A5002111331- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Gut microbiota and health
- Wastewater Treatment and Nitrogen Removal
- Bacteriophages and microbial interactions
- Forest Ecology and Biodiversity Studies
- Protist diversity and phylogeny
- Bacterial biofilms and quorum sensing
- Methane Hydrates and Related Phenomena
- Mentoring and Academic Development
- bioluminescence and chemiluminescence research
- Medical Imaging and Pathology Studies
- Microbial infections and disease research
- Spaceflight effects on biology
- Viral gastroenteritis research and epidemiology
- Dark Matter and Cosmic Phenomena
- Coastal wetland ecosystem dynamics
- Cell Image Analysis Techniques
- Health and Medical Research Impacts
- Evolution and Genetic Dynamics
- Interprofessional Education and Collaboration
- Career Development and Diversity
- Microbial Natural Products and Biosynthesis
- Space Science and Extraterrestrial Life
- Mycorrhizal Fungi and Plant Interactions
Wageningen University & Research
2020-2024
Friedrich Schiller University Jena
2017-2022
Netherlands Institute of Ecology
2018
Despite the widely observed predominance of Cand. Patescibacteria in subsurface communities, their input source and ecophysiology are poorly understood. Here we study mechanisms formation a groundwater microbiome subsequent differentiation Patescibacteria. In Hainich Critical Zone Exploratory, Germany, trace microorganisms from forested soils preferential recharge areas through fractured aquifers along 5.4 km hillslope well transect. were preferentially mobilized constituted 66%...
Near-surface groundwaters are prone to receive (in)organic matter input from their recharge areas and known harbor autotrophic microbial communities linked nitrogen sulfur metabolism. Here, we use multi-omic profiling gain holistic insights into the turnover of inorganic compounds, carbon fixation processes, organic processing in groundwater. We sampled biomass two superimposed aquifers via monitoring wells that follow groundwater flow its area through differences hydrogeochemical settings...
Mentor relationships are crucial to retention, success, and wellbeing of women underrepresented minority scientists in academia. A network diverse mentors may support achieving long-term career goals, advancement, retention both mentees, thus enhancing diversity, equity, inclusion initiatives.
Microbial activity is key in understanding the contribution of microbial communities to ecosystem functions. Metabolic labelling with heavy water (D2 O) leads formation carbon-deuterium bonds active microorganisms. We illustrated how D2 O allows monitoring metabolic combined a functional characterization populations complex communities. First, we demonstrated by single cell Raman microspectroscopy that all measured bacterial cells from groundwater isolates growing medium were labelled. Next,...
Summary Assembling microbial and viral genomes from metagenomes is a powerful appealing method to understand structure–function relationships in complex environments. To compare the recovery of microorganisms their viruses groundwater, we generated shotgun with Illumina sequencing accompanied by long reads derived Oxford Nanopore Technologies (ONT) platform. Assembly metagenome‐assembled genome (MAG) metrics for both microbes were determined an Illumina‐only assembly, ONT‐only hybrid...
The discovery of the Archaea is a major scientific hallmark twentieth century. Since then, important features their cell biology, physiology, ecology, and diversity have been revealed. Over course some 40 years, known archaea has expanded from 2 to about 30 phyla comprising over 20,000 species. Most this archaeal revealed by environmental 16S rRNA gene amplicon sequencing surveys using broad range universal targeted primers. Of few primers that target large fraction diversity, all display...
Abstract Microbial communities are not only shaped by the diversity of microorganisms and their individual metabolic potential, but also vast amount intra- interspecies interactions that can occur pairwise among microorganisms, we suggest more attention should be drawn towards effects on entire microbiome emerge from between community members. The production certain metabolites tied to a specific microbe-microbe interaction might subsequently influence physicochemical parameters habitat,...
Despite the high relevance of anaerobic ammonium oxidation (anammox) for nitrogen loss from marine systems, its relative importance compared to denitrification has less been studied in freshwater ecosystems, and our knowledge is especially scarce groundwater. Surprisingly, phospholipid fatty acids (PLFA)-based studies identified zones with potentially active anammox bacteria within two superimposed pristine limestone aquifer assemblages Hainich Critical Zone Exploratory (CZE; Germany). We...
Abstract The phyllosphere is a challenging microbial habitat in which microorganisms can flourish on organic carbon released by plant leaves but are also exposed to harsh environmental conditions. Here, we assessed the relative importance of canopy position—top, mid, and bottom at height between 31 20 m—and tree species identity for shaping microbiome floodplain hardwood forest. Leaf material was sampled from three species—maple ( Acer pseudoplatanus L.), oak Quercus robur linden Tilia...
Groundwater ecosystems face the challenge of energy limitation due to absence light-driven primary production. Lack space and low oxygen availability might further contribute generally assumed food web complexity. Chemolithoautotrophy provides additional input carbon within subsurface, however, we still do not understand how abundances chemolithoautotrophs, differences in surface input, control subsurface Using a molecular approach, aimed disentangle different levels potential trophic...
Subsurface ecosystems like groundwater harbour diverse microbial communities, including small-sized, putatively symbiotic organisms of the Candidate Phyla Radiation, yet little is known about their ecological preferences and potential partners. Here, we investigated a member superphylum Microgenomates (Cand. Roizmanbacterium ADI133) from oligotrophic using mini-metagenomics monitored its spatio-temporal distribution 16S rRNA gene analyses. A Roizmanbacteria-specific quantitative PCR assay...
Subsurface microbiomes provide essential ecosystem services, like the generation of drinking water. These ecosystems are devoid light-driven primary production, and microbial life is adapted to resulting oligotrophic conditions.
Microorganisms can produce a plethora of secondary metabolites, some acting as signaling compounds and others suppressing agents. As yet, the potential groundwater microbes to antimicrobial increase their competitiveness against other bacteria has not been examined. In this study, we developed an AlamarBlue® based high-throughput screening method that allowed for fast highly standardized evaluation both growth-inhibiting -promoting metabolites. With technique, 149 screened bacterial isolates...
Abstract The phyllosphere is a challenging microbial habitat in which microorganisms can flourish on organic carbon released by plant leaves but are also exposed to harsh environmental conditions. Here, we assessed the relative importance of canopy position – top, mid, and bottom at height between 31 m 20 tree species identity for shaping microbiome floodplain hardwood forest. Leaf material was sampled from three - maple ( Acer pseudoplatanus L. ), oak Quercus robur lime Tilia cordata MILL....
Abstract Assembling microbial and phage genomes from metagenomes is a powerful appealing method to understand structure-function relationships in complex environments. In order compare the recovery of microorganisms their phages groundwater, we generated shotgun with Illumina sequencing accompanied by long reads derived Oxford Nanopore platform. Assembly metagenome-assembled genome (MAG) metrics for both microbes viruses were determined Illumina-only assemblies hybrid assembly approach....
Effective mentoring implies a two-way relationship in which mentees obtain benefits from the knowledge and training provided by mentors, mentors gain possibility of contemplating learning new perspectives, self-evaluating their roles and, consequence, growing as professionals. Mentorship relationships cannot be separated cultural societal backgrounds. Thus, they often reflect systemic biases requiring active effort to counteract institutional inequities. Such efforts, particularly when...