A5073458713- Ion channel regulation and function
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Cardiomyopathy and Myosin Studies
- S100 Proteins and Annexins
- Adipose Tissue and Metabolism
- Cellular Mechanics and Interactions
- Photoreceptor and optogenetics research
- Molecular Sensors and Ion Detection
- Calcium signaling and nucleotide metabolism
- Cardiovascular Effects of Exercise
- Muscle Physiology and Disorders
- ATP Synthase and ATPases Research
- Protein Structure and Dynamics
- Luminescence and Fluorescent Materials
- Synthesis and Reactivity of Heterocycles
- Vitamin K Research Studies
- Liver Disease and Transplantation
- Force Microscopy Techniques and Applications
- Anesthesia and Neurotoxicity Research
- Computational Drug Discovery Methods
- Nitric Oxide and Endothelin Effects
- Lipid Membrane Structure and Behavior
Polish Academy of Sciences
2007-2025
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2025
Warsaw University of Life Sciences
2012
St Thomas' Hospital
1998
Novartis (Switzerland)
1992-1993
alpha B-Crystallin is a 20-kd peptide highly homologous to the small heat-shock proteins. This protein forms soluble homomultimeric complexes (M(r), 300-700 kd) and very abundant in cardiac muscle cells. In vitro experiments (affinity column chromatography binding studies with isolated proteins) have shown that B-crystallin interacts directly actin and, particular, desmin filaments. The immunocytochemical localization of within cardiomyocytes showed distributed exclusively central region I...
Background: The nitric oxide–sensitive guanylyl cyclase/cGMP-dependent protein kinase type I signaling pathway can afford protection against the ischemia/reperfusion injury that occurs during myocardial infarction. Reportedly, voltage and Ca 2+ -activated K + channels of BK are stimulated by cGMP/cGMP-dependent I, recent ex vivo studies implicated increased activity favors survival myocardium at ischemia/reperfusion. It remains unclear, however, whether molecular events downstream cGMP...
A new straightforward methodology for the synthesis of phosphonium salts integrated with π-conjugated scaffold from phosphine oxides has been developed. It is now possible to obtain cyclic possessing up eight conjugated rings and bearing e.g. pyrrole, thiophene, indole or benzofuran scaffolds abundant commercially available materials in high yields. An enticing feature this general strategy that one-step procedure typically does not require chromatographical purification. Still greater...
1 Changes in cytosolic [Ca2+] ([Ca2+]i) were measured isolated rat trabeculae that had been micro-injected with fura-2 salt, order to investigate the mechanism by which twitch force changes following an alteration of muscle length. 2 A step increase length produced a rapid potentiation but not Ca2+ transient. The rise was unaffected inhibiting sarcoplasmic reticulum (SR) ryanodine and cyclopiazonic acid. 3 force-[Ca2+]i relationship myofibrils situ, determined from twitches tetanic...
Abstract Brain edema in acute hepatic encephalopathy (HE) is due mainly to swelling of astrocytes. Efflux potassium implicated the prevention glial under hypoosmotic conditions. We investigated whether pathogenic factors HE, glutamine (Gln) and/or ammonia, induce alterations expression channels (Kir4.1, Kir2.1) and Na + ‐K ‐2Cl − cotransporter‐1 (NKCC1) rat cerebral cortex cultured cortical astrocytes these have consequences for efflux astrocytic swelling. Thioacetamide‐induced liver failure...
Abstract The membrane‐intrinsic protein phospholamban (PLN), the regulatory of sarcoplasmic reticulum (SR) Ca 2+ ‐ATPase, was chemically synthesized. synthesis accomplished by double couplings and efficient capping procedures, thus eliminating hydrophobic failure sequences. crude peptide purified high‐performance liquid chromatographic ion exchange gel permeation chromatography in chloroform–methanol mixtures. Ion spray mass spectroscopy showed that product had correct molecular mass. Sodium...
The controlled hydrolysis of sulfone-rhodamines affords a series core-modified red-emitting rhodols, the fluorescence which is sensitive to solvent polarity. After equipping with PPh<sub>3</sub><sup>+</sup> group they selectively stain mitochondria.
Neutral rhodol-based red emitters are shown to efficiently localize in mitochondria, as demonstrated by confocal microscopy and co-localization studies.
Blue to yellow-emitting 2-oxo-pyrano[2,3- b ]indolizines, which are efficiently prepared in two steps from pyridinium salts, have strong emission across the solvent polarity scale.
Annexin VI is a 68‐kDa calcium‐, phospholipid‐, and cytoskeletal‐element‐binding protein, which has been implicated in various processes, including calcium release sequestration calcifying cartilage, receptor‐mediated endocytosis human fibroblasts, secretion from chromaffin granules. In these processes it was found that, addition to Ca 2+ annexin, the presence of ATP also prerequisite. present report we show that annexin binds binding nucleotide protein accompanied by quenching an intrinsic...