A5027062225- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- RNA regulation and disease
- RNA Research and Splicing
- COVID-19 Pandemic Impacts
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- Parasites and Host Interactions
- Identification and Quantification in Food
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- COVID-19 diagnosis using AI
- COVID-19 epidemiological studies
- Amoebic Infections and Treatments
- Parasitic Infections and Diagnostics
Korea Advanced Institute of Science and Technology
2023-2024
Atma Jaya Catholic University of Indonesia
2021-2024
University of Indonesia
2020
Saliva as a sample matrix has been an attractive alternative for the detection of SARS-CoV-2. However, due to potential variability in collection and processing steps, evaluating proposed workflow amongst local population is recommended. Here, we aim validate treatment human saliva direct specimen RT-qPCR-based SARS-CoV-2 Indonesia. We demonstrated that target genes were detected specimens remained stable five days either refrigerated or stored at room temperature. The method described this...
The testing capacity of the laboratory is paramount for better control pandemic caused by SARS-CoV-2. pooling method promising to increase capacity, and use direct NAAT-based detection SARS-CoV-2 on a non-invasive specimen such as saliva will ultimately accelerate capacity. This study aims validate pooling-of-four quadruple using RNA-extraction-free specimens. In addition, we intend investigate preferable stage pooling, including pre- or post-heating. compatibility this approach was also...
Since January 2022, offline learning in the COVID-19 pandemic era has been going on for nearly a year. The government is attempting to revive education sector, including universities. Nonetheless, this policy may result formation of new SARS-CoV-2 infection cluster campuses. One prevention measurements conducting examinations. To support effort, infectious disease research team at Atma Jaya Catholic University Indonesia (AJCUI) examined by saliva specimens using RT-qPCR method, along with...
Abstract Introns are found in all eukaryotes and one of the defining characteristics eukaryotes. After transcription, pre-mRNAs undergo splicing, introns excised lariat structure. Since degraded quickly after debranching, they have been considered as byproducts gene expression, their post-splicing role cells remains unclear. Here, we find that inhibition intron debranching process by depleting a enzyme, DBR1, results hyperactivation protein kinase R (PKR) due to accumulation lariat-derived...
Intestinal parasitic infections (IPIs), caused by helminths and/or protozoa, continue to be a significant public health concern in Indonesia. Water access, sanitation, and hygiene practices (WASH) are influential factors for IPIs, especially among children. The aim of this study was investigate the association between WASH IPIs school-aged A cross-sectional involving 338 school-age children an urban slum area North Jakarta, Indonesia, conducted using stool specimens subjected microscopic...
Abstract Mislabeling of fish fillet product is one the key issues in food safety and sustainability. Species identification an important step traceability DNA barcoding has been proved as a standard method. Fourty seven 47 products were collected from modern traditional markets Jabodetabek area. was used to analyze compliance label. This research also highlighted that near threatened (NT), Vulnerable (VU), endangered (EN), critically (CR) species considered be facing high risk extinction...
Abstract Saliva as a sample matrix has been an attractive alternative for the detection of SARS-CoV-2. However, due to potential variability in collection and processing steps, evaluating proposed workflow amongst local population is recommended. Here, we aim validate treatment human saliva direct specimen RT-qPCR-based SARS-CoV-2 Indonesia. We demonstrated that target genes were detected specimens remained stable five days refrigerated or room temperature storage. The method described this...
Mitochondria are essential regulators of cellular processes, including innate immunity. They generate long double-stranded RNAs (mt-dsRNAs) and release them to the cytosol trigger immune response under pathological stress conditions. Yet, regulation these immunogenic remains largely unknown. Here, we employ CRISPR screening on RNA-binding proteins residing in mitochondria identify NOP2/Sun RNA methyltransferase 4 (NSUN4) as a key regulator mt-dsRNA expression. We find that NSUN4 directly...
Abstract Atma Jaya Catholic University of Indonesia (AJCUI) COVID-19 Laboratory has become a reference testing site, which contributed to prevent the SARS-CoV-2 transmission in Indonesia. Through LSSR and CARE, Indonesian government implemented moderate yet arguably successful policies combat this pandemic. This study aims assess strengthen public health management while enhancing our understanding dynamics COVID-19. We analyzed correlation between enforced controlling from July...