A5076643205- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
- Immunodeficiency and Autoimmune Disorders
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Protein Structure and Dynamics
- Muscle Physiology and Disorders
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Network Security and Intrusion Detection
- Cytomegalovirus and herpesvirus research
- Poxvirus research and outbreaks
- Digestive system and related health
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Anomaly Detection Techniques and Applications
- Immune responses and vaccinations
- RNA Research and Splicing
- Polyomavirus and related diseases
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Machine Learning in Bioinformatics
University of Pittsburgh
2015-2025
University of Minnesota
2023
Université Claude Bernard Lyon 1
2018
Pittsburg State University
2018
institut Camille-Jordan
2013-2014
Université Paris-Sud
2009-2013
Centre National de la Recherche Scientifique
2009-2013
Institut de Biochimie et Biophysique Moléculaire et Cellulaire
2010-2013
Université Paris Cité
2004-2007
Délégation Paris 7
2004-2007
Interventions against variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are urgently needed. Stable and potent nanobodies (Nbs) that target the receptor binding domain (RBD) SARS-CoV-2 spike promising therapeutics. However, it is unknown if Nbs broadly neutralize circulating variants. We found RBD highly resistant to concern (VOCs). High-resolution cryoelectron microscopy determination eight Nb-bound structures reveals multiple neutralizing epitopes clustered into...
ABSTRACT Bacteriophage T5 represents a large family of lytic Siphoviridae infecting Gram-negative bacteria. The low-resolution structure showed the T=13 geometry capsid and unusual trimeric organization tail tube, assembly pathway was established. Although major structural proteins have been identified in these studies, most genes encoding morphogenesis remained to be identified. Here, we combine proteomic analysis particles with bioinformatic study electron microscopic immunolocalization...
Large icosahedral viruses that infect bacteria represent an extreme of the coevolution capsids and genomes they accommodate. One subset these large is jumbophages, tailed phages with double-stranded DNA at least 200,000 bp. We explored mechanism leading to increased capsid genome sizes by characterizing structures several jumbophage packaged within them. Capsid determined for six jumbophages were consistent canonical phage HK97 fold, three had geometries novel triangulation numbers (T=25,...
Double-stranded DNA viruses utilise machinery, made of terminase proteins, to package viral into the capsid. For cos bacteriophage, a defined signal, recognised by small terminase, flanks each genome unit. Here we present first structural data for virus packaging motor, assembled from bacteriophage HK97 procapsids encompassing portal protein, and containing site. The cryo-EM structure is consistent with termination state adopted after cleavage, density within large assembly ending abruptly...
Green fluorescent proteins (GFP) are widely used in biology for tracking purposes. Although expression of GFP is considered to be innocuous the cells, deleterious effects have been reported. We recently demonstrated that eGFP muscle impairs its contractile properties (Agbulut, O., Coirault, C., Niederlander, N., Huet, A., Vicart, P., Hagege, Puceat, M., and Menasche, P. (2006) Nat. Meth. 3, 331). This prompted us identify molecular mechanisms linking dysfunction and, particularly, test...
Tailed bacteriophages and herpesviruses use a transient scaffold to assemble icosahedral capsids with hexameric capsomers on the faces pentameric at all but one vertex where 12-fold portal is thought nucleate assembly. How does orchestrate this step? We have determined structure of bacteriophage HK97 procapsid, domain major capsid protein. The forms rigid helix-turn-strand structures interior surfaces further stabilized around portal, forming trimeric coiled-coil towers, two per surrounding...
The large (90-nm) icosahedral capsid of bacteriophage T5 is composed 775 copies the major protein (mcp) together with portal, protease, and decoration proteins. Its assembly a regulated process that involves several intermediates, including thick-walled round precursor prohead expands as viral DNA packaged to yield thin-walled angular mature capsid. We investigated maturation by comparing cryoelectron microscopy (cryo-EM) structures prohead, empty expanded both without protein, virion at...
The herpes simplex virus (HSV) capsid is released into the cytoplasm after fusion of viral and host membranes, whereupon dynein-dependent trafficking along microtubules targets it to nuclear envelope. Binding pore complex (NPC) mediated by protein pUL25 capsid-tethered tegument pUL36. Temperature-sensitive mutants in both pUL36 dock at NPC but fail release DNA. uncoating reaction has been difficult study due rapid genome once interacts with pore. In this study, we describe isolation...
Abstract Bacteriophage capsids constitute icosahedral shells of exceptional stability that protect the viral genome. Many display on their surface decoration proteins whose structure and function remain largely unknown. The protein pb10 phage T5 binds at centre 120 hexamers formed by major capsid protein. Here we determined 3D investigated its capsid-binding properties using NMR, SAXS, cryoEM SPR. Pb10 consists an α-helical domain Ig-like exposed to solvent. It with a remarkably high...
The Siphoviridae coliphage T5 differs from other members of this family by the size its genome (121 kbp) and large icosahedral capsid (90 nm), which is organized with T=13 geometry. does not encode a separate scaffolding protein, but head pb8, contains 159-residue aminoterminal domain (Delta domain) that mature capsid. We have deciphered early events shell assembly starting purified pb8 Delta (pb8p). self pb8p regulated salt conditions leads to structures distinct morphologies. Expanded...
Abstract There is an urgent need to develop effective interventions resistant the evolving variants of SARS-CoV-2. Nanobodies (Nbs) are stable and cost-effective agents that can be delivered by novel aerosolization route treat SARS-CoV-2 infections efficiently. However, it remains unknown if they possess broadly neutralizing activities against prevalent circulating strains. We found potent Nbs highly convergent concern evade a large panel antibodies (Abs) significantly reduce convalescent or...
Abstract Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes use homomeric ring ATPase to processively translocate viral genomic into procapsids during assembly. Our current understanding packaging comes from three archetypal bacteriophage systems: cos, pac phi29. Detailed mechanistic exists for phi29, but not cos. Here, we reconstituted in...
Purpose/Aim: Elevated serum phosphate is one of the major factors contributing to vascular calcification. Studies suggested that extracellular vesicles released from smooth muscle cells significantly contribute initiation and progression this pathology. Recently, we have demonstrated elevated stimulates release osteogenic at mineralization process. Here, used MOVAS cell line as an in vitro model calcification examine whether respond high levels a similar way increase formation...
Herpes simplex virus 1 (HSV-1) is the causative agent of several pathologies ranging in severity from common cold sore to life-threatening encephalitic infection. A critical step during productive HSV-1 infection cleavage and packaging replicated, concatemeric viral DNA into preformed capsids. key knowledge gap how capsid engages replicated genome subsequent a unit-length HSV genome. Here, biochemical structural studies focused on unique portal vertex wild-type mutants provide insights...
Serum response factor (SRF) is a MADS transcription that binds to the CArG box sequence of serum element (SRE). Through its binding sequences, SRF activates several muscle‐specific genes as well respond mitogens. The thermodynamic parameters interaction core‐SRF (the 124–245 fragment factor) with specific oligonucleotides from c‐fos and desmin promoters, were determined by spectroscopy. rotational correlation time labeled bis‐ANS showed protein monomeric at low concentration (10 −7 m )....