Alex Fiori da Silva

ORCID: 0000-0001-6266-8813
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About
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Research Areas
  • Photodynamic Therapy Research Studies
  • Nanoplatforms for cancer theranostics
  • Essential Oils and Antimicrobial Activity
  • Antimicrobial agents and applications
  • Bacterial biofilms and quorum sensing
  • Advanced Chemical Sensor Technologies
  • Listeria monocytogenes in Food Safety
  • bioluminescence and chemiluminescence research
  • Biosensors and Analytical Detection
  • Skin Protection and Aging
  • Antimicrobial Peptides and Activities
  • Medicinal Plants and Neuroprotection
  • Coconut Research and Applications
  • Legionella and Acanthamoeba research
  • Microplastics and Plastic Pollution
  • Photoacoustic and Ultrasonic Imaging
  • Nanocomposite Films for Food Packaging
  • Insect Pest Control Strategies
  • Environmental and biological studies
  • Agricultural and Food Sciences
  • Cassava research and cyanide
  • Phytochemicals and Antioxidant Activities
  • Biochemical Analysis and Sensing Techniques
  • Education during COVID-19 pandemic
  • Ocular Infections and Treatments

Universidade Estadual de Maringá
2014-2023

Universidade do Estado de Minas Gerais
2020-2023

Instituto Federal de Educação Ciência e Tecnologia do Paraná
2020

Instituto Federal de Educação, Ciência e Tecnologia do Pará
2020

Universidade do Porto
2018

Blücher (Germany)
2014

The thermal and chemical-based methods applied for microbial control in the food industry are not always environmentally friendly may change nutritional organoleptic characteristics of final products. Moreover, efficacy sanitizing agents be reduced when cells enclosed biofilms. objective this study was to investigate effect photodynamic inactivation, using two xanthene dyes (rose bengal erythrosine) as photosensitizing green LED a light source, against Staphylococcus aureus, Listeria...

10.3390/molecules23092288 article EN cc-by Molecules 2018-09-07

The present study evaluated the antibacterial and antibiofilm activity of carvacrol against Salmonella Typhimurium. minimum inhibitory concentration (MIC) bactericidal (MBC) were determined time-kill curve scanning electron microscopy (SEM) performed to evaluate activity. Antibiofilm was by quantifying total biomass using crystal violet assay, metabolic MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. action preformed biofilm on polypropylene stainless steel also...

10.1590/s2175-97902018000117229 article EN cc-by Brazilian Journal of Pharmaceutical Sciences 2018-06-07

Summary The present study evaluated the effects of carvacrol and thymol against Salmonella spp. biofilm on polypropylene. efficacy compounds was assessed by quantifying cells during after formation polypropylene performing scanning electron microscopy. During formation, thymol, at subinhibitory concentrations, reduced bacterial counts about 1–2 log, while established biofilms were 1–5 log MIC or 2× MIC. greatest reduction in carvacrol‐treated biofilms, 5 observed with 156 312 μg mL −1 (MIC...

10.1111/ijfs.12934 article EN International Journal of Food Science & Technology 2015-08-25

Abstract This study evaluated the rose bengal‐ and erythrosine‐mediated photoinactivation against Salmonella Typhimurium Staphylococcus aureus planktonic sessile cells using green LED as a light source. The free‐living or 2‐day‐old biofilm were treated with different concentrations of photosensitizing agents subjected to irradiation. Only 5 min photosensitization bengal at 25 nmol L −1 75 μ mol completely eliminated S. S . cells, respectively. Erythrosine 500 exposure also reduced...

10.1111/php.13104 article EN Photochemistry and Photobiology 2019-03-29

Salmonella spp.are a major cause of foodborne illness throughout the world (World Health Organization, 2013), attracting considerable concern for public health and food industry.According to Centers Disease Control Prevention ( approximately 40,000 new cases salmonellosis are reported every year in United States, with an estimated 400 deaths.Salmonella biofilm has important economic consequences because it can be potential source contamination (Steenackers et al., 2012).Recently, essential...

10.2323/jgam.60.119 article EN The Journal of General and Applied Microbiology 2014-01-01

The objective of this study was to evaluate the antimicrobial effectiveness cinnamaldehyde (CIN) and potassium sorbate (P.S.), alone in combination, against Salmonella Typhimurium Staphylococcus aureus vitro apple jam. Antimicrobial activity investigated by minimum inhibitory concentration (MIC) bactericidal (MBC), time-kill assay determination fractional index. CIN MIC MBC 312 μg/mL. P.S. were 2500 5000 μg/mL, respectively, S. Typhimurium; 10,000 20,000 aureus. compounds combined exhibited...

10.1177/10820132231165541 article EN Food Science and Technology International 2023-03-27

Photodynamic antimicrobial chemotherapy (PAC) is an efficient tool for inactivating microorganisms. This technique a good approach to inactivate the foodborne microorganisms, which are responsible one of major public health concerns worldwide—the diseases. In this work, response surface methodology (RSM) was used evaluate interaction Eosin Y (EOS) concentration and irradiation time on Staphylococcus aureus counts sequence designed experiments model combined effect each factor response. A...

10.3390/antibiotics9030125 article EN cc-by Antibiotics 2020-03-17

Abstract In this work, the photodynamic bactericidal effect of Rose Bengal (RB), combined with green LED light, against Staphylococcus aureus and Salmonella Typhimurium, was investigated. The interaction RB concentration illumination time evaluated using a response surface methodology, second‐order polynomial empirical model adjusted to experimental data. derived predicted influences these factors on S. S . Typhimurium counts, in accordance predictions observations ( R 2 = 0.8483 p .0013 for...

10.1111/jfs.12736 article EN Journal of Food Safety 2019-11-19

Carvacrol presents action in Salmonella Typhimurium biofilms, however the antibiofilm mechanism of this compound has not been fully established yet. In present study, aim was to evaluate protein profile changes S. biofilm treated with carvacrol. Proteomic analysis versus untreated showed several proteins involved and antioxidant activity. The DsbA (thiol: disulfide interchange DsbA), LuxS (S-ribosylhomocysteine lyase), DksA (RNA polymerase binding transcription factor DksA), SODs (superoxide...

10.32725/jab.2020.014 article EN cc-by-nc-nd Journal of Applied Biomedicine 2020-09-22

Abstract Staphylococcus aureus is one of the main etiological agents causing foodborne diseases, and development new antibacterial urgent. This study evaluated activity possible mechanism action 1,3,4-oxadiazole LMM6 against S. aureus. The minimum inhibitory concentration (MIC) ranged from 1.95 to 7.81 µg ml−1. time-kill assay showed that 48-h treatment at 1× 8× MIC reduced by 4 log colony forming unit (CFU), indicating a bacteriostatic effect. Regarding LMM6, there was accumulation reactive...

10.1093/lambio/ovad138 article EN Letters in Applied Microbiology 2023-12-09

As enfermidades de origem alimentar não causam somente transtornos à saúde e bem estar dos indivíduos afetados, mas também se associam a graves consequências econômicas para sociedade. Staphylococcus aureus é um patógeno grande importância, pois frequentemente responsável por intoxicações alimentares. O presente trabalho avaliou o efeito da Inativação Fotodinâmica utilizando como Fotossensibilizadores (FS’s), Eritrosina (ERI) seus derivados ésteres metil (ERIMET) butil (ERIBUT) frente...

10.5151/foodsci-microal-343 article PT 2014-11-01

Pseudomonas aeruginosa é uma bactéria deteriorante e patogênica comumente encontrada no ambiente estabelecimentos processadores de alimentos, sendo associado à deterioração grande variedade alimentos. Devido a sua resistência aos agentes antimicrobianos, novas tecnologias estão estudadas para o seu controle. A inativação fotodinâmica abordagem promissora que causa danos micro-organismos na presença um fotossensibilizador (FS) ou corante atóxico luz em comprimento onda adequado. O presente...

10.5151/foodsci-microal-344 article PT cc-by 2014-11-01

O presente trabalho abordou, a partir da revisão bibliográfica, os empecilhos e desafios na doação de sangue por pessoas LGBTQIAP+ à luz decisão do Supremo Tribunal Federal, relacionados garantia princípio dignidade pessoa humana. Historicamente, essa população tem enfrentado restrições discriminações no contexto sangue, baseadas em estereótipos discriminatórios. No entanto, STF Ação Direta Inconstitucionalidade 5543/DF reconheceu inconstitucionalidade das impostas pelo Ministério Saúde,...

10.9771/rds.v4i1.51859 article PT cc-by-nc-nd Revista Direito e Sexualidade 2023-06-30
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