A5021568228- Ion channel regulation and function
- Nanopore and Nanochannel Transport Studies
- Protein Structure and Dynamics
- Neuroscience and Neuropharmacology Research
- Atherosclerosis and Cardiovascular Diseases
- Cardiac electrophysiology and arrhythmias
- Enzyme Structure and Function
- Monoclonal and Polyclonal Antibodies Research
- Protein Kinase Regulation and GTPase Signaling
- Bacterial Genetics and Biotechnology
- Cholesterol and Lipid Metabolism
- Chronic Lymphocytic Leukemia Research
- Adipokines, Inflammation, and Metabolic Diseases
- Molecular Sensors and Ion Detection
- Immune Cell Function and Interaction
- Photochromic and Fluorescence Chemistry
- Advanced NMR Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Antibiotic Resistance in Bacteria
- RNA and protein synthesis mechanisms
- Analytical Chemistry and Sensors
- Lipoproteins and Cardiovascular Health
- X-ray Spectroscopy and Fluorescence Analysis
University of Chicago
2010-2022
University of Illinois Chicago
2010-2022
Boston University
2001
An approach combining small-angle X-ray solution scattering (SAXS) data with coarse-grained (CG) simulations is developed to characterize the assembly states of Hck, a member Src-family kinases, under various conditions in solution. First, basis set comprising small number generated from extensive CG simulations. Second, theoretical SAXS profile for each state computed by using Fast-SAXS method. Finally, relative population different determined via Bayesian-based Monte Carlo procedure...
Abstract —To determine whether T cells and B influence lipid metabolism atherosclerosis, we crossed apolipoprotein E–deficient (apoE°) mice with recombination activating gene 2–deficient (RAG2°) mice. Total plasma cholesterol levels were ≈20% higher in male apoE° compared the apoE°RAG2° at 8 weeks of age, triglyceride 2.5-fold even when similar. Male between 400 600 mg/dL age euthanized 27 40 age. The aortic root lesion area mice, that immune-competent was 81% 57% smaller respectively. In...
Objective— We sought to examine whether there is a site-specific effect on atherosclerosis of the absence mature T and B cells caused by recombination activating-gene deficiency in LDL receptor-deficient mice this influence extent backcrossing C57BL/6 mice. Methods Results— Male were fed atherogenic diets for 3 months. In strain 1 mice, which ≈93% genes from led significant reduction both aortic sinus innominate artery. 2 ≈99+% immune system atherosclerosis, with but not artery, similar...
Abstract C-type inactivation is of great physiological importance in voltage-activated K + channels (Kv), but its structural basis remains unresolved. Knowledge about has been largely deduced from the bacterial channel KcsA, whose selectivity filter constricts under inactivating conditions. However, highly sensitive to molecular environment, which different Kv than KcsA. In particular, a glutamic acid residue at position 71 along pore helix KcsA substituted by valine conserved most channels,...
OmpF, a multiionic porin from Escherichia coli, is useful protypical model system for addressing general questions about electrostatic interactions in the confinement of an aqueous molecular pore. Here, favorable anion locations OmpF pore were mapped by anomalous X-ray scattering Br– ions four different crystal structures and compared with Mg2+ sites Rb+ previous diffraction study to provide complete picture cation transfer paths along channel. By comparing various crystallization...
This study tests the hypothesis that autoantibodies to oxidation epitopes on oxidized LDL (OxLDL) promote clearance of OxLDL from plasma. Human (hLDL) was injected into immune-competent apolipoprotein E-deficient (apoE−/−) mice and immune-deficient apoE−/−/recombination-activating gene-deficient lack mature T B cells thus antibodies. There a progressive decrease in human apoB-100 plasma all mice, but rate not greater than mice. Interestingly, phospholipid (OxPL) as detected by EO6 antibody...
In voltage-gated K+ channels (Kv), membrane depolarization promotes a structural reorganization of each the four voltage sensor domains surrounding conducting pore, inducing its opening. Although crystal structure Kv1.2 provided first atomic resolution view eukaryotic Kv channel, several components sensors remain poorly resolved. particular, position and orientation charged arginine side chains in S4 transmembrane segments controversial. Here we investigate proximity pore domain functional...
The selectivity filter is an essential functional element of K+ channels that highly conserved both in terms its primary sequence and three-dimensional structure. Here, we investigate the properties ion channel from Gram-positive bacterium Tsukamurella paurometabola with a formed by uncommon proline-rich sequence. Electrophysiological recordings show it non-selective cation activity depends on Ca2+ concentration. In crystal structure, adopts novel conformation ions bound within near pore...
We have used adenovirus-mediated gene transfer in apoA-I-deficient (A-I-/-) mice to probe the vivo assembly and metabolism of HDL using apoA-I variants, focusing primarily on role C-terminal 32 amino acids (helices 9-10). Lipid, lipoprotein, analyses showed that plasma levels mutants were 40-88% lower than wild type (WT) human despite comparable expression liver. WT mutant 1 (P165A, E172A) formed spherical particles with size density HDL2 HDL3. Mutant 2 (E234A, E235A, K238A, K239A) generated...
To examine the association of apolipoprotein (apo) E with nascent hepatic lipoproteins we have prepared stable transfectants rat hepatoma cell line McA RH7777 expressing human apoE3 cDNA. When secreted from control cells were separated on fast protein liquid chromatography (FPLC) columns, apoE was detected in very low density (VLDL) and high lipoprotein (HDL) fractions, while apoA-I found HDL free fractions. Human also associated VLDL particles transfected cells. Expression resulted a...
Engineered antibody fragments (Fabs) have made major impacts on structural biology research, particularly to aid determination of membrane proteins. Nonetheless, Fabs generated by traditional monoclonal technology suffer from challenges routine production and storage. Starting the known IgG paratopes an that binds “turret loop” KcsA K+ channel, we engineered a synthetic Fab (sFab) based upon highly stable Herceptin scaffold, which can be recombinantly expressed in Escherichia coli purified...
Barium is a potent blocker of the KcsA potassium channel. A strategy using x-ray crystallography and molecular dynamics (MD) simulation has been used to understand this phenomenon as described in Rohaim et al. [1]. Wild type purified homogeneity crystallized low high K+ conditions. Crystals are grown hanging drop vapor diffusion method. To examine barium binding selectivity filter KcsA, crystals systemically soaked various concentrations chloride solution. X-ray datasets collected at...