Abstract Background One of the drawbacks currently available vectors for gene therapy is lack selectivity in delivery. We have therefore investigated a strategy to generate immunoliposomes target non‐viral cell surface receptors on endothelium. Materials and methods developed novel method coupling antibodies (Abs) liposomes complexed DNA, using mild heat treatment aggregate immunoglobulin G (IgG). The interaction plasmid Abs was measured gel retardation assay resonant mirror biosensor. size...

Purpose To report outcome, complications and safety of retropupillary fixated iris-claw intraocular lenses in a pediatric population. Design Retrospective study. Patients Methods Ten consecutive patients (15 eyes) underwent placement between October 2007 July 2013 at the Department Ophthalmology, Medical University Graz General Hospital Klagenfurt, Austria. Postoperative visual acuity were analyzed. Results Median final best-corrected improved by 0.12 logMAR from preoperative baseline. Mean...

Extracellular matrix-degrading enzymes are crucial for cancer metastases. One group of that has been increasingly implicated in the breakdown extracellular matrix, and hence intravasation dissemination tumour cells, is family metalloproteinases. In recent past, increasing efforts have led to development more or less specific matrix metalloproteinase (MMP) inhibitors. Data concerning molecular nature timing contribution MMPs spread paramount importance clarifying which MMP an appropriate...

A 49-year-old patient developed pupillary block glaucoma with an intraocular pressure (IOP) of 29 mm hg after implantation a phakic lens (IOL) (NuVita, Bausch & Lomb) in the left eye. anterior chamber deepened again, and iop decreased to 16 neodymium: YAG iridotomy. Pupillary may occur IOL without iridotomy, we advocate that routine iridotomy be performed during surgeries.

Purpose.: Uveitis is a major cause for visual impairment. Inflammation-related gene polymorphisms have previously been shown to confer susceptibility different types of uveitis. Recently, IL-2 receptor alpha (IL2RA, also called CD25) and IL-7 (IL7RA) variants (rs2104286, rs12722489, rs6897932) identified play an essential role in the pathogenesis immune-mediated diseases. Their uveitis, however, has not yet studied. The present study was set investigate hypothesized association these...

To examine the effect of modulating lymphocyte costimulation pathways through CD28 and CD154 (CD40 ligand) in a model corneal allograft rejection, with particular interest changes observed features rejection.CD28 knock-out (CD28KO) wild-type BALB/c control mice received grafts from fully major histocompatibility complex (MHC)-mismatched C3H donors were treated CTLA4-Ig and/or anti-CD154 Ab on days 0, 2, 4 after transplantation. Proliferation CD28KO T cells response to stimulators was...

Tyrosinase-based reverse transcriptase-polymerase chain reaction (RT-PCR) is a method for the detection of circulating melanoma cells in peripheral blood. To our knowledge, no long-term studies on prognostic impact tyrosinase PCR uveal have yet been reported. In this prospective, non-randomized, observational cohort study, we included 41 patients with malignant melanoma. RT-PCR was performed each patient before and after treatment. A clinical follow-up at least 5 years, including chest...

Purpose: Evaluation of mRNA and microRNA (miRNA) expression in epithelium stroma patients with keratoconus. Methods: The eight corneas keratoconus non-keratoconus healthy controls were studied separately. RNA was extracted, miRNA analyses performed using microarrays. Differentially expressed mRNAs miRNAs epithelial stromal samples compared to identified. Selected genes further validated RT-qPCR. Results: We discovered 170 1498 deregulated protein-coding KC samples. In addition, 180 379...

To investigate the distribution of somatostatin receptor (SSR) subtypes 2, 3, and 5 in uveal melanomas their diagnostic possible therapeutic value.SSRs were investigated 25 paraffin-embedded eyes with 6 normal without any disease, by using polyclonal antiserum directed to SSR2A, -2B, -3, -5. Antigen expression was evaluated a semiquantitative method. The pattern SSR correlated patients' ad vitam prognosis use Kaplan-Meier survival curve. Six different human melanoma cell lines incubated...

We report a case of sterile keratitis after Ferrara intracorneal ring (Ferrara Ophthalmics) implantation into the right cornea keratoconus patient who also suffered from atopic blepharoconjunctivitis. The resolved within few days removal rings. Ocular atopy with should be considered in differential diagnosis insertion segments, which can easily treated by removal.

<h3>Aim</h3> To evaluate visual quality and postoperative results as well adverse events in myopic patients undergoing I-CARE anterior-chamber angle-supported phakic intraocular lens (IOL) implantation. <h3>Design</h3> A retrospective, non-randomised, case series. <h3>Participants</h3> Data on 29 eyes (16 patients) receiving IOL for high myopia (–11.66±3.3) were analysed. <h3>Methods</h3> The IOLs implanted between 2003 2006 at the Department of Ophthalmology, Medical University, Graz,...

Purpose: The aim was to produce 2 tissue microarrays (TMAs) for keratoconus (KC) corneas and evaluate the expression of stress-related markers, epidermal growth factor receptor (EGFR), 8-oxo-2′-deoxyguanosine (8-OHdG), in KC corneas. Methods: corneal buttons 66 patients with were included both TMAs; 10 Fuchs endothelial dystrophy (FECD), 20 normal autopsy corneas, 32 nonocular cores served as controls. immunolabeling EGFR 8-OHdG compared those controls by TMAJ software using an H-score...

Polymorphisms of the vitamin D metabolism gene CYP27B1 showed associations with multiple autoimmune diseases. The aim this study was to investigate a possible association between rs703842 A>G polymorphism and HLA-B27-associated uveitis.One hundred fifty-nine patients uveitis, 138 HLA-B27-negative controls 100 HLA-B27-positive were recruited for retrospective case-control study. Main outcome parameters genotype distribution allelic frequencies determined by polymerase chain reaction.Carriers...