Aims: This study aimed to investigate the effect of probiotic bacterium Enterococcus faecium (E. faecium) on gene expression ion channels in colon cancer cells. Methods: The cytotoxic SW480 cell line was analyzed by MTT proliferation test using E. bacterial culture free supernatants. channel genes cells determined qRT PCR analysis. STRING analysis used reveal protein-protein interactions proteins. Bioinformatic healthy and patient data revealed with GEPIA platform. Results: line's viability...

Objective: The apoptotic effects of odoroside A on lung cancer cells were studied in our project. We also supported and extended experimentally-proven results via bioinformatics analysis human tissues. Materials Methods: In vitro studies conducted using the A549 cell line. Cell proliferation was evaluated through a CCK-8 assay. For gene expression analysis, qRT-PCR method used, while CASP3 protein levels detected Western blotting ELISA. Moreover, oxidant status determined by measuring TAS...

Precision diagnosis is of great importance and can be achievable through information processing sensors. A distyryl pyridinium BODIPY decorated with nitroreductase esterase enzyme responsive modules shown to display...

In the research, modulation of gene expression with a novel G-quadruplex stabiliser was analysed. Activation by removal bulky hypoxia-responsive substituent enhances stabilisation. Hypoxic MCF7 cells incubated displayed significant downregulation oncogenes c-myc, bcl-2, and hif-1α. This study presents first hypoxia-activatable stabilization transcriptional regulation.

Background/aim: Acrylamide is a cytotoxic, genotoxic and neurotoxic chemical for human.High level of acrylamide uptake causes effects, however the cellular damage mechanisms long-term low-dose are not fully known yet.The present study investigated cytotoxic effects on HEK293 cells.Materials methods: Genotoxic were examined by micronuclei formation assay its impact cell viability was measured MTT reduction assay.For studying genotoxicity determining source micronucleus, FISH (Fluorescent in...

The most common cancer seen in men worldwide is prostate cancer. It ranks second the deadliest types. Viburnum opulus a type of tree that produces small red fruits. Shell, fruit, flower and juice V. are widely used various fields traditional medicine. Studies have shown also has an anti-cancer effect. For this purpose, apoptotic effect was investigated LNCaP cell line. human cells were treated with extract 1-200 µg/mL concentration range. IC50 dose determined by viability test XTT....

Amaç: Makrofajlar bağışıklık sisteminin en önemli unsurlarından biridir ve inflamasyonda ikili rol oynamaktadır. Hem ortamda immün yanıtın oluşumunu hem de baskılanmasını düzenlemektedir. Kanser mikroçevresindeki bu sinyalleme, kanserin ilerlemesini etkiler. Bu kanseri teşvik eden veya ortadan kaldıran süreç makrofajlar tarafından yönetilmektedir. Proinflamatuar sitokin genlerinin ifadesindeki artış, ortamdaki tümör hücrelerinin sağlar. çalışmada Odoroside A'nın makrofaj dönüşümüne etkisinin...

Abstract Alzheimer's disease (AD) is an age‐related neurodegenerative disorder characterised by cognitive dysfunction, memory loss and mood changes. Hippocampal neurogenesis has been suggested to play a role in learning memory. Neurokinin 3 receptor (NK3R) shown be prevalent the hippocampus region. The aim of project was investigate hippocampal promnestic effects NK3R agonist administration amyloid beta‐induced AD rat model. Wistar albino rats were divided into control, Alzheimer, Alzheimer...

A cationic BODIPY-based G-quadruplex-selective stabiliser is developed and shown to decrease cancer cell migration-invasion up 90%. The expression of critical genes (HIF1α, VIM, CDH1) related metastasis modulated. reprograms hypoxia-adaptive metabolism an 1.82-fold increase in O2 consumption, enabling back-to-normal switching energy metabolism, observed. Stabilisers with a strong G-quadruplex affinity (0.38 μM Kd) significantly contribute small molecule anti-cancer approaches.

Sinapic acid (SA) has been shown to have anti-bacterial, anti-cancer, anti-inflammatory, cardioprotective, hepatoprotective, and neuroprotective effects in pre-clinical vitro investigations. Especially cancer studies, it that SA an anti-cancer effect. reduced proliferation induced apoptosis cells. In this study, the effect of on drug resistance hepatocellular carcinoma was investigated. For purpose, cells resistant sorafenib were obtained. According gene expression analysis performed cells,...

Endometrial carcinoma is one of the most common types cancer among women. The progression occurs via Epithelial- Mesenchymal Transition (EMT) pathway. Cells lose their epithelial properties and become mobile. For this reason, EMT process important step to be targeted in treatment. Oleandrin a cardiac glycoside its use limited due narrow therapeutic index. In study, we aimed evaluate effects lower level doses on endometrial carcinoma. was administrated Ishikawa adenocarcinoma cells at...

Preeclampsia occurs in the umbilical artery with proteinuria and edema accompanying pregnancy-induced hypertension. Vasoconstriction results severe complications such as intrauterine growth restriction, preterm birth, fetal death due to decreased uteroplacental blood flow. may cause perinatal mortality morbidity it is one of most important causes maternal mortality. The etiology pathogenesis preeclampsia, which present 7,18% all pregnancies, has not yet been elucidated. However, thought that...