A5057402100- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Receptor Mechanisms and Signaling
- Musculoskeletal pain and rehabilitation
- Neurobiology and Insect Physiology Research
- Anesthesia and Pain Management
- Pain Management and Placebo Effect
- Ion Channels and Receptors
- Gastrointestinal motility and disorders
- Pain Management and Opioid Use
- Neuroendocrine regulation and behavior
- Transcranial Magnetic Stimulation Studies
- Veterinary Pharmacology and Anesthesia
- Hormonal and reproductive studies
- Sleep and Wakefulness Research
- Botulinum Toxin and Related Neurological Disorders
- Pharmacological Effects of Natural Compounds
- Pediatric Pain Management Techniques
- Complementary and Alternative Medicine Studies
- Pharmacology and Obesity Treatment
- Neuroscience of respiration and sleep
- Listeria monocytogenes in Food Safety
- Sports Performance and Training
U.S. Army Engineer Research and Development Center
2020-2023
Brewer Science (United States)
2023
Missouri State University
2023
Eli Lilly (United Kingdom)
2021
AstraZeneca (United States)
2017
AstraZeneca (Canada)
2003-2013
McGill University
2004-2013
Eli Lilly (United States)
2013
AstraZeneca (Brazil)
2004-2012
McGill University Health Centre
2012
The generation of transgenic mice that lack or overexpress genes relevant to pain is becoming increasing common. However, only one visceral model, the writhing test, widely used in mice. Here we describe a novel chemical stimulation colon, which have developed Mice either sex were injected i.v. with 30 mg/kg Evan's Blue for subsequent determination plasma extravasation. For behavioural testing, they placed on raised grid and 50 μl saline, mustard oil (0.25–2.5%) capsaicin (0.03–0.3%) was...
1. Extracellular single-unit recordings have been made from 295 dorsal horn neurons in the lumbar enlargement of rat spinal cord; 191 20 rats with an experimental peripheral neuropathy, and 104 10 sham-operated rats. Recordings were 9-11 days after inducing neuropathy by tying four loose ligatures around sciatic nerve nerve-injured or performing a sham procedure 2. A survey general properties all encountered was (104 neurons) compared those seen 17 animals (180 neurons). The vast majority...
The tetrodotoxin-resistant sodium channel alpha subunit Nav1.8 is expressed exclusively in primary sensory neurons and proposed to play an important role sensitization of nociceptors. Here we compared visceral pain referred hyperalgesia Nav1.8-null mice their wild-type littermates five tests that differ the degree which behavior depends on spontaneous, ongoing firing sensitized showed normal nociceptive provoked by acute noxious stimulation abdominal viscera (intracolonic saline or...
The tetrodotoxin‐resistant sodium channel α subunit, Nav1.8, is exclusively expressed in primary sensory neurons and suggested to play a role the generation of ectopic action potentials after axonal injury thereby contribute neuropathic pain. Here we investigated involvement Nav1.8 impulse damaged axons by examining spontaneous activity mechanosensitivity neuromas formed section saphenous nerve null mice their wild‐type littermates. We recorded 522 identified units from 24 vitro at two time...
The persistent increase in pain sensitivity observed after injury, known as hyperalgesia, depends on synaptic plasticity the pathway, particularly spinal cord. Several potential mechanisms have been proposed, including post-synaptic exocytosis of AMPA subclass glutamate receptors (AMPA-R), which is to play a critical role hippocampus. AMPA-R trafficking has described neurons culture but it unknown if can also occur vivo, or be induced by natural painful stimulation. Here we referred...
Cannabinoids are analgesic in man, but their use is limited by psychoactive properties. One way to avoid cannabinoid receptor subtype 1 (CB1R)-mediated central side-effects develop CB1R agonists with CNS penetration. Activation of peripheral CB1Rs has been proposed be analgesic, the relative contribution effects systemic cannabinoids remains unclear. Here we addressed this exploring properties and site action AZ11713908, a peripherally restricted agonist, rodent pain models. Systemic...
1. Single-unit electrical activity has been recorded from 42 dorsal horn neurons in the sacral segments of rat's spinal cord. The sample consisted 20 multireceptive (class 2) cells with both A- and C-fiber inputs 22 nocireceptive 3) cells. All had cutaneous receptive fields (RFs) on tail. 2. RF sizes their response thresholds to mechanical stimulation skin were determined before after each a series 2-min noxious stimuli. Up five such stimuli delivered at intervals ranging 10 60 min. In most...
The sensory neuron-specific G protein coupled receptors (SNSRs) have been described as a family of whose expression in small diameter neurons the trigeminal and dorsal root ganglia suggests an implication nociception. To date, physiological function(s) SNSRs remain unknown. Hence, aim present study was to determine effects rat SNSR1 activation on nociception rats. pharmacological characterization initially performed vitro identify specific ligand, which could be used subsequently for...
N-methyl-D-aspartate (NMDA) receptors appear to play little part in nociceptive responses evoked by acute stimulation of normal somatic tissues, but rather are involved hyperalgesic after peripheral injury and inflammation. Previous studies from this laboratory have shown important differences the neural organization visceral pathways. Here, we explored role NMDA processing noxious input, compared with input. The left ureter was cannulated close bladder adult female Wistar rats anaesthetized...
The generation of transgenic ‘knock‐out’ mice which lack genes relevant to pain is becoming increasing common. However, only one visceral model, the writhing test, available in mice. aim this study was adapt cyclophosphamide cystitis, a model inflammatory described rats, for use mice, and characterise its behavioural effects. toxic metabolites systemically‐administered are excreted urine, induce bladder inflammation. We compared effects (100 300 mg/kg i.p., 4 h survival period) vehicle...
1. In order to examine the contribution of neurokinin1 (NK1) receptors facilitation a spinal nociceptive reflex in rat, we have investigated effects RP 67580 (3aR, 7aR)-7,7-diphenyl-2(1-imino-2-(2-methoxyphenyl/ethyl)perhydrois oindole)), non-peptide receptor antagonist, selective for rodent sub-type, on activity individual motorunits. These results were compared with 68651, inactive 3aS, 7aS enantiomer 67580, as control non-specific activity. 2. Experiments performed 15 rats anaesthetized...
1. Single‐unit electrical activity has been recorded from dorsal horn neurones in the sacral (S1‐S2) segments of spinal cord barbiturate‐anaesthetized rats. Fifty‐two responding to a manually applied pinch their receptive fields tail were selected. They subsequently tested for responses four successive 2 min pinches at noxious intensities delivered by feed‐back‐controlled mechanical device. 2. Neurones with both innocuous (i.e. brushing and stroking) pinching, pin‐prick, some cases heating...
This study was designed to examine the central changes in receptive field properties of dorsal horn neurones induced by a period visceral noxious stimulation. The aim this investigation establish whether stimulation input spinal cord could influence transmission cutaneous information through neurones. Single-unit electrical activity recorded lower thoracic anaesthetized cats from with somatic ipsilateral flank. Changes these fields reversible spinalization (by means cold block 4 or 5...
Roza, Carolina, Jennifer M. A. Laird, and Fernando Cervero. Spinal mechanisms underlying persistent pain referred hyperalgesia in rats with an experimental ureteric stone. J. Neurophysiol. 79: 1603–1612, 1998. neurons processing information from the ureter have been characterized 1–4 days after implantation of stone compared those normal rats. The effects a conditioning noxious stimulation presence evoked by calculosis also were examined. Extracellular recordings performed at T 12 –L 1...