A5036572160- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Lipid metabolism and biosynthesis
- interferon and immune responses
- Protein Kinase Regulation and GTPase Signaling
- RNA regulation and disease
- Diet, Metabolism, and Disease
- Ion Transport and Channel Regulation
- NF-κB Signaling Pathways
- Redox biology and oxidative stress
- Nerve injury and regeneration
- Caveolin-1 and cellular processes
- Alcohol Consumption and Health Effects
- Endoplasmic Reticulum Stress and Disease
- Ubiquitin and proteasome pathways
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Protein Degradation and Inhibitors
- Sulfur Compounds in Biology
- Cannabis and Cannabinoid Research
- Muscle Physiology and Disorders
- Lipid metabolism and disorders
- Wnt/β-catenin signaling in development and cancer
Novo Nordisk Foundation
2021-2024
University of Copenhagen
2021-2024
Lund University
2018-2021
Novo Nordisk Foundation Center for Basic Metabolic Research
2021
Abstract The mechanism of how patatin‐like phospholipase domain‐containing protein 3 (PNPLA3) variant M148 is associated with increased risk development hepatic steatosis still debated. Here, we propose a novel role PNPLA3 as key player during autophagosome formation in the process lipophagy. A human hepatocyte cell line, HepG2 cells, expressing recombinant I148 or 148M, was used to study lipophagy under energy deprived conditions, and lipid droplet morphology investigated using florescence...
The AMP-activated protein kinase (AMPK) gets activated in response to energetic stress such as contractions and plays a vital role regulating various metabolic processes insulin-independent glucose uptake skeletal muscle. main upstream that activates AMPK through phosphorylation of α-AMPK Thr172 muscle is LKB1, however some studies have suggested Ca2+/calmodulin-dependent 2 (CaMKK2) acts an alternative activate AMPK. We aimed establish whether CaMKK2 involved activation promotion following A...
Adipocytes play a central role in energy balance, and dysfunctional adipose tissue severely affects systemic homeostasis. The ATPase EH domain–containing 2 (EHD2) has previously been shown to regulate caveolae, plasma membrane-specific domains that are involved lipid uptake signal transduction. Here, we investigated the of EHD2 adipocyte function. We demonstrate protein expression is highly up-regulated at onset triglyceride accumulation during differentiation. Small interfering RNA–mediated...
Aquaporins play a crucial role in water homeostasis the human body, and recently physiological importance of aquaporins as glycerol channels have been demonstrated. The aquaglyceroporins (AQP3, AQP7, AQP9 AQP10) represent key channels, enabling flux across membranes cells. Adipocytes are major source during lipolysis, is released to be metabolized by other tissues through well-orchestrated process. Here we show that both AQP3 AQP7 bind lipid droplet protein perilipin 1 (PLIN1), suggesting...
AMP-activated protein kinase (AMPK) is a master regulator of cellular energy homeostasis and therapeutic target for metabolic diseases. Co/post-translational N-myristoylation glycine-2 (Gly2) the AMPK β subunit has been suggested to regulate distribution between cytosol membranes through "myristoyl switch" mechanism. However, relevance myristoylation signaling in cells vivo unclear. Here, we generated knockin mice with Gly2-to-alanine point mutation AMPKβ1 (β1-G2A). We demonstrate that...
Abstract Transcription Factor EB (TFEB) is a critical regulator of lysosomal biogenesis, autophagy and energy homeostasis through controlling expression genes belonging to the coordinated regulation network. AMP-activated protein kinase (AMPK) has been reported phosphorylate TFEB at three conserved C-terminal serine residues (S466, S467, S469) these phosphorylation events were essential for transcriptional activation TFEB. In sharp contrast this proposition, here we demonstrate that AMPK...