A5103005254- HIV Research and Treatment
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- HIV/AIDS drug development and treatment
- vaccines and immunoinformatics approaches
- HIV/AIDS Research and Interventions
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Plant Micronutrient Interactions and Effects
- Agriculture, Soil, Plant Science
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- Fibroblast Growth Factor Research
- Cytomegalovirus and herpesvirus research
- Plant nutrient uptake and metabolism
- Soil Carbon and Nitrogen Dynamics
- Diabetes Treatment and Management
- RNA Research and Splicing
- Food Quality and Safety Studies
- Allelopathy and phytotoxic interactions
- Corrosion Behavior and Inhibition
- Cell Adhesion Molecules Research
- Cardiovascular Function and Risk Factors
- Heavy metals in environment
- Electrodeposition and Electroless Coatings
Xinxiang Medical University
2025
Guizhou University
2023-2024
Affiliated Hospital of Jining Medical University
2019-2024
Guizhou Normal University
2024
Jiujiang First People's Hospital
2024
Bridge University
2024
Honghe University
2024
Second Affiliated Hospital of Nanchang University
2013-2023
Nanchang University
2013-2023
Southeast University
2022-2023
FoundationOne®CDx (F1CDx) is a United States (US) Food and Drug Administration (FDA)-approved companion diagnostic test to identify patients who may benefit from treatment in accordance with the approved therapeutic product labeling for 28 drug therapies. F1CDx utilizes next-generation sequencing (NGS)-based comprehensive genomic profiling (CGP) technology examine 324 cancer genes solid tumors. reports known likely pathogenic short variants (SVs), copy number alterations (CNAs), select...
Osteoarthritis (OA) is the most prevalent joint disease, yet effective disease-modifying OA drugs (DMOADs) remain elusive. Targeting macrophage polarization has emerged as a promising avenue for treatment. This study identified skatole through high-throughput screening an efficient modulator of polarization. In vivo experiments demonstrated that administration markedly reduced synovitis and cartilage damage in both destabilization medial meniscus (DMM)-induced mice monosodium iodoacetate...
Mutations within cytotoxic T lymphocyte (CTL) epitopes impair cell recognition, but escape mutations arising in flanking regions that alter antigen processing have not been defined natural human infections. In histocompatibility leukocyte (HLA)-B57+ HIV-infected persons, immune selection pressure leads to a mutation from alanine proline at Gag residue 146 immediately preceding the NH2 terminus of dominant HLA-B57–restricted epitope, ISPRTLNAW. Although N-extended wild-type or mutant peptides...
Cytotoxic T lymphocytes (CTLs) play a vital part in controlling viral replication during human infections. Most studies infections have focused on CTL specificities chronic infection and few data exist regarding the specificity of initial response induced acute infection. In this study, HIV-1 persons expressing histocompatibility leukocyte antigen (HLA)-A*0201 was used as means addressing issue. infection, dominant HLA-A*0201-restricted is directed towards epitope SLYNTVATL ("SL9") p17 Gag...
The emergence of cytotoxic T-lymphocyte (CTL) escape mutations in human immunodeficiency virus type 1 (HIV-1) proteins has been anecdotally associated with progression to AIDS, but it difficult determine whether viral mutation is the cause or result increased replication. Here we describe a perinatally HIV-infected child who maintained plasma load <400 copies/ml for almost decade until nonbinding emerged within immunodominant CTL epitope. subsequently experienced reemergence HIV-1 viremia...
CD8 T-cell responses are thought to be crucial for control of viremia in human immunodeficiency virus (HIV) infection but ultimately fail most infected persons. Studies acute have demonstrated strong CD8-mediated selection pressure and evolution mutations conferring escape from recognition, the ability that persist late-stage recognize viruses present vivo has not been determined. Therefore, we studied 24 subjects with advanced HIV disease (median viral load = 142,000 copies/ml; median CD4...
The highly sensitive quantitation of virus-specific CD8(+) T cells using major histocompatibility complex-peptide tetramer assays has revealed higher levels cytotoxic lymphocytes (CTLs) in acute and chronic virus infections than were recognized previously. However, studies lymphocytic choriomeningitis infection have shown that may include measurement a substantial number tetramer-binding are functionally inert. Such phenotypically silent CTLs, which lack cytolytic function do not produce...
ABSTRACT Cytotoxic T-lymphocyte (CTL) activity plays a central role in control of viral replication and determining outcome cases human immunodeficiency virus type 1 (HIV-1) infection. Incorporation important CTL epitope sequences into candidate vaccines is, therefore, vital. Most studies have focused upon small numbers adult Caucasoid subjects infected with clade-B virus, whereas the global epidemic is most severe sub-Saharan African populations predominantly involves clade-C infection both...
Abstract Mutational escape from the CTL response represents a major driving force for viral diversification in HIV-1-infected adults, but during infancy has not been described previously. We studied immune of perinatally infected children to an epitope (B57-TW10) that is targeted early acute HIV-1 infection adults expressing HLA-B57 and rapidly mutates under this selection pressure. Viral sequencing revealed universal presence mutations within TW10 among B57- B5801-positive children....
The relative contributions of HLA alleles and T-cell receptors (TCRs) to the prevention mutational viral escape are unclear. Here, we examined human immunodeficiency virus type 1 (HIV-1)-specific CD8(+) responses restricted by two closely related class I alleles, B*5701 B*5703, that differ amino acids but both associated with a dominant response same HIV-1 Gag epitope KF11 (KAFSPEVIPMF). When this is presented HLA-B*5701, it induces TCR repertoire highly conserved among individuals,...
Abstract Hypertension is a significant risk factor of cardiovascular diseases (CVDs) with high prevalence worldwide, the current treatment has multiple adverse effects and requires continuous administration. The glucagon‐like peptide‐1 receptor (GLP‐1R) agonists have shown great potential in treating diabetes mellitus, neurodegenerative diseases, obesity hypertension. Butyric acid target Yet, application GLP‐1 analogue butyric reducing blood pressure reversing ventricular hypertrophy remains...
ABSTRACT Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL) play a major role in control of viral replication. To understand the contribution this antiviral response, an initial step is to fully define specific epitopes targeted by CTL. These studies focused on CTL responses restricted HLA-A∗3002, one HLA-A molecules most prominent African populations. avoid time-consuming effort and expense involved culturing prior defining restricting alleles, we developed method...
Abstract High fibroblast growth factor 23 (FGF23) concentrations are a strong predictor of atrial fibrillation (AF), but researchers have not clearly determined the mechanism by which FGF23 causes fibrosis in patients with AF. This study aims to elucidate induces Immunohistochemistry was used expression FGF23, FGFR4, and fibrotic factors normal sinus rhythm (SR) Cardiac fibroblasts (CFs) were cocultured different recombinant protein. Compared SR group, levels α‐smooth muscle actin (α‐SMA),...
Abstract Perinatal HIV infection is characterized by a sustained high-level viremia and high risk of rapid progression to AIDS, indicating failure immunologic containment the virus. We hypothesized that age-related differences in specificity or function HIV-specific T cells may influence RNA levels clinical outcome following perinatal infection. In this study, we defined epitopes targeted 76 pediatric subjects (47 infected 29 exposed, but uninfected), assessed ability CD8 CD4 degranulate...
ABSTRACT Cytotoxic-T-lymphocyte (CTL) escape mutations undermine the durability of effective human immunodeficiency virus type 1 (HIV-1)-specific CD8 + T cell responses. The rate CTL from a given response is largely governed by net all escape-associated viral fitness costs and benefits. observation that can carry an associated cost in terms reduced replication capacity (RC) suggests cost-benefit trade-off could delay thereby prolong effectiveness. However, our understanding this potential...
ABSTRACT Expression of HLA-B57 is associated with restricted replication human immunodeficiency virus (HIV), but the mechanism for its protective effect remains unknown. If this advantage depends upon CD8 T-cell recognition B57-restricted epitopes, mother-to-child transmission escape mutations within these epitopes could nullify effect. However, if B57 largely mediated by selection fitness-attenuating viral such as T242N in TW10-Gag, then facilitate control haploidentical infant. We assessed...
Aging is regarded as a dominant risk factor for cancer. Additionally, inflammation and asthenic immune surveillance with aging may facilitate tumor formation development. However, few studies have comprehensively analyzed the relationship between aging-related genes (AGs) prognosis, immunity of head neck squamous cell carcinoma (HNSCC). Here, we initially screened 41 differentially expressed AGs from The Cancer Genome Atlas (TCGA) database. In training set, prognosis model seven (APP,...
ABSTRACT Viral peptides are recognized by cytotoxic T lymphocytes (CTL) as a complex with major histocompatibility (MHC) class I molecules, but the extent to which single HLA allele can accommodate epitope of different length and sequence is not well characterized. Here we report identification clonal CTL responses from same donor that independently recognize one two HLA-B57-restricted epitopes, KAFSPEVIPMF (KF11; p24 Gag residues 30 40) KAFSPEVI (KF8; 37). Although lysis studies indicated...
MicroRNAs, a group of noncoding regulatory RNAs, are involved in oncogenesis, cell survival, and chemosensitivity. First, microarray-based analysis predicted that far-upstream element-binding protein 2 (FBP2) was upregulated hepatocellular carcinoma (HCC), which may be regulated by miR-591. In this study, we hypothesize an inhibitory role miR-591 HCC via regulating FBP2. Next, reverse transcription quantitative polymerase chain reaction found FBP2 expressed highly expresses poorly tissues....
Abstract Background Carbapenem-resistant E. coli (CREco) pose a significant public health threat due to their multidrug resistance. Colistin is often last-resort treatment against CREco; however, the emergence of colistin resistance gene mcr-1 complicates options. Methods Two strains (ECO20 and ECO21), recovered from hospitalized patients in distinct wards, exhibited carbapenems colistin. Whole-genome sequencing phenotypic characterization were employed study patterns, plasmid profiles,...