A5031086097- Heat shock proteins research
- Protein Structure and Dynamics
- Plant biochemistry and biosynthesis
- Cancer Genomics and Diagnostics
- Genomic variations and chromosomal abnormalities
- Vascular Tumors and Angiosarcomas
- Tuberous Sclerosis Complex Research
- Viral Infectious Diseases and Gene Expression in Insects
- Epigenetics and DNA Methylation
Brigham and Women's Hospital
2024
Harvard University
2024
Dartmouth College
2022
Icahn School of Medicine at Mount Sinai
2007-2008
Abstract Tuberous Sclerosis Complex (TSC) is caused by TSC1 or TSC2 mutations, leading to hyperactivation of mechanistic target rapamycin complex 1 (mTORC1) and lesions in multiple organs including lung (lymphangioleiomyomatosis) kidney (angiomyolipoma renal cell carcinoma). Previously, we found that TFEB constitutively active TSC. Here, generated two mouse models TSC which pathology the primary phenotype. Knockout rescues overall survival, indicating driver disease Importantly, increased...
Cdc37 is a molecular chaperone that functions with Hsp90 to promote protein kinase folding. Analysis of 65 Saccharomyces cerevisiae kinases (∼50% the kinome) in cdc37 mutant strain showed 51 had decreased abundance compared levels wild-type strain. Several lipid also accumulated reduced amounts Results from our pulse-labeling studies protects nascent chains rapid degradation shortly after synthesis. This phenotype was suppressed when cells were grown at temperatures, although this did not...
Ydj1 is a Saccharomyces cerevisiae Hsp40 molecular chaperone that functions with Hsp70 to promote polypeptide folding. We identified as being important for maintaining steady-state levels of protein kinases after screening several chaperones and cochaperones in gene deletion mutant strains. Pulse-chase analyses revealed portion Tpk2 kinase was degraded shortly synthesis ydj1Delta mutant, while the remainder capable maturing but reduced kinetics compared wild type. Cdc28 maturation also...
DNA methylation-based copy number variation (CNV) calling software offers the advantages of providing both genetic (copy-number) and epigenetic (methylation) state information from a single genomic library. This method is advantageous when looking at large-scale chromosomal rearrangements such as loss short arm chromosome 3 (3p) in renal cell carcinoma codeletion 1 long 19 (1p/19q) commonly seen histologically defined oligodendrogliomas. Herein, we present MethylMasteR: framework that...