A5016791338- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cell death mechanisms and regulation
- Genetics, Aging, and Longevity in Model Organisms
- Pluripotent Stem Cells Research
- Phagocytosis and Immune Regulation
University of Stuttgart
2021-2024
Extrinsic apoptosis relies on TNF-family receptor activation by immune cells or receptor-activating drugs. Here, we monitored cell cycle progression at a resolution of minutes to relate kinetics and cell-to-cell heterogeneities in death decisions phases. Interestingly, found that S phase delay TRAIL receptor-induced favour mitosis, thereby passing an apoptosis-primed state their offspring. This translates into two distinct fates, execution post mitosis survival from inefficient apoptosis....
Abstract The CRISPR/Cas9 technology has revolutionized genotype-to-phenotype assignments through large-scale loss-of-function (LOF) screens. However, limitations like editing inefficiencies and unperturbed genes cause significant noise in data collection. To address this, we introduce CRISPR Gene Transcriptome Engineering (CRISPRgate), which uses two specific sgRNAs to simultaneously repress cleave the target gene within same cell, increasing LOF efficiencies reproducibility. CRISPRgate...
Abstract Extrinsic apoptosis relies on TNF-family receptor activation by immune cells or receptor-activating biologics. Here, we monitored cell cycle progression at minutes resolution to relate kinetics and cell-to-cell heterogeneities in death decisions phases. Interestingly, found that S phase delay TRAIL receptor-induced favour for mitosis, thereby passing an apoptosis-primed state their offspring. This translates into two distinct fates, execution post mitosis survival from inefficient...