The ABC protein P-glycoprotein (Pgp or ABCB1) is a multidrug efflux pump capable of transporting many structurally diverse substrates from within the lipid bilayer. Previous studies have demonstrated importance membrane in modulating Pgp function, but few quantified these effects. We employed purified reconstituted into phospholipid bilayers with defined gel to liquid-crystalline melting transitions investigate effect environment on transporter and three its substrates. Equilibrium dialysis...

<title>Abstract</title> <bold>Background: </bold>Malnutrition and poor dietary intake are major health challenges today.^1,2,3,4,5 There well-established benefits of nutrition interventions, but a lack formalized training in medical school residency.^3,6,7,8 is also little published information regarding impact on residents. Physicians knowledge, skills, confidence, to effectively counsel daily practice.^{1,3,9,10,11,12} Consequently, there urgent need improve...

The ABC transporter P-glycoprotein (Pgp, ABCB1) actively exports structurally diverse substrates from within the lipid bilayer, leading to multidrug resistance. Many aspects of Pgp function are altered by phospholipid environment, but its interactions with sterols remain enigmatic. In this work, functional interaction between purified and various was investigated in detergent solution proteoliposomes. Fluorescence studies showed that dehydroergosterol, cholestatrienol, NBD-cholesterol...

A new theory is presented to explain the conductivity maxima of molten salts (versus temperature and pressure). In theory, due ions hopping from counterion counterion, its dependence can be explained with an ordinary Arrhenius equation in which frequency prefactor (for opportunities) activation energy E(a) hopping) are density dependent. The maximum competing effects: as decreases, opportunities for increases, but probability that opportunity successfully hopped decreases rising caused by...

The COVID-19 pandemic prompted the rapid uptake of Virtual Care (VC). Positive patient outcomes with VC are previously reported but little is known about experiences patients and providers using during pandemic. We aimed to describe primary care provider experiences, satisfaction, perceptions, attitudes that might explain adoption across continuum inform sustained uptake. conducted a sequential explanatory mixed methods study online surveys virtual interviews convenience sample in Canadian...

Abstract Background Many patients present to their family medicine clinic with more than one health concern, placing an increased demand on physicians. Research into the average number of concerns per regular visit is limited. Recognition frequency that physicians address concern and adapting practices accordingly important for improving patient care. Objective To examine whether routinely multiple different during a single if this influenced by demographics. Methods This study was conducted...

Early detection of malnutrition in hospitalized patients is paramount importance. As poor food intake a marker risk, simple and accurate method to monitor valuable. This quality assurance project aimed determine if service workers (FSW) were able provide estimates patient intakes through visually assessing meal trays at an acute care hospital. FSW conducted visual after meals using the plate pictorial rating scale adapted from My Meal Intake Tool translated their into one 5 consumption...

Chronic diseases disproportionately affect indigenous people in Saskatchewan, with high rates of HIV, hepatitis C, and diabetes. Concentration health care services urban centers minimal in-community service delays access to diagnostic testing treatment for those residing

Obesity is recognized as an independent risk factor for chronic kidney disease through multiple direct and indirect biological pathways. Bariatric surgery a proven, effective method sustained weight loss. However, there relative paucity of data on the impact bariatric renal outcomes.The primary objective was to evaluate change in urine albumin/creatinine ratio (ACR) patients undergoing surgery, at 12 months after procedure. Secondary objectives were determine changes ACR (6 24 months),...

Sexually transmitted infections (STIs) are common in young adults Canada and their prevalence is rising. Assessing sexual health knowledge among essential for developing effective STI education strategies. However, there limited research on the of Canadian university athletes, who have increased risks STIs.

Introduction: Up to 98% of practicing family physicians, and over 75% resident physicians in Canada experience abusive incidents. Despite the negative consequences incidents, few residents report these events their supervisors or institution. We sought estimate prevalence incidents experienced witnessed by Saskatchewan medicine (FMRs) identify responses events. Methods: Anonymous survey invitations were emailed all 110 FMRs November December 2020. Demographic characteristics, frequency...

People who are transgender and gender diverse (TGD) report suboptimal care from health providers. A cross-sectional survey was codesigned with community partners to assess the knowledge, comfort, skills of family physicians, medicine residents, nurse practitioners working TGD patients in Saskatchewan. It administered August October 2019. Of 188 participants, 30% 96% were comfortable providing transition-related non-transition-related medical TGD, respectively. Interest further training...

Key Practitioner Messages Many human trafficking victims access healthcare, but most are not identified. Healthcare providers' overall knowledge in identifying commercially sexually exploited children was strong. providers lack confidence and managing children.

Background: Goals of care discussions ensure patients receive the that they want. Recent studies have recognized opportunity for allied health professionals, such as nurses, in facilitating goals discussions. However, outcomes interventions are not well studied. Aim: To compare led by physicians and nurses. Design: This is a retrospective cohort study admitted to an Internal Medicine unit from January 2018 August 2019. A comprehensive chart review was performed on random sample patients....

Advance care plans (ACPs) increase patient-centered care, reduce caregiver burden and healthcare costs. However, only about 25% of Canadians over 50 years age have completed an ACP. An anonymous, cross-sectional survey was distributed to those ≥70 attending a primary clinic-based COVID-19 vaccination event in Regina, SK April 2021. The included questions demographics, attitudes, engagement ACPs according the Stage Change model, patient-reported influence on ACP thoughts or actions. One...

ABSTRACTRésumé Objectives: Recreational cannabis use was legalized in Canada 2018. There is minimal research assessing family physicians’ abilities to address recreational with patients. We sought assess physician's knowledge and practice of screening counseling patients on consumption. Methods: Family physicians Saskatchewan were invited complete a cross-sectional survey, distributed by the Medical Association, January February 2020. The survey captured practices continued training needs....

Abstract Objectives To evaluate the number of out-of-hospital cardiac arrest (OHCA) patients eligible for extracorporeal cardiopulmonary resuscitation (ECPR) in Saskatchewan and their clinical outcomes, including survival neurological outcomes at discharge. ECPR eligibility was assessed, using criteria from University British Columbia (UBC, Canada), Michigan (UM, United States), California (UC, States) a restrictive criteria. Results We performed retrospective cohort study 200 OHCA (August...

The methadone dose required to prevent withdrawal symptoms in chronic users varies dramatically from 5 300 mg/day, which could be attributed a number of biochemical factors, including human µ-opioid receptor (hMOR) density, down-stream desensitization, concurrent drug use or P-glycoprotein (P-gp) expression, other, possibly genetic factors. Here we describe some changes patients taking higher versus lower doses maintenance therapy (MMT). hMOR density was measured leukocytes...

Multidrug resistance in many human cancers is linked to expression of the P‐glycoprotein multidrug transporter (Pgp, ABCB1), an ABC protein that exports structurally diverse compounds from cells. Since Pgp pumps lipophilic substrates out membrane, lipid environment plays important role regulating its activity. Using purified functionally reconstituted into proteoliposomes, we investigated interaction with sterols, and effect various defined phospholipids sterols on basal ATP hydrolysis,...

The P‐glycoprotein multidrug transporter (Pgp, ABCB1) is an ABC protein that effluxes hundreds of structurally unrelated compounds from cells, and implicated in resistance many human cancers. membrane environment critically important for Pgp function since lipophilic substrates partition into, are pumped out of, the membrane. Using purified reconstituted into proteoliposomes defined lipids, effect bilayer melting transition on lipid coefficient, binding affinity, initial rate transport was...

Discussing works by Patrick Declerck, Ghassan Hage, and Giorgio Agamben, this paper asks whether society sets the homeless apart treats them way it does not accident, but deliberately, because they serve, as outcasts, a particular function. This article attempt to analyse given local or national situation; is, instead, thought experiment which develops hypothesis drawing on ideas put forward authors such Michel Foucault, Claude Lévi- Strauss Achille Mbembe. It posits that are result of an...