A5061358029- Ginseng Biological Effects and Applications
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Pharmacogenetics and Drug Metabolism
- Estrogen and related hormone effects
- Mosquito-borne diseases and control
- Neuroscience and Neuropharmacology Research
- Lipid metabolism and biosynthesis
- Thyroid Disorders and Treatments
- Traditional Chinese Medicine Analysis
- Sphingolipid Metabolism and Signaling
- Computational Drug Discovery Methods
- Virology and Viral Diseases
- Nanoparticle-Based Drug Delivery
- Hormonal Regulation and Hypertension
- Pharmacological Effects and Toxicity Studies
- Influenza Virus Research Studies
- Fungal Infections and Studies
- Heme Oxygenase-1 and Carbon Monoxide
- Biosimilars and Bioanalytical Methods
- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- Metabolism, Diabetes, and Cancer
- Nitric Oxide and Endothelin Effects
- Antifungal resistance and susceptibility
University of Arizona
2019-2024
University of Montana
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2017
Yantai University
2012-2014
Mayo Clinic in Arizona
2009
Abstract Cholesterol (Chol) fortifies packing and reduces fluidity permeability of the lipid bilayer in vesicles (liposomes)-mediated drug delivery. However, under physiological environment, Chol is rapidly extracted from by biomembranes, which jeopardizes membrane stability results premature leakage for delivered payloads, yielding suboptimal clinic efficacy. Herein, we report a Chol-modified sphingomyelin (SM) via covalently conjugating to SM (SM-Chol), retains condensing ability Chol....
SARS-CoV-2 has caused a global pandemic with significant humanity and economic loss since 2020. Currently, only limited options are available to treat infections for vulnerable populations. In this study, we report universal fluorescence polarization (FP)-based high throughput screening (HTS) assay SAM-dependent viral methyltransferases (MTases), using fluorescent SAM-analogue, FL-NAH. We performed the against reference MTase, NSP14, an essential enzyme methylate N7 position of 5'-RNA...
Stereoselectivity has been proved to be tightly related drug action including pharmacodynamics and pharmacokinetics. (20S,24R)-epoxy-dammarane-3,12,25-triol (24R-epimer) (20S,24S)-epoxy-dammarane-3,12,25-triol (24S-epimer), a pair of 20(S)-protopanaxadiol (PPD) ocotillol type epimers, were the main metabolites PPD. Previous studies have shown that 24R-epimer 24S-epimer had stereoselectivity in pharmacological In present study, aim was further study pharmacokinetic characteristics both...
Zika virus (ZIKV) causes significant human diseases without specific therapy. Previously we found erythrosin B, an FDA-approved food additive, inhibited viral NS2B-NS3 interactions, leading to inhibition of ZIKV infection in cell culture. In this study, performed pharmacokinetic and vivo studies demonstrate the efficacy B against 3D mini-brain organoid mouse models. Our results showed that is very effective abolishing replication model. Although pharmacokinetics indicated had a low...
Seasonal influenza A virus (IAV) infections are among the most important global health problems. FDA-approved antiviral therapies against IAV include neuraminidase inhibitors, M2 and polymerase inhibitor baloxavir. Resistance adamantanes (amantadine rimantadine) is widespread as virtually all strains currently circulating in human population resistant to through acquisition of S31N mutation. The inhibitor-resistant also contain M2-S31N mutant, suggesting a high-profile drug target. Here we...
Abstract The sex-specific prevalence of adrenal diseases has been known for a long time. However, the reason high these in females is not completely understood. Mouse studies have shown that adult gland sexually dimorphic at different levels such as transcriptome, histology, and cell renewal. Here we used RNA-seq to show prepubertal mice, male female glands were only but also responded differently same external stimulus. We previously reported thyroid hormone receptor β1 (TRβ1) mainly...
Drug resistance is a significant concern in the treatment of diseases, including cryptococcosis caused by Cryptococcus neoformans (Cne) and gattii (Cga). Alternative drug targets are necessary to overcome before it attains critical stage. Splicing inteins from pro-protein precursors crucial for activities essential proteins hosting intein elements many organisms, human pathogens such as Cne Cga. Through high-throughput screening, we identified calcimycin (CMN) potent Prp8 splicing inhibitor...
Abstract (20 S ,24 )‐epoxy‐dammarane‐3,12,25‐triol (24 ‐epimer) and R )‐epoxy‐ dammarane‐3,12,25‐triol ‐epimer), a pair of ocotillol type epimers, were identified as the main metabolites 20( )‐protopanaxadiol (PPD). The aim this study was to systematically investigate formation metabolism epimers in vivo vitro elucidate isoforms cytochrome P450 enzymes responsible for stereoselective both epimers. result showed that 24 ‐epimer more predominant ingredient rat plasma after oral administration...
10-Chloromethyl-11-demethyl-12-oxo-calanolide (F18), an analogue of calanolide A, is a novel potent nonnucleoside reverse transcriptase inhibitor against HIV-1. Here, we report the metabolic profile and results associated biochemical studies F18 in vitro vivo. The metabolites were identified based on liquid chromatography-electrospray ionization mass spectrometry and/or nuclear magnetic resonance. Twenty-three observed liver microsomes vitro. metabolism involved 4-propyl chain oxidation,...
Aging increases susceptibility to lung disease, but the topic is understudied, especially in relation environmental exposures with bulk of rodent studies using young adults. This study aims define pulmonary toxicity naphthalene (NA) and impacts a dietary antioxidant, ergothioneine (ET), liver lungs middle-aged mice. NA causes well-characterized pattern conducting airway epithelial injury adult mice, NA's has not been characterized aged 1-1.5 years. ET antioxidant that synthesized by bacteria...
Abstract #19 Background: Tamoxifen (TAM) is a standard endocrine therapy for the treatment of women with estrogen receptor (ER) positive breast cancer. TAM activated by cytochrome P450 2D6 enzyme system into two potent and active metabolites, 4-hydroxytamoxifen (4HT) 4-hydroxy-N-desmethyl-tamoxifen (endoxifen). While human concentrations 4HT are negligible vary little in plasma (5-10 nM), endoxifen widely (10-180 nm), genetically impaired CYP2D6 metabolism have significantly reduced levels...
Gindenosides are the active ingredients of Panax ginseng. 20 (S) -protopanaxadiolocotillol type epimers main metabolites -protopanaxadiol. The previous studies showed that there stereoselectivity difference in pharmacodynamics and pharmacokinetics between 24R-epimer 24S-epimer. purpose this study was to explore excretion bile, feces urine rat. Liquid chromatography tandem mass spectrometry method has been performed for determination 24S-epimer urine. or intragastric administered rats at a...
Arsenic exposure has been associated with the risks of various diseases, including cancers and metabolic diseases. The aim this study was to examine effects arsenic via drinking water on expression heme oxygenase-1 (HO-1), a major responsive gene arsenic-induced oxidative stress, in mouse intestinal epithelial cells which is first site for ingested arsenic, liver, known target toxicity. HO-1 determined at mRNA, protein, or enzymic activity levels mice exposed sodium arsenite through water,...
A sensitive and specific UPLC-MS/MS method was developed validated for the simultaneous determination of 2-amino-2-(2-(4'-(2-propyloxazol-4-yl)-[1,1'-biphenyl]-4-yl)ethyl)propane-1,3-diol (SYL930), phosphorylated metabolite (SYL930-P) hydroxylated (SYL930-M) in dog blood using SYL927 SYL927-P, analogues SYL930, as internal standards. Analytes were extracted with protein precipitation followed by chromatographic separation on a ZorbaxSB-C18 column (3.5 μm, 2.1 × 100 mm) gradient elution...
<b>Abstract ID 26143</b> <b>Poster Board 259</b> Human serum albumin (SA) is the most abundant protein in blood, typically at 30–50 mg/ml and a commonly used for studying human exposure to xenobiotic compounds. A SA transgenic mouse model was previously reported (PMC4868631), which endogenous predominates competes with adduction reactive metabolites. The aim of this study establish, via crossbreeding SA-null mouse, characterize SA-humanized model, where only human, but not expressed....
Environmental exposure to arsenic is associated with increased incidences of multiple adverse health consequences in humans, posing a significant risk people living near contaminated sites. For example, populations residing mining sites may be exposed mine tailings through the inhalation dust particles or ingestion water food, as contaminants become bio‐accessible. Previous studies rodents reported ability short‐term exposures at high doses increase decrease expression several xenobiotic...