A5044085733- Plant-Microbe Interactions and Immunity
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Fungal Plant Pathogen Control
- Agriculture, Plant Science, Crop Management
- Plant Pathogens and Resistance
- Legume Nitrogen Fixing Symbiosis
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Genomics, phytochemicals, and oxidative stress
- Agronomic Practices and Intercropping Systems
- Garlic and Onion Studies
- Plant nutrient uptake and metabolism
- Nematode management and characterization studies
- Plant pathogens and resistance mechanisms
- Polysaccharides and Plant Cell Walls
- Mycorrhizal Fungi and Plant Interactions
- Plant and animal studies
- Composting and Vermicomposting Techniques
- Fungal Infections and Studies
- Moringa oleifera research and applications
- Plant Disease Management Techniques
- Plant Physiology and Cultivation Studies
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy and Chemometric Analyses
Université d'Angers
2006-2024
L'Institut Agro
2022-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2022-2024
Institut de recherche en horticulture et semences
2012-2022
Institut Agro Rennes-Angers
2013-2022
Natural Resources Canada
2012
Canadian Forest Service
2012
Biologie des Plantes et Innovation
2011
Laboratoire Physiologie Cellulaire & Végétale
2008-2010
Centre National de la Recherche Scientifique
2007
Glucosinolates are brassicaceous secondary metabolites that have long been considered as chemical shields against pathogen invasion. Isothiocyanates, glucosinolate-breakdown products negative effects on the growth of various fungal species. We explored mechanism by which isothiocyanates could cause cell death using Alternaria brassicicola, a specialist Brassica pathogens, model organism. Exposure fungus to led decreased oxygen consumption rate, intracellular accumulation reactive species and...
Camalexin, the characteristic phytoalexin of Arabidopsis thaliana, inhibits growth fungal necrotroph Alternaria brassicicola. This plant metabolite probably exerts its antifungal toxicity by causing cell membrane damage. Here we observed that activation a cellular response to this damage requires wall integrity (CWI) and high osmolarity glycerol (HOG) pathways. Camalexin was found activate both AbHog1 AbSlt2 MAP kinases, latter abrogated in deficient strain. Mutant strains lacking functional...
Seeds are involved in the vertical transmission of microorganisms from one plant generation to another and consequently act as reservoirs for microbiota. However, little is known about structure seed-associated microbial assemblages regulators assemblage structure. In this work, we have assessed response Raphanus sativus invading phytopathogenic agents, bacterial strain Xanthomonas campestris pv. ( Xcc ) 8004 fungal Alternaria brassicicola Abra43. According indicators (16S rRNA gene gyrB...
A second mannitol transporter, AgMaT2, was identified in celery (Apium graveolens L. var. dulce), a species that synthesizes and transports mannitol. This transporter successfully expressed two different heterologous expression systems: baker's yeast (Saccharomyces cerevisiae) cells tobacco (Nicotiana tabacum) plants (a non-mannitol-producing species). Data indicated AgMaT2 works as an H+/mannitol cotransporter with weak selectivity toward other polyol molecules. When tobacco, decreased the...
We have shown that the plant pathogen Alternaria brassicicola exhibited very high susceptibility to ambruticin VS4 and a lesser extent phenylpyrrole fungicide fludioxonil. These compounds are both derived from natural bacterial metabolites with antifungal properties thought exert their toxicity by interfering osmoregulation in filamentous fungi. Disruption of osmosensor group III histidine kinase gene AbNIK1 (for A. brassicola NIK1) resulted levels resistance fludioxonil, while mutant...
Abstract Background Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning or plant genetic factors that impact seed and mechanisms underlying this key biological trait have yet to be clarified. Such lack available data could probably explained by absence suitable model pathosystem study plant-fungus interactions during reproductive phase. Results Here we report on setting up new facilitate transmission....
ABSTRACT Alternaria brassicicola causes dark spot (or black spot) disease, which is one of the most common and destructive fungal diseases Brassicaceae spp. worldwide. Here, we report draft genome sequence strain Abra43. The assembly comprises 29 scaffolds, with an N 50 value 2.1 Mb. assembled was 31,036,461 bp in length, a G+C content 50.85%.
Image analysis is increasingly used in plant phenotyping. Among the various imaging techniques that can be phenotyping, chlorophyll fluorescence allows of impact biotic or abiotic stresses on leaves. Numerous parameters may measured calculated, but only a few produce contrast given condition. Therefore, automated procedures help screening image datasets are needed, especially perspective high-throughput
In this study, the roles of fungal dehydrin-like proteins in pathogenicity and protection against environmental stresses were investigated necrotrophic seed-borne fungus Alternaria brassicicola. Three (called AbDhn1, AbDhn2 AbDhn3), harbouring asparagine-proline-arginine (DPR) signature pattern sharing characteristic features proteins, identified A. brassicicola genome. The expression these genes was induced response to various found be regulated by AbHog1 mitogen-activated protein kinase...
The transmission of seed-borne pathogens by the germinating seed is responsible for major crop diseases. immune responses facing biotic invaders are poorly documented so far.
Summary Pinewood nematode, Bursaphelenchus xylophilus, is an inhabitant of native pine species North America, where its presence in trees non‐pathogenic. By contrast, the introduction this nematode to forests overseas has devastated some stands and recognized as a pest phytosanitary concern by countries' National Plant Protection Organizations. The ability detect B. xylophilus internationally traded wood products crucial reduce spread organism. Current molecular techniques for detection rely...
This study describes the gel-free phosphoproteomic analysis of phytopathogenic fungi Alternaria brassicicola and Botrytis cinerea grown in vitro under nonlimiting conditions. Using a combination strong cation exchange IMAC prior to LC-MS, we identified over 1350 phosphopeptides per fungus representing 800 phosphoproteins. The preferred phosphorylation sites were found on serine (>80%) threonine (>15%), whereas phosphorylated tyrosine residues at less than 1% A. slightly higher ratio B....
Alternaria brassicicola causes black spot disease in Brassicaceae . During host infection, this necrotrophic fungus is exposed to various antimicrobial compounds, such as the phytoalexin brassinin which produced by many cultivated Brassica species. To investigate cellular mechanisms compound toxicity and corresponding fungal adaptive strategies, we first analyzed transcriptional responses short-term exposure then used additional functional approaches. This study supports hypothesis that...
The impact of the form nitrogen (N) source (nitrate versus ammonium) on susceptibility to Alternaria brassicicola, a necrotrophic fungus, has been examined in Arabidopsis thaliana at rosette stage. Nitrate nutrition was found increase fungal lesions considerably. There similar induction defence gene expression following infection under both N nutritions, except for phytoalexin deficient 3 gene, which overexpressed with nitrate. also led greater nitric oxide production occurring planta during...
Little is known about the effect of nitrogen nutrition on seedling susceptibility to seed-borne pathogens. We have previously shown that seedlings grown under high nitrate (5 mM) conditions are less susceptible than those low (0.1 and ammonium in Arabidopsis-Alternaria brassicicola pathosystem. However, it not how metabolism modulated by nutrition, nor what its response pathogen infection. Here, we addressed this question using same pathosystem nutritive conditions, examining germination...
The process of infection carrots by Pythium violae and ultimum, two causes cavity spot, is described. first species limited root necrosis, the second progressive rot. Colonization both was intracellular within tissues. Modes cell wall degradation were studied staining (PATAg test) labeling techniques. Pectins labeled with monoclonal antibodies cellulose an exoglucanase-gold complex. Cell polysaccharides degraded differently species. responsible for degradations, which could be noticeable,...
Alternaria brassicicola is a necrotrophic fungus causing black spot disease and an economically important seed-borne pathogen of cultivated brassicas. Seed transmission crucial component its parasitic cycle as it promotes long-term survival dispersal. Recent studies, conducted with the Arabidopsis thaliana/A. pathosystem, showed that level susceptibility to water stress strongly influenced seed ability. In this study, we gained further insights into mechanisms involved in infection process...