A5068161089- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Cystic Fibrosis Research Advances
- Chromosomal and Genetic Variations
- Bacteriophages and microbial interactions
- Marine and coastal plant biology
- Developmental Biology and Gene Regulation
- Fetal and Pediatric Neurological Disorders
- Micro and Nano Robotics
Université Paris-Saclay
2009-2021
Institut de Biologie Intégrative de la Cellule
2015-2020
Centre National de la Recherche Scientifique
2010-2019
CEA Paris-Saclay
2015-2019
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015-2019
Centre de Génétique Moléculaire
2012-2017
Stabilité génétique et oncogenèse
2015
Biogen (Switzerland)
2015
Université Paris-Sud
2010-2014
Université Paris Cité
2009
Ciliary shedding occurs from unicellular organisms to metazoans. Although required during the cell cycle and neurogenesis, process remains poorly understood. In all cellular models, this phenomenon distal transition zone (TZ), suggesting conserved molecular mechanisms. The TZ module proteins (Meckel Gruber syndrome [MKS]/Nephronophtysis [NPHP]/Centrosomal protein of 290 kDa [CEP290]/Retinitis pigmentosa GTPase regulator-Interacting Protein 1-Like [RPGRIP1L]) are known cooperate establish...
Within the FOP family of centrosomal proteins, conserved FOR20 protein has been implicated in control primary cilium assembly human cells. To ascertain its role ciliogenesis, we have investigated function ortholog, PtFOR20p, a multiciliated unicellular organism, Paramecium. By combined functional and cytological analysis, found that PtFOR20p specifically localizes at basal bodies is required to build transition zone, prerequisite their maturation docking cell surface, hence ciliogenesis. We...
First discovered in unicellular eukaryotes, centrins play crucial roles basal body duplication and anchoring mechanisms. While the evolutionary status of founding members family, Centrin2/Vfl2 Centrin3/cdc31 has long been investigated, origin other family received less attention. Using a phylogeny ciliate centrins, we identify two centrin families, ciliary present contractile filaments (ICL centrins). In this paper, carry on functional analysis still not well-known ICL1e subfamily identified...
ObjectivesThe assembly of cilia can be followed step by in the unicellular Paramecium, thanks to its predictable spatiotemporal pattern basal body (BB) duplication and ciliary growth.In order dissect process transition zone (TZ) assembly, we compared behaviour several proteins. MethodsCombination EM, immunocytochemistry, protein tagging RNAi knockdowns.
Objective The Transition Zone (TZ) is defined as the most proximal region of cilium overlapping with distal basal body. This zone has been shown to play a crucial role in cilia biology since it considered site sorting proteins that transit cilia. Protein complexes housed at this are found mutated MKS/NPHP ciliopathies. Although its organization varies from organism organism, TZ molecular composition and function highly conserved. In Paramecium, well structured three distinct plates terminal,...
Abstract Primary ciliary dyskinesia (PCD) is a genetically and phenotypically heterogeneous disorder characterized by destructive respiratory disease laterality abnormalities due to randomised left-right body asymmetry. PCD mostly caused mutations affecting components of the core axoneme structure motile cilia that are essential for movement. In addition, there growing group genes encode proteins assembly dynein motors active transport process delivers them from their cytoplasmic site into...
In the hypotrich ciliate Euplotes, many individual basal bodies are grouped together in tightly packed clusters, forming ventral polykinetids. These groups of (which produce compound ciliary organelles such as cirri and oral membranelles) cross-linked into ordered arrays by scaffold structures known "basal-body cages." The major protein comprising Euplotes cages has been previously identified termed "cagein." Screening a E. aediculatus cDNA expression library with anti-cagein antisera DNA...
ObjectivesCiliogenesis is conditioned by a correct positioning/ anchoring of the basal body at cell surface.In Paramecium we have shown that three conserved proteins FOR20, centrin 2 (CEN2) and 3 (CEN3) participates in this process, with FOR20 CEN2 being also involved transition zone assembly.We established chronology assembly: required for recruitment, latter necessary to recruit CEN3.Our goal now integrate others molecules cascade.
The transition zone (TZ) of locomotory cilia participates in docking young basal bodies to the surface, stabilizes body-membrane connection, forms a diffusion barrier and modulates intraciliary transport. In vertebrate ciliated cells, nucleoporins septins were suggested be present TZ primary cilia. Here we data suggesting presence nucleoporins, at both nuclear pores ciliary TZ, Tetrahymena Paramecium. Using monoclonal 414, specific FG-containing anti-tubulin antibodies, detected labeling...
ABSTRACT Ciliogenesis is a general process in eukaryotic cells and its different steps begin to be well characterised. However, the molecular mechanisms leading decilation or ciliary shedding are still poorly understood. This process, observed from unicellular organisms such as Chlamydomonas Paramecium multiciliated trachea fallopian tube of vertebrates, seems since recent observations demonstrates requirement during cell cycle neurogenesis. Interestingly, all cellular models, occurs distal...