A5043035042- Advanced Fluorescence Microscopy Techniques
- DNA and Nucleic Acid Chemistry
- DNA Repair Mechanisms
- HIV Research and Treatment
- Advanced Electron Microscopy Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Near-Field Optical Microscopy
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Chromatin Dynamics
- Herpesvirus Infections and Treatments
- HIV/AIDS drug development and treatment
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Single-cell and spatial transcriptomics
- Lanthanide and Transition Metal Complexes
- Nanopore and Nanochannel Transport Studies
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Surface Chemistry and Catalysis
- Enzyme Structure and Function
- RNA modifications and cancer
- Bacteriophages and microbial interactions
Delft University of Technology
2024-2025
Freie Universität Berlin
2016-2021
University of Groningen
2013-2016
Institute of Molecular Biology and Biophysics
2014
Northeastern University
2010-2013
École Normale Supérieure de Lyon
2011
Vrije Universiteit Amsterdam
2009-2010
Herpes simplex virus type 1 (HSV1) capsids undergo extensive structural changes during maturation and DNA packaging. As a result, they become more stable competent for nuclear egress. To further elucidate this stabilization process, we used biochemical nanoindentation approaches to analyze the mechanical properties of scaffold-containing (B), empty (A), DNA-containing (C) capsids. Atomic force microscopy experiments revealed that A C were mechanically indistinguishable, indicating presence...
Significance The DNA replication machinery is a multiprotein assembly that responsible for rapid and faithful duplication of the genome. Based on biochemical, structural, genetic studies, we have obtained very detailed picture its different enzymatic activities underlie unwinding parental duplex incorporation nucleotides into daughter DNA. However, little known about dynamic changes in composition complex during replication. Here, use single-molecule fluorescence methods to visualize...
Single molecule localization-based approaches to super-resolution microscopy (SMLM) create images that resolve features smaller than the diffraction limit of light by rendering them from sequentially measured positions thousands individual molecules. New SMLM based on transient binding very bright dyes via DNA–DNA interaction (DNA-PAINT) allow resolution only a few nanometers apart in vitro. This imaging cellular structures requires specific association their targets, which results an...
Super-resolution microscopy facilitates the visualization of cellular structures at a resolution approaching molecular level. Especially, super-resolution techniques based on localization single molecules have relatively modest instrument requirements and are thus good candidates for adoption in bioimaging. However, their low-throughput nature hampers applicability biomolecular research screening. Here, we propose workflow more efficient data collection, starting with scanning large areas...
Abstract Single molecule localization-based approaches to superresolution microscopy (SMLM) create images that resolve features smaller than the diffraction limit of light by rendering them from sequentially measured positions thousands individual molecules. New SMLM based on transient binding very bright dyes via DNA-DNA interaction (DNA-PAINT) allow resolution only a few nanometers apart in vitro . This imaging cellular structures requires specific association their targets, which results...
Reverse transcription in retroviruses and retrotransposons requires nucleic acid chaperones, which drive the rearrangement of conformation. The chaperone properties human immunodeficiency virus type-1 (HIV-1) nucleocapsid (NC) protein have been extensively studied, aggregation, duplex destabilization rapid binding kinetics identified as major components its activity. However, other proteins, such retrotransposon Ty3 NC, a likely ancestor HIV-1 are not well understood. In addition, it is...
ABSTRACT Super-resolution microscopy facilitates the visualization of cellular structures at resolutions approaching molecular level. Especially, super-resolution techniques based on localization single-molecules have relatively modest instrument requirements and are thus good candidates for adoption in bioimaging. However, their low throughput nature hampers applicability biomolecular research screening. Here, we propose a workflow more efficient data collection, starting with scanning...
Abstract DNA point accumulation in nanoscale topography (DNA-PAINT) advances super-resolution microscopy with superior resolution and multiplexing capabilities. However, cellular may interfere this single-molecule localization technique based on DNA-DNA hybridization. Here, we introduce left-handed (L-DNA) oligomers that do not hybridize to naturally present R-DNA demonstrate L-DNA PAINT has the same specificity capability as PAINT, but greatly improves specific visualization of nuclear...