A5000989306- Osteoarthritis Treatment and Mechanisms
- Temporomandibular Joint Disorders
- dental development and anomalies
- Bone health and treatments
- HIV/AIDS oral health manifestations
- Bone Metabolism and Diseases
- Inflammatory mediators and NSAID effects
- Proteoglycans and glycosaminoglycans research
- Orthodontics and Dentofacial Orthopedics
- Oral microbiology and periodontitis research
- Oral and gingival health research
- Laser Applications in Dentistry and Medicine
- Oropharyngeal Anatomy and Pathologies
- Dental Radiography and Imaging
- Bone and Dental Protein Studies
- Dental Research and COVID-19
- Estrogen and related hormone effects
- Bone health and osteoporosis research
- Connective tissue disorders research
- Dermatological and COVID-19 studies
- COVID-19 diagnosis using AI
- Oral and Maxillofacial Pathology
- Glycosylation and Glycoproteins Research
- Skin and Cellular Biology Research
- Cell Adhesion Molecules Research
Columbia University
2016-2025
Columbia University Irving Medical Center
2020-2023
UConn Health
2002-2018
Maulana Azad Medical College
2017
Primus Consulting (United States)
2017
Batra Hospital and Medical Research Centre
2014
University of Michigan–Ann Arbor
2010
National Institute of Dental and Craniofacial Research
2004-2007
National Institutes of Health
2005-2006
Because their etiologies and pathogenesis are poorly understood, temporomandibular joint (TMJ) diseases difficult to diagnose manage. All current approaches treatments of TMJ largely palliative. Definitive rational diagnoses or can only be achieved through a comprehensive understanding the etiologies, predisposing factors, diseases. While much work remains done in this field, novel findings biomedicine developments imaging computer technologies beginning provide us with vision future...
Extracellular matrix glycoproteins and proteoglycans bind a variety of growth factors cytokines thereby regulating assembly as well bone formation. However, little is known about the mechanisms by which extracellular molecules modulate osteogenic stem cells Using mice deficient in two members small leucine-rich proteoglycans, biglycan decorin, we uncovered role for these modulating formation from marrow stromal cells. Our studies showed that absence critical transforming factor-β...
Abstract Mechanical loading of bone may be transmitted to osteocytes and osteoblasts via shear stresses at cell surfaces generated by the flow interstitial fluid. The stimulated production prostaglandins, which mediates some effects mechanical on bone, is dependent inducible cyclo-oxygenase 2 (COX-2) in cells. We examined fluid stress (FSS) induction COX-2 gene expression immortalized MC3T3-E1 osteoblastic cells stably transfected with −371/+70 base pairs (bp) 5′-flanking DNA (Pluc371)...
A feedback loop between dysbiotic gut microbiota, increased translocation of microbial products such as lipopolysaccharide, and inflammation has been hypothesized to cause immune system dysfunction in early HIV infection. However, despite evidence a chronic inflammatory phenotype patients on antiretroviral therapy (ART), the role oral microbiota systemic activation relationship bacterial fungal diversity have not explored. Our study suggests crucial for communities long-term ART, expanding...
ABSTRACT Objective: To investigate the effect of externally applied cyclical (vibratory) forces on rate tooth movement, structural integrity periodontal ligament, and alveolar bone remodeling. Methods: Twenty-six female Sprague-Dawley rats (7 weeks old) were divided into four groups: CTRL (unloaded), VBO (molars receiving a vibratory stimulus only), TMO an orthodontic spring TMO+VB additional stimulus). In groups, rat first molars moved mesially for 2 using Nickel-Titanium coil delivering 25...
Abstract Background With effective antiretroviral therapy, people with HIV (PWH) are living longer and aging; the majority of PWH in United States now over age 50 women have gone through menopause transition. Menopause potentiates skeletal bone loss at spine, hip, radius PWH. The alveolar which surronds teeth is different than long bones because it derived from neural crest. However, few studies assessed oral health middle aged older HIV. Therefore, objective this study was to evaluate...
Abstract Background Several commercial programs incorporate artificial intelligence in diagnosis, but very few dental professionals have been surveyed regarding its acceptability and usability. Furthermore, explored how these advances might be incorporated into routine practice. Methods Our team developed implemented a deep learning (DL) model employing semantic segmentation neural networks object detection to precisely identify alveolar bone crestal levels (ABCLs) cemento-enamel junctions...
[Ca2+]e may be important in bone turnover. We found induces COX-2 transcription and PGE2 production primary calvarial osteoblasts through an ERK signaling pathway. Inhibition of inhibited the stimulation osteoblastic differentiation but not increase cell number. Hence, some effects on mediated by COX-2.Local changes extracellular calcium ([Ca2+]e) play role examined possibility that prostaglandins produced cyclo-oxygenase-2 (COX-2) could mediate osteoblasts.We induction expression...
The purpose of this study was to examine the effects forced mouth opening on murine mandibular condylar head remodeling. We hypothesized that would cause an anabolic response in cartilage. Six-week-old female C57BL/6 mice were divided into 3 groups: (1) control, (2) 0.25 N, and (3) 0.50 N opening. Gene expression, micro-CT, proliferation analyzed. 0.5 caused a significant increase mRNA expression Pthrp, Sox9, Collagen2a1, proliferation, trabecular spacing subchondral bone, whereas did not...
Abstract Temporomandibular joint (TMJ) disorders predominantly afflict women of childbearing age, suggesting a role for female hormones in the disease process. In long bones, estrogen acting via receptor beta (ERβ) inhibits axial skeletal growth mice. However, ERβ mandibular condyle is largely unknown. We hypothesize that ERβ-deficient mice will have increased condylar compared to wild-type (WT) This study examined 7-day-old, 49-day-old, and 120-day-old WT knockout (KO) There was significant...
To investigate the time course of osteocyte death in a mouse model orthodontic tooth movement (OTM) and its association to caspase-3 activation pathway osteoclast formation.Twenty-five male wild type CD-1 mice (8-12 weeks old) were loaded with an appliance. A spring delivering 10-12 g force was placed between right first molar incisor displace mesially. The contralateral unloaded sides served as control. animals equally divided into five different points: 6, 12, 24, 72 hours 7 days loading....
Temporomandibular joint degenerative disease (TMJ-DD) is a chronic form of TMJ disorder that specifically afflicts people over the age 40 and targets women at higher rate than men. Prevalence TMJ-DD in this population suggests estrogen loss plays role pathogenesis. Thus, goal present study was to determine on chondrogenesis homeostasis via receptor alpha (ERα) during growth maturity joint. Young mature WT ERαKO female mice were subjected ovariectomy procedures then given placebo or estradiol...
The temporomandibular joint (TMJ) within the craniofacial complex is unique. In humans, TMJ can become diseased resulting in severe and disabling pain. There are no cures for disease at this time. Animal models of scarce, but some exist, they described paper. We present greater detail one animal model that deficient two extracellular matrix (ECM) proteoglycans, biglycan (BGN) fibromodulin (FMOD). Doubly BGN/FMOD mice develop premature osteoarthritis (OA). order to explore mechanistic basis...
Olson C., Uribe F., Kalajzic Z., Utreja A., Nanda R., Rowe D., Wadhwa S. Orthodontic tooth movement causes decreased promoter expression of collagen type 1, bone sialoprotein and alpha‐smooth muscle actin in the periodontal ligament. Orthod Craniofac Res 2012; 15 :52–61 © John Wiley & Sons A/S Structured Abstract Objective – To evaluate effects orthodontic on 1 (3.6Col1), (BSP) (αSMA) ligament (PDL) using transgenic mice containing transgenes these promoters fused to green fluorescent...
Abstract Mechanical loading of bone generates fluid flow within the mineralized matrix that exerts shear stress (FSS) on cells. We examined effects FSS receptor activator nuclear factor κ B ligand (RANKL), a critical for osteoclast formation. Primary murine osteoblasts were subjected to pulsatile (5 Hz, 10 dynes/cm 2 ) 1 h and then returned static culture varying times (post‐FSS). Protein levels measured by Western analysis mRNA Northern analysis, RT‐PCR quantitative PCR. There 20‐ 40‐fold...
<i>Objectives:</i> The mandibular condylar cartilage is a heterogeneous tissue containing cells at various stages of chondrocyte maturation organized into 4 zones: superficial, polymorphic, flattened, and hypertrophic. goal this study was to use transgenic mice markers fused fluorescent protein transgenes isolate characterize homogenous cell populations the cartilage. <i>Methods:</i> Fluorescent reporter expression in 3.6-kb fragment rat collagen type 1 promoter...