A5004023398- Flame retardant materials and properties
- biodegradable polymer synthesis and properties
- Synthesis and properties of polymers
- RNA Interference and Gene Delivery
- Protein purification and stability
- Antimicrobial agents and applications
- Polymer Surface Interaction Studies
- Hepatitis C virus research
- Monoclonal and Polyclonal Antibodies Research
- Advancements in Transdermal Drug Delivery
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Advanced Drug Delivery Systems
- Toxin Mechanisms and Immunotoxins
- Surfactants and Colloidal Systems
- Nanoparticle-Based Drug Delivery
- Nanocomposite Films for Food Packaging
- Advanced Polymer Synthesis and Characterization
- Silicone and Siloxane Chemistry
- Surface Modification and Superhydrophobicity
- HIV Research and Treatment
- Mast cells and histamine
- Dendrimers and Hyperbranched Polymers
- Inhalation and Respiratory Drug Delivery
- Click Chemistry and Applications
Institute for Bioscience and Biotechnology Research
2016-2025
Advanced Bioscience Laboratories (United States)
2016-2025
University of Maryland, Baltimore
2019-2024
University of Maryland, College Park
2015-2024
National Institute of Standards and Technology
2019-2024
Apogee Technology (United States)
2009-2014
Parallel Consulting (United States)
2004-2006
Gidropribor
2001
Oncotherapeutics (United States)
2000
Massachusetts Institute of Technology
1993-1994
Intradermal immunization using microfabricated needles represents a potentially powerful technology, which can enhance immune responses and provide antigen sparing. Solid vaccine formulations, be coated onto microneedle patches suitable for simple administration, also offer improved shelf-life. However the approach is not fully compatible with many adjuvants including alum, most common adjuvant used in market globally. Here, we introduce polyphosphazene immuno as biologically potent...
Abstract HIV-1 broadly neutralizing antibodies (bNAbs) are being explored as passively administered therapeutic and preventative agents. However, the extensively diversified envelope glycoproteins (Env) rapidly acquire mutations to evade individual bNAbs in monotherapy regimens. The use of a “single” agent simultaneously target distinct Env epitopes is desirable overcome viral diversity. Here, we report tandem single-chain variable fragment (ScFv) domains two bNAbs, specific for CD4-binding...
Studies on the biological performance of nanomedicines have been increasingly focused paradigm shifting role protein corona, which is imminently formed once formulation placed in a complex physiological environment. This phenomenon predominantly studied context adsorption science, while such interactions for water-soluble systems remain virtually unexplored. In particular, importance plasma binding yet to be understood pharmaceuticals designed basis supramolecular architectures, generally...
Polyphosphazene polyacids show potential as immunostimulating compounds and materials for microencapsulation. Their synthesis requires multistep chemical transition from a hydrolytically unstable macromolecular precursor, poly(dichlorophosphazene), to water-soluble polyelectrolyte. Insufficient synthetic control in these reactions can lead molecular weight variations formation of macromolecules with "structural defects" resulting significant polymer performance. Simple reproducible "one...
Polyphosphazene polyelectrolytes are potent immunostimulants. Their in vivo performance has been demonstrated for various antigens a number of animal models. To improve understanding the mechanism action, we performed comparative study model system: bovine serum albumin, BSA−poly[di(carboxylatophenoxy)phosphazene], PCPP, vitro and vivo. Multi-angle laser light scattering (MALLS) size-exclusion HPLC methods were used to investigate polyphosphazene−protein formulations an attempt establish...
Hepatitis C virus (HCV) is a major public health concern, and the development of an effective HCV vaccine plays important role in effort to prevent new infections. Supramolecular co-assembly co-presentation envelope E1E2 heterodimer complex core protein presents attractive design strategy for achieving humoral cellular immunity. With this objective, two antigens were non-covalently assembled with immunostimulant (TLR 7/8 agonist) into virus-mimicking polymer nanocomplexes (VMPNs) using...
An effective vaccine against hepatitis C virus (HCV) must elicit the production of broadly neutralizing antibodies (bnAbs) reproducibly E1E2 glycoprotein complex. Little is known about how glycan content affects this process. Ideally, glycans would maximize epitope exposure without compromising antigen stability or exposing new epitopes. However, typical recombinant vaccines contain considerable heterogeneity in content, which can affect antibody response and neutralization potency. Here we...
A new water-soluble polyphosphazene polyelectrolyte containing carboxylate functionalities, poly[di(sodium carboxylatoethylphenoxy)phosphazene] (PCEP) was synthesized via reaction of macromolecular substitution. The polymer characterized using (1)H, (31)P NMR, and gel permeation chromatography with multiangle laser light scattering detection. PCEP shown to undergo hydrolytic degradation in aqueous solutions, as indicated by the decrease molecular weight release side groups. series...
Water-soluble polyphosphazenes emerge as an important class of biologically active macromolecular compounds dictating the need for development their well-defined and controlled synthesis. The synthetic pathway leading to involves preparation chemical transformation inorganic precursorpoly(dichlorophosphazene), PDCP. Synthesis, stabilization, characterization this hydrolytically sensitive, reactive intermediate are focus present study. Ring-opening polymerization reaction PDCP has been...
Degradation of a water-soluble polyphosphazene, poly[di(carboxylatophenoxy)phosphazene], disodium salt (PCPP) has been studied in aqueous solutions at elevated temperature. This synthetic polyelectrolyte is interest as vaccine adjuvant and its degradability constitutes an important component safety formulation stability profiles. The degradation process manifested by gradual reduction the molecular weight polymer cleavage side groups, which consistent with previously reported data on...
Two macromolecular immunoadjuvants, poly[di(carboxylatophenoxy)phosphazene], PCPP, and poly[di(carboxylatoethylphenoxy)phosphazene], PCEP, have been investigated for their molecular interactions with model biopharmaceutically important proteins in solutions, as well TLR stimulatory effects pH-dependent membrane disruptive activity cellular assays. Solution between polyphosphazenes proteins, including antigens soluble immune receptor studied using Asymmetric Flow Field Fractionation (AF4)...
A series of biodegradable drug delivery polymers with intrinsic multifunctionality have been designed and synthesized utilizing a polyphosphazene macromolecular engineering approach. Novel water-soluble polymers, which contain carboxylic acid pyrrolidone moieties attached to an inorganic phosphorus-nitrogen backbone, were characterized by suite physicochemical methods confirm their structure, composition, molecular sizes. All polyphosphazenes displayed composition-dependent hydrolytic...
A novel series of peptide based hybrid polymers designed to undergo enzymatic degradation is presented, via macrosubstitution a polyphosphazene backbone with the tetrapeptide Gly-Phe-Leu-Gly. Further co-substitution hydrophilic polyalkylene oxide Jeffamine M-1000 leads water soluble and biodegradable polymers. Detailed studies, 31P NMR spectroscopy, dynamic light scattering field flow fractionation show degrade combination enzymatic, as well hydrolytic pathways. The sequence was chosen due...
Abstract Polyorganophosphazenes are water-soluble macromolecules with immunoadjuvant activity that self-assemble proteins to enable biological functionality. Direct imaging by cryogenic electron microscopy uncovers the coil structure of those highly charged macromolecules. Here, we successfully visualize individual polymer chains within vitrified state in absence additives for contrast enhancement which is attributed high mass inorganic backbone. Upon assembly proteins, multiple protein...