A5101859230- Aluminum toxicity and tolerance in plants and animals
- Heavy metals in environment
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- GABA and Rice Research
- Agriculture, Soil, Plant Science
- Aquaculture Nutrition and Growth
- Medicinal Plants and Neuroprotection
- Microbial bioremediation and biosurfactants
- Invertebrate Immune Response Mechanisms
- Crustacean biology and ecology
- Microbial Metabolic Engineering and Bioproduction
- Rice Cultivation and Yield Improvement
- Microbial Community Ecology and Physiology
China National Hybrid Rice R&D Central Hunan Hybrid Rice Reserch Center
2017-2024
State Key Laboratory of Hybrid Rice
2017-2024
Hunan University
2021-2022
Chinese Academy of Fishery Sciences
2021
State Key Joint Laboratory of Environment Simulation and Pollution Control
2009
Tsinghua University
2009
Rice grain with excessive cadmium (Cd) is a major source of dietary Cd intake and serious threat to health for people who consume rice as staple food. The development elite cultivars consistently low content challenging conventional breeding approaches, new strategies urgently need be developed. Here, we report the indica lines accumulation no transgenes by knocking out metal transporter gene OsNramp5 using CRISPR/Cas9 system. Hydroponic culture showed that concentrations in shoots roots...
OsNRAMP5 is a transporter responsible for cadmium (Cd) and manganese (Mn) uptake root-to-shoot translocation of Mn in rice plants. Knockout regarded as an effective approach to minimize Cd accumulation rice. It vital evaluate the effects knocking out on accumulation, well tolerance plants response varying environmental concentrations, uncover underlying mechanism, which until now, has remained largely unexplored. This study showed that knockout decreased uptake, but simultaneously...
Citric acid (CA) can regulate the balance of anions and cations in plants, improve their resistance to heavy metals. It is not clear if foliar application with CA has any effect on migration Cd Mn rice plant. In this work, a low-Cd-accumulating indica line (P7) high-Cd-accumulating (HZ) were used investigate influence transport as well amino metabolism grains. Content grains other organs increased increase content (0.1–2.4 mg kg−1) soil, while decreased CA. With grains, most acids HZ, P7, HZ...
Cadmium (Cd)-contaminated rice is a serious issue affecting food safety. Understanding the molecular regulatory mechanisms of Cd accumulation in grains crucial to minimizing concentrations grains. We identified member low-affinity cation transporter family, OsLCT2 rice. It was membrane protein. expressed all tissues elongation and maturation zones roots, with strongest expression pericycle stele cells adjacent xylem. When grown Cd-contaminated paddy soils, plants overexpressing significantly...
A rapid procedure was developed to screen for bacteria that are able grow on polycyclic aromatic hydrocarbons (PAHs).A drop of ethyl ether-dissolved PAH is spread a sterilized cellulose acetate/nitrate filter lying the top mineral salts agarose plate. After evaporation ether, serially diluted sample over and incubated. Subsequently, degrading can be counted isolated.This simple method screening bacterial isolates ability with PAHs.This technique and/or count PAH-degrading also used streak...
Penaeus japonicus is one of the most important farmed shrimp species in many countries. Sexual dimorphism observed P. japonicus, which females grow faster and larger than males; therefore, a unisexual female culture could improve efficiency productivity. However, genetic mechanisms underlying sex determination are unclear. In this study, we constructed high-density linkage map using genotyping-by-sequencing (GBS) technology full-sib family. The final was 3,481.98 cM length contained 29,757...
OsNRAMP5 is a pivotal gene involved in the uptake and transport of cadmium manganese rice plants. While knockout has significantly decreased accumulation grains, its impact on adaptability indica hybrid paternal parent still needs to be more adequately explored. This study conducted three-year field experiment with two lines (indica LYDG-1 DG-1) their wild-type at four distinct locations middle reaches Yangtze River. Their was evaluated by accumulation, yield traits, grain quality. The...