A5050529203- Mechanical Behavior of Composites
- Advanced biosensing and bioanalysis techniques
- Cellular and Composite Structures
- Plant biochemistry and biosynthesis
- Toxic Organic Pollutants Impact
- Advanced Manufacturing and Logistics Optimization
- Supply Chain and Inventory Management
- Natural Fiber Reinforced Composites
- Plant Gene Expression Analysis
- Scheduling and Optimization Algorithms
- Manufacturing Process and Optimization
- Microbial Natural Products and Biosynthesis
- Pharmaceutical and Antibiotic Environmental Impacts
- Environmental remediation with nanomaterials
- Advanced Biosensing Techniques and Applications
- Graphene and Nanomaterials Applications
- Structural Health Monitoring Techniques
- Fuel Cells and Related Materials
- Structural Behavior of Reinforced Concrete
- Electrocatalysts for Energy Conversion
- Seismic Performance and Analysis
- Textile materials and evaluations
- Composite Structure Analysis and Optimization
- Additive Manufacturing and 3D Printing Technologies
- Transportation Safety and Impact Analysis
Hunan University
2016-2025
Guizhou Provincial People's Hospital
2025
Nanjing Institute of Environmental Sciences
2023
Ministry of Ecology and Environment
2021-2023
Hunan Academy of Agricultural Sciences
2021-2023
Fudan University
2019-2023
Hunan Agricultural Products (China)
2022-2023
China University of Petroleum, Beijing
2020
State Key Laboratory of Chemobiosensing and Chemometrics
2015-2017
Chinese Academy of Sciences
2009
Protein engineering by resurfacing is an efficient approach to provide new molecular toolkits for biotechnology and bioanalytical chemistry. H39GFP a variant of green fluorescent protein (GFP) containing 39 histidine residues in the primary sequence that was developed resurfacing. Herein, taking as signal reporter, label-free fluorometric sensor Cu(2+) sensing based on unique multivalent metal ion-binding property fluorescence quenching effect electron transfer. The high affinity with (Kd,...
Abstract Metallizing active sites to control the structural and kinetic dissociation of water at catalyst–electrolyte interface, along with elucidating its mechanism under operating conditions, is a pivotal innovation for hydrogen evolution reaction (HER). Here, design singly dispersed Pt–Co in fully metallic state on nanoporous Co 2 P, tailored HER, introduced. An anion‐exchange‐membrane electrolyzer equipped this catalyst can achieve industrial current densities 1.0 2.0 A cm −2 1.71 1.85...
Considering vehicle crash safety, gas emission and the improvement in energy efficiency, carbon fiber reinforced plastic (CFRP) composite materials have been increasingly used automotive applications. Bumper beam, as a main structural component of automobile bumper subsystem, is expected to protect occupants its nearby components. It an effective way develop beam using CFRP meet higher requirements safety lightweight. In this study, stiffness degraded model proposed predict low-velocity...
Enzyme promiscuity is evolutionarily advantageous to plants for gaining new enzyme functions when adapting environmental challenges. However, this can negatively affect the expression of genes encoding plant enzymes in microorganisms. Here, we show that refining flavonoid 3'-hydroxylase (F3'H) and 4'-O-methyltransferase (F4'OMT) improves (2S)-hesperetin production Escherichia coli. First, employed inverse molecular docking screen a highly substrate-specific ThF3'H from Tricyrtis hirta, which...
A single-atom Pt and phosphorus co-doping strategy is applied to a nanoporous MoS 2 catalyst, where P dopants could modulate the electronic structure around atoms, leading significantly improved activity for PEM electrolyzer applications.
Protein phosphorylation catalyzed by protein kinases plays a critical role in many intracellular processes, and detecting kinase activity is important biochemical research drug discovery. Herein, we developed novel fluorescent biosensor to detect based on phosphorylation-mediated assembly of semisynthetic green (GFP). A chimaera S-peptide composed the 10th β-strand GFP (s10) substrate peptide was synthesized. Kinase-catalyzed can protect its s10 part against cleavage carboxypeptidase Y...
Nowadays, large-scale screening for enzyme discovery, engineering, and drug discovery processes require simple, fast, sensitive activity assay platforms with high integration potential high-throughput detection. Herein, a novel automatic integrated micro-enzyme (AIμEA) platform was proposed based on unique microreaction system fabricated by engineered green fluorescence protein (GFP)-functionalized monolithic capillary column, thrombin as an example. The recombinant GFP probe rationally to...
The interaction between supercharged green fluorescent protein (ScGFP) and graphene oxide (GO) as well the resulting quenching effect of GO on ScGFP were investigated.