A5101663803- Hemoglobin structure and function
- Spectroscopy and Quantum Chemical Studies
- Porphyrin and Phthalocyanine Chemistry
- Heme Oxygenase-1 and Carbon Monoxide
- Protein Structure and Dynamics
- Photochemistry and Electron Transfer Studies
- Photosynthetic Processes and Mechanisms
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Molecular spectroscopy and chirality
- Nitric Oxide and Endothelin Effects
- Photoreceptor and optogenetics research
- Nanoplatforms for cancer theranostics
- Advanced Chemical Physics Studies
- Magnetic and transport properties of perovskites and related materials
- Urinary Bladder and Prostate Research
- Advanced Condensed Matter Physics
- Physics of Superconductivity and Magnetism
- Mass Spectrometry Techniques and Applications
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Atomic and Molecular Physics
- Curcumin's Biomedical Applications
- Hemoglobinopathies and Related Disorders
- Iron-based superconductors research
Albert Einstein College of Medicine
2013-2025
Columbia University Irving Medical Center
1970-2022
Texas Health Dallas
2022
Binghamton University
2019-2022
Johns Hopkins Medicine
2020
New York Proton Center
2018-2020
Johns Hopkins University
2020
Indian Institute of Technology Madras
2020
University of Baltimore
2020
The Bronx Defenders
2020
Curcumin has been shown to have chondroprotective potential in vitro. However, its effect on disease and symptom modification osteoarthritis (OA) is largely unknown. This study aimed determine whether curcumin could slow progression of OA relieve OA-related pain a mouse model destabilization the medial meniscus (DMM). Expression selected cartilage degradative-associated genes was evaluated human primary chondrocytes treated with nanoparticles assayed by real-time PCR. The mice subjected DMM...
Abstract Several comorbidities of sickle cell disease (SCD) originate from red blood (RBC) instability, chronic inflammation, and oxidative stress. Development scalable, cost-effective therapeutics suitable for administration to prevent, attenuate, perhaps reverse the consequences RBC instability is needed. Curcumin has many these attributes as a safe compound with antisickling, antiinflammatory, antioxidant properties, but its translational potential been constrained due limited...
Staphylococcus aureus (SA) is a leading cause of diverse spectrum bacterial diseases, including abscesses. Nitric oxide (NO) critical component the natural host defense against pathogens such as SA, but its therapeutic applications have been limited by lack effective delivery options. We tested efficacy NO-releasing nanoparticle system (NO-np) in methicillin-resistant SA (MRSA) abscesses mice. The results show that NO-np exert antimicrobial activity MRSA vitro and Topical or intradermal...
Truncated hemoglobins (trHbs) are small hemoproteins forming a separate cluster within the hemoglobin superfamily; their functional roles in bacteria, plants, and unicellular eukaryotes marginally understood. Crystallographic investigations have shown that trHb fold (a two-on-two α-helical sandwich related to globin fold) hosts protein matrix tunnel system offering potential path for ligand diffusion heme distal site. The topology is conserved group I trHbs, although with modulation of its...
Inhomogeneous broadening of the 760-nanometer photoproduct band carboxymyoglobin at cryogenic temperatures has been demonstrated with a dynamic hole burning technique. Line-shape changes and frequency shifts in this spectral are generated by ligand recombination shown not to be result structural relaxation below 60 K. The observation exposes relation between disorder responsible for inhomogeneous well-known distributed rebinding kinetics. findings provide direct evidence functional relevance...
Acinetobacter baumannii (Ab) is a frequent cause of hospital acquired pneumonia and recently has increased in incidence as the causative agent severe disease troops wounded Afghanistan Iraq. Ab clinical isolates are frequently extremely resistant to antimicrobials, significantly complicating our capacity treat infections due this pathogen. Hence, development innovative therapeutics targeting mechanisms which bacteria unlikely evolve resistance urgently needed. We examined nitric oxide...
In this paper we report the results of an experimental study fluorescence excitation spectra and time-resolved emission Zn-tetraphenylporphyrin (ZnTPP) free-base tetraphenylporphyrin (H2TPP) seeded in pulsed supersonic expansions He. We have studied S0→S1 transition (the Q band) S0→S2 Soret, B ZnTPP, as well S0→S1x Qx band), S0→S1y Qy S0→S2x Bx H2TPP. Information was obtained on electronic energy levels, vibrational level structure, details low-frequency nuclear motion, some characteristics...
Truncated hemoglobin O (trHbO) is one of two trHbs in Mycobacterium tuberculosis. Remarkably, trHbO possesses novel distal residues, addition to the B10 tyrosine, that may be important ligand binding. These are CD1 tyrosine and G8 tryptophan. Here we investigate reactions mutants using stopped-flow spectrometry, flash photolysis, UV-enhanced resonance Raman spectroscopy. A biphasic kinetic behavior observed for combination dissociation O2 CO controlled by residues. The rate constants (<1.0...
Erythrocyte free hemoglobin (Hb) induces vasoconstriction due to nitric oxide (NO) scavenging, limiting the NO available for vascular smooth muscle. The central objective of this study was restore bioavailability using long-lived circulating NO-releasing nanoparticles (NO-np) reverse and hypertension induced by polymerized bovine Hb (PBH) scavenging. PBH (13 g/dl) infused in a volume equal 10% animal blood volume. Intravascular supplementation provided with an infusion NO-np (10 20 mg/kg...
Harnessing the impressive therapeutic potential of nitric oxide (NO) remains an ongoing challenge. This paper describes several current strategies both with respect to underlying chemistry and physics applications where they have shown promise. Included in this overview are molecular systems such as NONOates that release NO through chemical reactions delivery vehicles nanoparticles can generate, store, transport deliver related bioactive forms nitrosothiols. Although there has been much...
Curcumin is an effective and safe anticancer agent, also known to induce vasodilation, but its hydrophobicity limits clinical application. In this study, a simple emulsion method was developed prepare biodegradable poly (ethylene glycol)-poly (lactic acid) (PEG-PLA) nanospheres encapsulate curcumin improve solubility stability. The nanoparticle size around 150 nm with narrow distribution. Fluorescence microscopy showed that encapsulated PEG-PLA were taken up rapidly by Hela MDA-MB-231 cancer...
The sol−gel encapsulation process has been exploited in recent years for the immobilization of proteins to be used as biosensors. Sol−gels derived from tetramethyl orthosilicate provide a stable environment macromolecule combined with free flow small substrates protein's binding site. functionality number enzymes within solid matrix demonstrated. However, very little biophysical characterization encapsulated done. In this study, time-resolved fluorescence anisotropy was compare rotational...