A5022691527- Forensic and Genetic Research
- Forensic Entomology and Diptera Studies
- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Insect behavior and control techniques
- Diptera species taxonomy and behavior
- Genetic diversity and population structure
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Yersinia bacterium, plague, ectoparasites research
- Microbial Community Ecology and Physiology
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Dermatological diseases and infestations
- Animal Disease Management and Epidemiology
- Protist diversity and phylogeny
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Insect-Plant Interactions and Control
- Carcinogens and Genotoxicity Assessment
- Species Distribution and Climate Change
- Genetic Associations and Epidemiology
- Data-Driven Disease Surveillance
- Legal and Policy Issues
- Chromatin Remodeling and Cancer
North Carolina State University
2018-2025
American Academy of Forensic Sciences
2023
Federal Bureau of Investigation
2014-2021
University of Wollongong
2009-2017
University of Florida
2013-2016
Queensland University of Technology
2014
University of Southern Queensland
1994
Taxonomic identification of biological materials can be achieved through DNA barcoding, where an unknown "barcode" sequence is compared to a reference database. In many disciplines, obtaining accurate taxonomic identifications imperative (e.g., evolutionary biology, food regulatory compliance, forensics). The Barcode Life DataSystems (BOLD) and GenBank are the main public repositories barcode sequences. this study, assessment accuracy reliability sequences in these databases was performed....
Rapid evolutionary radiations are expected to require large amounts of sequence data resolve. To resolve these types relationships many systematists believe that it will be necessary collect by next-generation sequencing (NGS) and use multispecies coalescent ("species tree") methods. Ultraconserved element (UCE) capture is becoming a popular method leverage the high throughput NGS address problems in vertebrate phylogenetics. Here we examine performance UCE for gallopheasants (true pheasants...
Abstract Carrion‐breeding insects, such as flesh flies (Diptera: Sarcophagidae), can be used evidence in forensic investigations. Despite their considerable potential, use has been limited because morphological species identification, at any life stage, is very challenging. This study investigated whether DNA could extracted and cytochrome oxidase subunit I ( COI ) barcode sequences obtained for molecular identification of each immature stage the forensically important Australian fly,...
The capacity to identify an unknown organism using the DNA sequence from a single gene has many applications. These include development of biodiversity inventories (Janzen et al. 2005), forensics (Meiklejohn 2011), biosecurity (Armstrong and Ball identification cryptic species (Smith 2006). popularity widespread use (Teletchea 2010) barcoding approach (Hebert 2003), despite broad misgivings (e.g., Smith 2005; Will Rubinoff 2006), attest this. However, one major shortcoming standard is that...
Porcine epidemic diarrhea virus (PEDV) is one of the most devastating pathogens global swine health and welfare. It well known that contaminated fomites vehicle movements play an important role in farm-to-farm PEDV spread, but efficacy cleaning disinfection (C&D) protocols on reduction dissemination risk via vehicles trailers remains unclear. This study used industry data to determine how frequently were with RNA before after C&D. Environmental samples collected at three eastern...
Environmental DNA from bulk materials can be analyzed to gain an understanding of the bacterial, fungal, plant, and/or arthropod communities present. metabarcoding is widely used characterize these biological communities, by amplifying “barcode” regions and sequencing amplicons via next-generation sequencing. The Earth Microbiome Project (EMP) adopted use indexed primers, PCR primers containing Illumina® adapter sequences a unique 12-nucleotide Golay barcode simplify identification bacterial...
The most striking feature of peafowl (Pavo) is the males' elaborate train, which exhibits ocelli (ornamental eyespots) that are under sexual selection. Two additional genera within Phasianidae (Polyplectron and Argusianus) exhibit ocelli, but appearance location these ornamental eyespots substantial variation among genera, raising question whether homologous. Within Polyplectron, ancestral, suggesting may have evolved even earlier, prior to divergence genera. However, it remains unclear...
Ancestry inference for a person using panel of SNPs depends on the variation frequencies those around world and amount reference data available calculation/comparison. The Kidd Lab 55 AISNPs has been incorporated in commercial kits by both Life Technologies Illumina massively parallel sequencing. Therefore, larger set populations will be useful researchers kits. We have added population allele 52 samples to 73 previously entered so that there are now publicly ALFRED FROG-kb total 125 samples.
Molecular-based taxonomy, specifically DNA barcoding, has streamlined organism identification. For land plants, the recommended 2-locus barcode of rbcL and matK is not suitable for all groups, thus second subunit nuclear internal transcribed spacer (ITS2) received attention as a possible alternative. To date, evaluations ITS2 have mostly been limited in scope to specific plant orders/families single source material. Prior using routinely characterize plants present environmental samples...
Forensically relevant single nucleotide polymorphisms (SNPs) can provide valuable supplemental information to short tandem repeats (STRs) for investigative leads, and genotyping now be streamlined using massively parallel sequencing (MPS). Dust is an attractive evidence source, as it accumulates on undisturbed surfaces, often overlooked by perpetrators, contains sufficient human DNA analysis. To assess whether SNPs genotyped from indoor dust MPS could used detect known household occupants,...
Carrion-breeding Sarcophagidae (Diptera) can be used to estimate the post-mortem interval in forensic cases. Difficulties with accurate morphological identifications at any life stage and a lack of documented thermobiological profiles have limited their current usefulness. The molecular-based approach DNA barcoding, which utilises 648-bp fragment mitochondrial cytochrome oxidase subunitI gene, was evaluated pilot study for discrimination between 16 Australian sarcophagids. comprehensively...
Wildlife forensic analyses are frequently concerned with taxonomic identification, and very often employ amplification Sanger sequencing of informative regions the genome to achieve this. The materials submitted wildlife laboratories for identification span a wide scope, from plant animal parts in trade assemblages incidental biota at crime scenes. As these take place within context legal proceedings, community is subject unique requirements considerations. These considerations quite...
The Australian Sarcophagidae (Diptera) currently comprise 84 species, classified into ten genera from the subfamilies Miltogramminae and Sarcophaginae. A key is provided to sarcophagids, allowing for separation sub-families genera, along with identification of all species Sarcophaga (sensu lato). comprehensive database illustrations photographs male terminalia, as well updated biological information, given each s.l.
Parchment, the skins of animals prepared for use as writing surfaces, offers a valuable source genetic information. Many have clearly defined provenance, allowing findings to be evaluated in temporal and spatial context. While these documents can yield evidence animal sources, DNA contained within aged is often damaged fragmented. Previously, studies targeting parchment used destructive sampling techniques so development validation non-destructive methods would expand opportunities...
Abstract Oculocutaneous Albinism type 2 ( OCA2 ) is a gene of great interest because genetic variation affecting normal pigmentation in humans. The diverse geographic patterns for variant frequencies at have been evident but not systematically investigated, especially outside Europe. Here we examine population and near the from worldwide perspective. very different found across world regions suggest strong selection effects may work over time. For example, analyses involving variants that...