over 30 countries worldwide. Here we report the crystal structure of trimeric autotransporter Neisserial adhesin A (NadA), one three protein antigens included 4CMenB, epitope mapping a bactericidal mAb monoclonal antibody functional head domain NadA. These results...
Significance Adenylyl and guanylyl cyclases are at the core of cellular signaling. Although molecular mechanisms reactions catalyzed by these enzymes well established, their structures biophysical properties remain only partially characterized. Here, we report structure cytosolic domain a mycobacterial adenylyl cyclase Cya, an evolutionary ancestor mammalian membrane cyclases. The reveals helical domain, highly conserved structural element that links catalytic transmembrane portions Cya. We...
Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 factor binds newly synthesized D1 reaction center polypeptide promotes initial steps PSII assembly, but its binding site is unclear. Here we use cryo-electron microscopy determine structure cyanobacterial D1/D2 complex with attached. Ycf48, 7-bladed beta propeller, amino-acid residues that ultimately ligate...
Mycobacterium tuberculosis adenylyl cyclase (AC) Rv1625c/Cya is an evolutionary ancestor of the mammalian membrane ACs and a model system for studies their structure function. Although vital role in cellular signalling well established, function transmembrane (TM) regions remains unknown. Here, we describe cryo-EM Cya bound to stabilizing nanobody at 3.6 Å resolution. The TM helices 1-5 form structurally conserved domain that facilitates assembly helical catalytic domains. region contains...
As a cell biologist, I, Nicole Amberg, have always been fascinated by type diversity in nature and how ‘form meets function’ for distinct populations. However, when looking at academia, I was discouraged to see that the system does not reflect nature's diverse variable shapes. Instead, academic institutions (and society as whole) still lack homogenous access education, opportunities chances, resulting strong imbalances representation of gender, ethnicity ultimately most importantly,...
Abstract Hedgehog signaling is central in embryonic development and tissue regeneration. Disruption of the pathway linked to genetic diseases cancer. Binding secreted ligand, Sonic hedgehog (ShhN) its receptor Patched (PTCH1) activates pathway. Here, we describe a 3.4 Å cryo-EM structure human PTCH1 bound ShhN C24II , modified ligand mimicking palmitoylated form. The membrane-embedded part surrounded by ten sterol molecules at inner outer lipid bilayer portion protein. annular sterols...
Abstract Robust oxygenic photosynthesis requires a suite of accessory factors to ensure efficient assembly and repair the oxygen-evolving photosystem two (PSII) complex. The highly conserved Ycf48 factor binds newly synthesized D1 reaction center polypeptide promotes initial steps PSII assembly, but its binding site is unclear. Here we have used cryo-electron microscopy determine structure cyanobacterial D1/D2 complex with attached. Ycf48, 7-bladed beta propeller, amino-acid residues that...
<p>Supplementary Figures S1-S4 - PDF file 482K, miR-214, miR-148b and ALCAM expression modulations (S1); is upregulated by miR-214 controls cell movement but not proliferation (S2); regulations (S3); ALCAM, TFAP2 in human melanoma samples (S4)</p>
<p>Supplementary Methods - PDF file 95K, Additional information regarding all reagents, antibodies, vectors and primers employed in this study, including experimental procedures</p>
<div>Abstract<p>Malignant melanoma is one of the most aggressive human cancers, but mechanisms governing its metastatic dissemination are not fully understood. Upregulation miR-214 and ALCAM loss TFAP2 expression have been implicated in this process, with a direct target miR-214. Here, we link as well identify core role for organizing metastasis. upregulated ALCAM, acting transcriptionally through also posttranscriptionally miR-148b (itself controlled by TFAP2), both negative...
<div>Abstract<p>Malignant melanoma is one of the most aggressive human cancers, but mechanisms governing its metastatic dissemination are not fully understood. Upregulation miR-214 and ALCAM loss TFAP2 expression have been implicated in this process, with a direct target miR-214. Here, we link as well identify core role for organizing metastasis. upregulated ALCAM, acting transcriptionally through also posttranscriptionally miR-148b (itself controlled by TFAP2), both negative...
<p>Supplementary Figures S1-S4 - PDF file 482K, miR-214, miR-148b and ALCAM expression modulations (S1); is upregulated by miR-214 controls cell movement but not proliferation (S2); regulations (S3); ALCAM, TFAP2 in human melanoma samples (S4)</p>
<p>Supplementary Methods - PDF file 95K, Additional information regarding all reagents, antibodies, vectors and primers employed in this study, including experimental procedures</p>
<p>Supplementary Figure and Table Legends - PDF file 74K, Legend for Supplemental S1 Figures S1-S4</p>
<p>Supplementary Figure and Table Legends - PDF file 74K, Legend for Supplemental S1 Figures S1-S4</p>