A5008794853- Protein Structure and Dynamics
- Enzyme Structure and Function
- Mass Spectrometry Techniques and Applications
- Hemoglobin structure and function
Donostia International Physics Center
2022-2025
University of the Basque Country
2024-2025
Material Physics Center
2022-2024
The synthesis of ultrasmall poly-L-lysine (PLL) nanoparticles remains challenging using conventional nanogel preparation methods. This study investigates how the conformation PLL precursor chains influences resulting nanoparticle microstructure when crosslinking is performed under dilute conditions. Structural characterization by small-angle neutron scattering (SANS), complemented dynamic light (DLS) and proton nuclear magnetic resonance ($^1$H-NMR), reveals that pre-collapsing...
Protein single-chain nanoparticles can outperform synthetic in biomedical applications due to enhanced biocompatibility. Compared (co)polymers, the chemical complexity of proteins challenges chain conformation control. Here, we investigate impact precursor bovine serum albumin (BSA) on nanoparticle structure after intramolecular cross-linking. We explore urea concentration (denaturant), pH, salt, cross-linker length, and concentration. Small-angle neutron scattering dynamic light experiments...
The main challenge for the preparation of protein single-chain nanoparticles (SCNPs) is natural complexity these macromolecules. Herein, we report suitable conditions to produce "neat" bovine serum albumin (BSA) from partially denatured BSA, which involves denaturation in urea and intramolecular cross-linking below overlap concentration. We use two disuccinimide ester linkers containing three six methylene spacer groups: disuccinimidyl glutarate (DSG) suberate (DSS), respectively....