A5067572688- Cell Image Analysis Techniques
- Microfluidic and Bio-sensing Technologies
- Single-cell and spatial transcriptomics
- Plasmonic and Surface Plasmon Research
- Advanced Fluorescence Microscopy Techniques
- Digital Holography and Microscopy
- Extracellular vesicles in disease
- Photonic and Optical Devices
- Nanopore and Nanochannel Transport Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Microfluidic and Capillary Electrophoresis Applications
- Random lasers and scattering media
- CAR-T cell therapy research
- 3D Printing in Biomedical Research
- Photoacoustic and Ultrasonic Imaging
- Diffusion and Search Dynamics
- CRISPR and Genetic Engineering
- Pickering emulsions and particle stabilization
- Chronic Myeloid Leukemia Treatments
- Thermography and Photoacoustic Techniques
- Electrowetting and Microfluidic Technologies
- Digital Imaging for Blood Diseases
The University of Tokyo
2016-2025
Think Surgical (United States)
2017-2024
Japan Science and Technology Agency
2018-2021
Judd Systems Technologies (United States)
2017
University of California, Berkeley
2011-2015
Lawrence Berkeley National Laboratory
2014
U.S. National Science Foundation
2013
Berkeley College
2013
Laboratory for Integrated Micro-Mechatronic Systems
2008
Tokyo University of Science
2008
Ghost imaging is a technique used to produce an object's image without using spatially resolving detector. Here we develop term "ghost cytometry," image-free ultrafast fluorescence "imaging" cytometry based on single-pixel Spatial information obtained from the motion of cells relative static randomly patterned optical structure compressively converted into signals that arrive sequentially at Combinatorial use temporal waveform with intensity distribution random pattern allows us...
Gently down the stream: A microfluidic technique uses a continuous fluid stream to generate monodisperse unilamellar phospholipid vesicles from single bilayer (see picture). Since are robust and efficiently encapsulate high concentrations of various molecules, they useful as delivery vehicles model cellular systems.
Pluripotent stem cell-based therapy for retinal degenerative diseases is a promising approach to restoring visual function. A clinical study using organoid (RO) sheets was recently conducted in patients with retinitis pigmentosa. However, the graft preparation currently requires advanced skills identify and excise suitable segments from transplantable area of limited number ROs. This remains challenge consistent implementations. Herein, we enabled enrichment wild-type (non-reporter)...
Recent advancements in image-based pooled CRISPR screening have facilitated the mapping of diverse genotype-phenotype associations within mammalian cells. However, rapid enrichment cells based on morphological information continues to pose a challenge, constraining capacity for large-scale gene perturbation across high-content cellular phenotypes. In this study, we demonstrate applicability multimodal ghost cytometry-based cell sorting, including both fluorescent and label-free phenotypes,...
We demonstrate an all-dielectric quantum electrodynamical nanowire-slab system with a single emitter that concentrates the extremely intense light at scale of 10 × 75 nm2. The dot exhibits record high 31-fold spontaneous decay rate enhancement, its optical saturation and blinking are strongly suppressed, 80% emission couples into waveguide mode.
We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel the axis of an objective lens without scanning. APOM combined with simultaneously provides two orthogonal images 3D sample. More importantly, uses only single near sample achieve selective-plane illumination as we demonstrated by three-dimensional (3D) imaging fluorescent pollens and brain slices. This technique allows fast, high-contrast...
The large-scale multiparametric analysis of individual nanoparticles is increasingly vital in the diverse fields biology, medicine, and materials science. However, current methods struggle with tradeoff between measurement scalability sensitivity, especially when identifying rare heterogeneous mixtures. By developing combining an unsupervised deep learning-based denoising method optofluidic device tuned for nanoparticle detection, we realize a analyzer that simultaneously achieves high...
Characterization and isolation of a large population cells are indispensable procedures in biological sciences. Flow cytometry is one the standards that offers method to characterize isolate at high throughput. When performing flow cytometry, molecularly stained with fluorescent labels adopt biomolecular specificity which essential for characterizing cells. However, molecular staining costly its chemical toxicity can cause side effects becomes critical issue when used downstream as medical...
We present a simple method to form free-standing lipid membranes on arrayed microchambers (>100). The formed are perpendicular an imaging plane with control of solute concentration each side the membranes. This platform let us quantitatively detect membrane transport non-charged fluorescent molecules, induced by proteins.
Local extracellular signaling is central for cellular interactions and organizations. We report a novel sensing technique to interrogate at the subcellular level. developed an in situ immunoassay based on giant optical enhancement of tunable nano-plasmonic-resonator array fabricated by nanoimprint lithography. Our nanoplasmonic device significantly increases signal-to-noise ratio enable first time submicrometer resolution quantitative mapping endogenous cytokine secretion. study shows...
We present a method for high-throughput optofluidic particle analysis that provides both the morphological and chemical profiles of individual particles in large heterogeneous population. This is based on an integration time-stretch optical microscope with submicrometer spatial resolution 780 nm three-color fluorescence analyzer top inertial-focusing microfluidic device. The integrated system can perform image- fluorescence-based screening high throughput 10,000 particles/s, exceeding...
3D light‐sheet microscopy is a powerful tool to obtain content‐rich information of cell populations. However, the limited frame rate high‐quantum‐efficiency cameras hinders its application in high‐throughput flow cytometry. Herein, 3D‐imaging cytometry technique based on that can screen thousands cells per second introduced. This method enables fast, parallel optofluidic scanning by performing 1D acoustofluidic focusing multiple under wide‐field with single objective lens. Multicolor...
Remotely manipulating a large number of microscopic objects is important to soft-condensed matter physics, biophysics, and nanotechnology. Optical tweezers optoelectronic have been widely used for this purpose but face critical challenges when applied nanoscale objects, including severe photoinduced damages, undesired ionic convections, or irreversible particle immobilization on surfaces. We report here the first demonstration lipid bilayer-integrated system simultaneous manipulation...
Abstract Early diagnosis and prompt initiation of appropriate treatment are critical for improving the prognosis acute leukemia. Acute leukemia is diagnosed by microscopic morphological examination bone marrow smears flow cytometric immunophenotyping cells stained with fluorophore‐conjugated antibodies. However, these diagnostic processes require trained professionals time resource‐intensive. Here, we present a novel approach using ghost cytometry, recently developed high‐content approach,...
Extracellular vesicles (EVs) are essential intercellular communication tools, but the regulatory mechanisms governing heterogeneous EV secretion still unclear due to lack of methods for precise analysis. Monitoring dynamics from individually isolated cells is crucial because in bulk analysis, activity can be perturbed by cell–cell interactions, and a cell population rarely performs magnitude- or duration-synchronized manner. Although various microfluidic techniques have been adopted evaluate...
Sanft dem Strom hinab: Mit einer Mikrofluidiktechnik werden in einem kontinuierlichen Fluidstrom monodisperse unilamellare Phospholipidvesikel aus einzigen Doppelschicht erzeugt (siehe Bild). Da die Vesikel robust sind und effizient eine Vielzahl an Molekülen hohen Konzentrationen einkapseln, eignen sie sich als Transportvehikel Modelle für Zellsysteme. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not...
We present a simple microfluidic method to generate high-density femotoliter-sized microreactor arrays within channels. In general, we designed main channel with many small chambers built into its walls. After sequentially infusing aqueous solution and organic solvent from single tube the device, droplets are confined in by flow. The generated reactors stable enough for carrying out ultrasensitive biochemical assays at molecule levels. As demonstration, this paper, optically observed...
Abstract Imaging flow cytometry shows significant potential for increasing our understanding of heterogeneous and complex life systems is useful biomedical applications. Ghost a recently proposed approach directly analyzing compressively measured signals cells, thereby relieving computational bottleneck real‐time data analysis in high‐throughput imaging cytometry. In previous work, we demonstrated that this image‐free could distinguish cells from two cell lines prepared with the same...
Three-dimensional (3D) fluorescence imaging is important to accurately capture and understand biological structures phenomena. However, because of its slow acquisition speed, it was difficult implement 3D for flow cytometry. Especially, modern cytometers operate at a velocity 1–10 m/s, no technique able cells such high velocity. Here, we present high-speed in which set optical cross sections cell captured within single frame camera by combining strobe light-sheet excitation optofluidic...