A5009758229- Advanced Proteomics Techniques and Applications
- Single-cell and spatial transcriptomics
- Mass Spectrometry Techniques and Applications
- Catalytic Alkyne Reactions
- Synthetic Organic Chemistry Methods
- Cyclopropane Reaction Mechanisms
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Marine Sponges and Natural Products
- Innovative Microfluidic and Catalytic Techniques Innovation
- Phytochemistry and Bioactivity Studies
- Scientific Computing and Data Management
- Metabolomics and Mass Spectrometry Studies
- Chemical Synthesis and Analysis
- Sulfur-Based Synthesis Techniques
- Traditional and Medicinal Uses of Annonaceae
- Oxidative Organic Chemistry Reactions
- Chemical synthesis and alkaloids
- Click Chemistry and Applications
- Alkaloids: synthesis and pharmacology
- Biosensors and Analytical Detection
- Pickering emulsions and particle stabilization
- Gene Regulatory Network Analysis
- Phytochemistry and Bioactive Compounds
- Catalysis for Biomass Conversion
Universidad del Noreste
2022-2025
Northeastern University
2021-2024
Scienion (Germany)
2022
Lehigh University
2017
Massachusetts Institute of Technology
2011-2013
University West
2007-2010
Western University
2006-2009
Many biological processes, such as cell division cycle and drug resistance, are reflected in protein covariation across single cells. This can be quantified interpreted by single-cell mass spectrometry with sufficiently high throughput accuracy.
Major aims of single-cell proteomics include increasing the consistency, sensitivity and depth protein quantification, especially for proteins modifications biological interest. Here, to simultaneously advance all these aims, we developed prioritized Single-Cell ProtEomics (pSCoPE). pSCoPE consistently analyzes thousands peptides across single cells (thus data completeness) while maximizing instrument time spent analyzing identifiable peptides, thus proteome depth. These strategies increased...
The reaction of benzyl-protected propargyl amines and 1,1-cyclopropane diesters in the presence catalytic Zn(NTf2)2 allows access to highly functionalized piperidines excellent yields. process proceeds via a tandem cyclopropane ring-opening/Conia−ene cyclization.
Safe and secure: An efficient methodology which provides access to homochiral 2,5-cis pyrrolidines in excellent yields starting from chiral alkoxyamine cyclopropanes was used the total synthesis of (−)-allosecurinine (see scheme). The proceeds with enantiomeric purity 15 steps an overall yield 5 %. OTf: trifluoromethanesulfonate, Boc: tert-butoxycarbonyl, PG: protecting group. Supporting information for this article is available on WWW under...
To study mesenchymal-epithelial interactions associated with the normal and pathological human prostate, we have developed a model of well differentiated prostate epithelial fibroblastic cells. Normal prostatic cells, either or origins were successfully transfected SV40 strains extended lifespan selected until crisis was reached, within 20 30 passages for respectively. Only few clones emerged from crisis: PNT1A (Cussenot et al: J Urol 143: 881-886, 1991), PNT1B PNT2 cell lines. Successful...
We report a method for the oxidation of range alcohols and aldehydes utilizing simple flow system in EtOAc with stream 12.5% NaOCl catalytic Bu4NBr. Secondary are oxidized to ketones, directly methyl esters presence methanol, benzylic either benzaldehydes or esters, depending on conditions used. The reaction mild generally provide complete conversion 5–30 min.
Go with the flow: A general approach for amide bond formation by way of a continuous-flow photochemical rearrangement nitrones was described (see scheme). Simple aryl-alkyl bonds as well complex peptide were constructed efficiently residence time less than 20 minutes. tetrapeptide synthesized in this and method could be applied to fragment coupling. As service our authors readers, journal provides supporting information supplied authors. Such materials are peer reviewed may re-organized...
Many biological processes, such as the cell division cycle, are reflected in protein covariation across single cells. This can be quantified and interpreted by single-cell mass-spectrometry (MS) with sufficiently high throughput accuracy. Towards this goal, we developed nPOP, a method that uses piezo acoustic dispensing to isolate individual cells 300 picoliter volumes performs all subsequent sample preparation steps small droplets on fluorocarbon-coated slide. design enabled simultaneous of...
Cellular protein concentrations are maintained through a balance of synthesis and clearance. Clearance occurs both degradation growth-dependent dilution. At slow growth, clearance is dominated by degradation, which leads to the accumulation long lived proteins. fast however, it dilution, preventing this accumulation. Thus, concentration proteins will be reduced unless cells compensate preferentially increasing rates. To determine dominant regulatory mechanisms, we quantified degree...
Lewis acid catalyzed ring opening of 1,1-cyclopropanediesters by the hydroxyl group a propargyl alcohol sets up subsequent Conia-ene cyclization to afford substituted tetrahydropyrans in one-pot, high-yielding procedure.
The physiological response of a cell to stimulation depends on its proteome configuration. Therefore, the abundance variation regulatory proteins across unstimulated single cells can be associatively linked with their stimulation. Here we developed an approach that leverages this association individual and nuclei systematically identify potential regulators biological processes, followed by targeted validation. Specifically, applied nucleocytoplasmic protein transport in macrophages...
Major aims of single-cell proteomics include increasing the consistency, sensitivity, and depth protein quantification, especially for proteins modifications biological interest. To simultaneously advance all these aims, we developed prioritized Single Cell ProtEomics (pSCoPE). pSCoPE consistently analyzes thousands peptides across single cells (thus data completeness) while analyzing identifiable at full duty-cycle, thus proteome depth. These strategies increased completeness, coverage over...
Abstract Limiting artifacts during sample preparation can significantly increase data quality in single-cell proteomics experiments. Towards this goal, we characterize the impact of protein leakage by analyzing thousands primary single cells that were prepared either fresh immediately after dissociation or cryopreserved and at a later date. We directly identify permeabilized use to define signature for leakage. build classifier identifying damaged performs accurately across cell types species.
Amino acid substitutions may substantially alter protein stability and function, but the contribution of arising from alternate translation (deviations genetic code) is unknown. To explore it, we analyzed deep proteomic transcriptomic data over 1,000 human samples, including 6 cancer types 26 healthy tissues. This global analysis identified 60,024 high confidence corresponding to 8,801 unique sites in proteins derived 1,990 genes. Some are shared across while others exhibit strong...
Abstract The first preparation of the antimalarial natural product decursivine is described. A Diels–Alder/Plieninger indolization protocol allows convenient indole 15 which, in turn a suitable substrate for boron–enolate aldol reaction with piperonal ( 16 ). resulting adduct 14 transformed efficiently to product. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2007)
Current mass-spectrometry methods enable high-throughput proteomics of large sample amounts, but low amounts remains limited in depth and throughput. To increase the throughput sensitive proteomics, we developed an experimental computational framework, plexDIA, for simultaneously multiplexing analysis both peptides samples. Multiplexed with plexDIA increases multiplicatively number labels without reducing proteome coverage or quantitative accuracy. By using 3-plex nonisobaric mass tags,...
Mass spectrometry (MS) enables specific and accurate quantification of proteins with ever-increasing throughput sensitivity. Maximizing this potential MS requires optimizing data acquisition parameters performing efficient quality control for large datasets. To facilitate these objectives data-independent (DIA), we developed a second version our framework data-driven optimization methods (DO-MS). The DO-MS app v2.0 (do-ms.slavovlab.net) allows one to optimize evaluate results from both...
Single-cell proteomics by mass spectrometry (MS) allows quantifying proteins with high specificity and sensitivity. To increase its throughput, we developed nPOP, a method for parallel preparation of thousands single cells in nanoliter volume droplets deposited on glass slides. Here, describe protocol emphasis flexibility to prepare samples different multiplexed MS methods. An implementation plexDIA demonstrates accurate quantification about 3,000 - 3,700 per human cell. The is implemented...
Einfach gut: Ein effizientes Verfahren, das ausgehend von chiralen Alkoxyamin-Cyclopropanen in ausgezeichneten Ausbeuten zu homochiralen 2,5-cis-Pyrrolidinen führt, wurde bei der Totalsynthese (−)-Allosecurinin genutzt (siehe Schema). Die Synthese liefert die enantiomerenreine Zielverbindung nach 15 Stufen einer Gesamtausbeute 5 %. OTf: Trifluormethansulfonat, Boc: tert-Butoxycarbonyl, PG: Schutzgruppe. Supporting information for this article is available on the WWW under...
Cross‐linked polymeric gels are widely used in applications ranging from biomaterial scaffolds to additives enhanced oil recovery. Despite this, fundamental understanding of the effect polymer concentration and reaction mechanism on scaffold structure is lacking. We measure properties during gelation using multiple particle tracking microrheology. To determine concentration, we as interactions increased backbone precursor solution: below, at above overlap . structural changes due mechanism,...
Eine allgemeine Umlagerung von Nitronen zur Einführung Amidbindungen in einem photochemischen Fließreaktor wird beschrieben (siehe Schema). Einfache Aryl-Alkyl-Amidbindungen ebenso wie komplizierte Peptidbindungen werden effizient binnen weniger als 20 Minuten Verweilzeit erhalten. Nach der Methode wurde ein Tetrapeptid synthetisiert, und auch die Peptidfragmentkupplung war möglich. As a service to our authors and readers, this journal provides supporting information supplied by the authors....