A5021799936- Enzyme Production and Characterization
- Fungal and yeast genetics research
- Glycosylation and Glycoproteins Research
- Protein Hydrolysis and Bioactive Peptides
- Enzyme Structure and Function
- Studies on Chitinases and Chitosanases
- Viral Infectious Diseases and Gene Expression in Insects
- Endoplasmic Reticulum Stress and Disease
- Heavy metals in environment
- Enzyme Catalysis and Immobilization
- Bacterial Genetics and Biotechnology
- Biopolymer Synthesis and Applications
- Pineapple and bromelain studies
- Amino Acid Enzymes and Metabolism
- CRISPR and Genetic Engineering
- Protease and Inhibitor Mechanisms
- Metabolism and Genetic Disorders
- Mycotoxins in Agriculture and Food
- Microbial Inactivation Methods
- Biotin and Related Studies
- Synthesis and Biological Evaluation
- Meat and Animal Product Quality
- Biochemical and Structural Characterization
- Mine drainage and remediation techniques
- Biofuel production and bioconversion
Guizhou Institute of Technology
2020-2024
South China Normal University
2021
Huaiyin Normal University
2012-2015
Jiangnan University
2013
A feather-degrading bacterium Pseudomonas aeruginosa C11 was isolated from feather dumping soil with quick plate screening technology and identified based on morphological biochemical characterization analysis of 16S rDNA sequences. The keratinolytic protease from P. purified 17.4-fold through ammonium sulphate precipitation, a sephadex G-75 gel i¬ltration column DEAE sepharose fast-i¬‚ow column. could deeply degrade raw feathers within 24 h at 37°C. relative molecular mass the estimated to...
Heavy metal pollution in soil has received much attention recent decades. Many studies have analyzed the interaction between specific quality and heavy pollution. However, there is little information about status, spatial distribution sources of metals province Tianjin. In this paper, characteristics were studied by means surface Tianjin, as study area object, conducted combination with land use types, using multiple data analysis multivariate statistics, while levels evaluated various...
Yeasts are widely used for the production of heterologous proteins. Improving expression such proteins is a top priority pharmaceutical and industrial applications. N-Glycosylation, common form protein modification in yeasts, facilitates proper folding secretion. Accordingly, our previous study revealed that attachment additional N-glycans to recombinant elastase by introducing an N-glycosylation sequon at suitable locations could stimulate its expression. Interestingly, Asn-Xaa-Thr...
Chitooligosaccharides (COSs) with relatively higher degrees of polymerization (DP) exhibited better biological properties than COSs lower DP. Here, we demonstrated a potential strategy to enhance the affinity, activity, and thermostability chitosanase Csn75 from Aspergillus fumigatus CJ22-326 through fusion carbohydrate binding module (CBM) that can specifically bind chitosan. Compared Csn75, specific enzyme activity Csn75-CBM32 Csn75-2CBM32 had increased by 59.18% 14.29%, respectively. The...
Pichia pastoris is one of the most popular eukaryotic hosts for producing heterologous proteins, while increasing secretion target proteins still a top priority their application in industrial fields. Recently, research effort to enhance protein production has focused on up-regulating unfolded response (UPR).We evaluated effects activated UPR via Hac1p co-expression with promoter AOX1 (PAOX1) or GAP (PGAP) expression recombinant chitosanase (rCBS) P. pastoris.The DNA sequence encoding was...
Abstract Objective The objective was to develop a convenient strategy for co-expressing multiple proteins in Komagataella phaffii via the Cre/ loxP system without introducing any markers. Results A plasmid this generated from pPICZαA with integration of lox71-Sh ble-lox66 . Firstly, inserted one target protein gene and then transformed into K. KM71. Secondly, auxiliary pPICZαA/cre/his4 containing CRE recombinase further chromosomally Sh ble therein. Finally, methanol induction conducted...