A5036497223- Mitochondrial Function and Pathology
- Advanced Electron Microscopy Techniques and Applications
- ATP Synthase and ATPases Research
- Electron and X-Ray Spectroscopy Techniques
- Renal and related cancers
- Cytomegalovirus and herpesvirus research
- Photosynthetic Processes and Mechanisms
- Metabolism and Genetic Disorders
- Mosquito-borne diseases and control
- Infectious Encephalopathies and Encephalitis
- Enzyme Structure and Function
- Advanced Materials Characterization Techniques
- Cell Image Analysis Techniques
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
Scripps Research Institute
2021-2024
Scripps Institution of Oceanography
2021-2022
Scripps (United States)
2021
Cellular cryo-electron tomography (cryo-ET) enables three-dimensional reconstructions of organelles in their native cellular environment at subnanometer resolution. However, quantifying ultrastructural features pleomorphic three dimensions is challenging, as defining the significance observed changes induced by specific perturbations. To address this challenge, we established a semiautomated workflow to segment organellar membranes and reconstruct underlying surface geometry cryo-ET....
CellPAINT is an interactive digital tool that allows non-expert users to create illustrations of the molecular structure cells and viruses. We present a new release with several key enhancements, including ability generate custom ingredients from information in Protein Data Bank, interaction, grouping, locking functions streamline creation assemblies illustration large, complex scenes. An example as for hypothesis generation interpretation cryoelectron tomograms presented. freely available...
ABSTRACT Cellular cryo-electron tomography (cryo-ET) enables 3-dimensional reconstructions of organelles in their native cellular environment at subnanometer resolution. However, quantifying ultrastructural features pleomorphic three dimensions is challenging, as defining the significance observed changes induced by specific perturbations. To address this challenge, we established a semi-automated workflow to segment organellar membranes and reconstruct underlying surface geometry cryo-ET....
Maternally inherited mitochondrial DNA (mtDNA) encodes essential subunits of the oxidative phosphorylation system, but is also a major damage-associated molecular pattern (DAMP) that engages innate immune sensors when released into cytoplasm, outside cells or circulation 1 . This function mtDNA contributes to antiviral resistance, unfortunately causes pathogenic inflammation in many disease contexts 2 Cells experiencing stress due depletion mtDNA-packaging protein, Transcription Factor A,...
Abstract Mitochondrial fission is required for proper segregation during cell division, quality control, and cellular homeostasis (metabolism energy production). Despite its importance, models of the process remain speculative. Here we apply cryogenic electron tomography to image nanoscale architecture mitochondrial in mammalian cells. We find that constriction inner outer membranes coordinated, suggesting force on both applied externally. While observe ER at sites, it did not encircle...