A5046239368- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- RNA regulation and disease
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Genetic Neurodegenerative Diseases
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Lipoproteins and Cardiovascular Health
- Neurological Disorders and Treatments
- Regulation of Appetite and Obesity
- Microbial Community Ecology and Physiology
- Acute Ischemic Stroke Management
- Molecular Biology Techniques and Applications
- Galectins and Cancer Biology
- Nutrition and Health in Aging
- Cancer, Hypoxia, and Metabolism
- MRI in cancer diagnosis
- Protein purification and stability
- Biopolymer Synthesis and Applications
- Traumatic Brain Injury and Neurovascular Disturbances
- Bacteriophages and microbial interactions
- Cancer, Lipids, and Metabolism
- Stress Responses and Cortisol
- Muscle Physiology and Disorders
The University of Tokyo
2016-2017
Kagoshima University
1988-1993
Efficient production of bispecific antibodies (BsAbs) in single mammalian cells is essential for basic research and industrial manufacturing. However, preventing unwanted pairing heavy chains (HCs) light (LCs) a challenging task. To address this, we created an engineering technology preferential cognate HC/LC HC/HC paring called FAST-Ig (Four-chain Assembly by electrostatic Steering Technology – Immunoglobulin), applied it to NXT007, BsAb the treatment hemophilia A. We introduced charged...
Abstract: We studied the serum lipoprotein and apolipoprotein profiles in 44 patients with sporadic late‐onset Alzheimer's dementia 43 vascular dementia. The levels of high‐density (HDL) cholesterol were lower both patient groups than a control group. Apolipoprotein A I II have decreased groups, especially HDL‐cholesterol correlated positively level I, but not II. ratios I/A increased groups. disproportionally may involve pathological process senile
Summary The RNA-guided endonuclease Cpf1 is a promising tool for genome editing in eukaryotic cells 1-5 . Compared to other platforms, offers distinct advantages, such as the ability easily target multiple genes simultaneously 3 , well low rates of off-target activity 4, 5 However, Acidaminococcus sp. BV3L6 (AsCpf1), which has been successfully harnessed editing, can only robustly cleave sites preceded by TTTV protospacer adjacent motif (PAM), may limit its practical utility. To address this...
The CRISPR-associated endonuclease Cas9 can be targeted to specific genomic loci by single guide RNAs (sgRNAs).We first solved the crystal structure of Streptococcus pyrogenes (SpCas9) in complex with sgRNA and its target DNA at 2.5 A resolution.The revealed a bilobed architecture consisting recognition nuclease lobes (Rec Nuc lobes, respectively), accommodating sgRNA:DNA heteroduplex positively-charged groove their interface.While Rec lobe is essential for binding DNA, contains HNH RuvC...
すでにわれわれはRISA体外計測法を用い, 頭部血量と平均脳循環時間測定値より頭部血流量を算出し, その測定法および成績について報告した。頭部血量の測定誤差は現在ほぼ7.5%以内にとどめ得るが, Loveらも指摘するごとく平均脳循環時間については方法いかんにより誤差介入の余地が大きく, 脳血流量計算値に及ぼす意義が大きい。よって, 今回かかる点を検討し頭部および頸部より同時記録した血管放射図の対比より, 頸動脈→頸静脈球循環時間 (T) , 頸動脈→脳底動脈環循環時間 (ta) 静脈洞連合→頸静脈循環時間 (tv) を求め, これらの値より平均脳循環時間 (t) を求めた。本法によりtは正常者で平均6.7秒で再現性にすぐれ, 脳血管障害例では延長が明らかであった。
Immunohistochemical examinations using monoclonal antibody against subunit IV of cytochrome c oxidase were carried out in the muscle patients with various mitochondrial myopathies having histochemical partial deficiency activity. The ragged red fibers showed positive reaction for even deficient fibers. However, normal histological appearance, immunohistochemical varied. While all reactivity had no detectable activity, protein was not cause