A5055507176- Graphene and Nanomaterials Applications
- Bone Tissue Engineering Materials
- 3D Printing in Biomedical Research
- Plant Stress Responses and Tolerance
- Cell Image Analysis Techniques
- Plant Micronutrient Interactions and Effects
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Image Processing Techniques and Applications
- Aluminum toxicity and tolerance in plants and animals
- Genomics and Chromatin Dynamics
- Biosensors and Analytical Detection
- Metabolism, Diabetes, and Cancer
- Collagen: Extraction and Characterization
- Cell death mechanisms and regulation
- Single-cell and spatial transcriptomics
- AI in cancer detection
- Microencapsulation and Drying Processes
- Alkaloids: synthesis and pharmacology
- Molecular Junctions and Nanostructures
- Kruppel-like factors research
- Phytochemical compounds biological activities
- Fullerene Chemistry and Applications
- Animal Genetics and Reproduction
University of Wisconsin–Milwaukee
2022-2024
University of Nevada, Reno
2015-2022
Lawrence Berkeley National Laboratory
2015-2020
University of South Carolina
2013-2016
Sichuan University
2009-2010
Abstract A 3D spongy collagen cryogel was prepared using DAS as the cross‐linker. FTIR and CD studies demonstrate that crosslinking is achieved through reaction of aldehyde groups with free amino in without affecting triple helix collagen. SEM demonstrates has a heteroporous structure interconnecting pores. DSC measurements reveal cryogels have improved thermal stability comparison pure Moreover, ESR shows water uptake decreases content. Evaporation tests indicate can hold moisture for long...
Cancer has arisen to be of the most prominent health care issues across world in recent years. Doctors have used physiological intervention as well chemical and radioactive therapeutics treat cancer thus far. As an alternative current methods, gene delivery systems with high efficiency, specificity, safety that can reduce side effects such necrosis tissue are under development. Although viral vectors highly efficient, concerns from fact sourced lethal diseases. With this mind, rod shaped...
Current methods of treating critical size bone defects include autografts and allografts, however, both present major limitations including donor-site morbidity, risk disease transmission, immune rejection. Tissue engineering provides a promising alternative to circumvent these shortcomings through the use autologous cells, three-dimensional scaffolds, growth factors. We investigated development scaffold with native extracellular matrix (ECM) components for directing osteogenic...
The success of bone tissue engineering strategies critically depends on the rapid formation a mature vascular network in scaffolds after implantation.Conventional methods to accelerate infiltration host vasculature into need consider role response regulation ingrowth and extent vascularization.The long term goal this study was harness potential inflammatory enhance angiogenesis three dimensional (3D) scaffolds.Towards goal, we explored use resveratrol, natural compound commonly used...
BioSig3D is a computational platform for high-content screening of three-dimensional (3D) cell culture models that are imaged in full 3D volume. It provides an end-to-end solution designing high content assays, based on colony organization derived from segmentation nuclei each colony. also enables visualization raw and processed volumetric data quality control, integrates advanced bioinformatics analysis. The system consists multiple annotation modules coupled together with strong use...
Abstract The effects of the stiffness microenvironment on molecular response 3D colony organization, at maximum level mammographic density (MD), are investigated. Phenotypic profiling reveals that formation is heterogeneous and increased microenvironment, within range MD, correlates with frequency aberrant formation. Further integrative analysis genome-wide transcriptome phenotypic hypothesizes overexpression ERBB2 in premalignant MCF10A cell lines a value corresponds to collagen component...
Reprogramming the tumor stroma is an emerging approach to circumventing challenges of conventional cancer therapies. This strategy, however, hampered by lack a specific molecular target. We previously reported that stromal fibroblasts (FBs) with high expression CD36 could be utilized for this purpose. These studies are now expanded identify secreted factors responsible suppression. Methodologies included 3D colonies, fluorescent microscopy coupled quantitative techniques, proteomics...
Cancer has arisen to be of the most prominent health care issues across world in recent years.Doctors have used physiological intervention as well chemical and radioactive therapeutics treat cancer thus far.As an alternative current methods, gene delivery systems with high efficiency, specificity, safety that can reduce side effects such necrosis tissue are under development.Although viral vectors highly efficient, concerns from fact sourced lethal diseases.With this mind, rod shaped...
Abstract Motivation Our previous study has shown that ERBB2 is overexpressed in the organoid model of MCF10A when stiffness microenvironment increased to high mammographic density (MD). We now aim identify key transcription factors (TFs) and functional enhancers regulate processes associated with models premalignant human mammary cell lines. Results 3D colony organizations cis-regulatory networks two epithelial lines (184A1 MCF10A) are investigated as a function within range MD. The colonies...
Abstract To date, in situ visualization of microbial density has remained an open problem. Here, functionalized buckyballs ( e.g. , C60-pyrrolidine tris acid) are shown to be a versatile platform that allows internalization within microorganism without either adhering the cell wall and membrane or binding matrix substrate such as soil. These molecular probes validated via multi-scale imaging, show association with microorganisms fluorescence microscopy, positive cellular uptake electron...
Tumor and stroma coevolve to facilitate tumor growth. Hence, effective therapeutics would not only induce growth suppression of cells but also revert pro-tumor into anti-tumoral type. Previously, we showed that coculturing triple-negative or luminal A breast cancer with CD36+ fibroblasts (FBs) in a three-dimensional extracellular matrix induced their phenotypic reversion, respectively. Then, identified SLIT3, FBLN-1, PENK as active protein ligands secreted from FBs MDA-MB-231 determined...
A new, effective and economical method to extract ardicrenin from Ardisia crenata Sims collected in the Wolong natural reserve, Sichuan, China, is established. powder counter-current extracted with 80% methanol reflux, decompressively enriched centrifuged defat. Supernatant applied macroporous resin column (AB-8) methanol, isolated by silica gel chromatography dichlormethane-acetoacetate-methanol (4:1.5:1) washing, recrystallized methanol. The final product which proved be analytic procedure...
Stem cells offer a promising tool in tissue engineering strategies, as their differentiated derivatives can be used to reconstruct most biological tissues. These approaches rely on controlling the biophysical cues that tune ultimate fate of cells. In this context, significant effort has gone parse out role conflicting matrix-elicited signals (e.g., topography and elasticity) regulation macroscopic characteristics shape polarity). A critical hurdle, however, lies our inability recapitulate...
This research introduces an advanced approach to automate the segmentation and quantification of nuclei in fluorescent images through deep learning techniques. Overcoming inherent challenges such as variations pixel intensities, noisy boundaries, overlapping edges, our devised pipeline integrates U-Net architecture with state-of-the-art CNN models, EfficientNet. fusion maintains efficiency while harnessing superior capabilities Crucially, we exclusively utilize high-quality confocal...