A5007469757- Reproductive Biology and Fertility
- Analytical chemistry methods development
- Sperm and Testicular Function
- Ovarian function and disorders
- Heavy metals in environment
- Birth, Development, and Health
- Reproductive Physiology in Livestock
- Electrochemical Analysis and Applications
- Analytical Chemistry and Chromatography
- Mercury impact and mitigation studies
- Renal and related cancers
- Advanced Chemical Sensor Technologies
- Saffron Plant Research Studies
- Circadian rhythm and melatonin
- X-ray Spectroscopy and Fluorescence Analysis
- Radioactive element chemistry and processing
- Analytical Methods in Pharmaceuticals
- Ion-surface interactions and analysis
- Chronic Disease Management Strategies
- Electrostatics and Colloid Interactions
- Coal and Its By-products
- Radioactive contamination and transfer
- Genomics and Rare Diseases
- Mass Spectrometry Techniques and Applications
- Radioactivity and Radon Measurements
University of Oxford
2023
Universitat Autònoma de Barcelona
2010-2022
Hospital Sant Joan de Déu Barcelona
2020
Institut de Recerca Sant Joan de Déu
2020
National University of San Luis
2002
Universitat de Barcelona
1985-1997
Universitat de València
1989
Universitat de les Illes Balears
1988
The aim of this study was to test the Brilliant Cresyl Blue (BCB) stain select prepubertal sheep oocytes for in vitro blastocyst production. Oocyte diameter, mitochondrial activity, maturation-promoting factor (MPF) activity and mRNA relative expression (RE) genes related metabolism (ATPase Na(+)/K(+) transporting α 1 (ATP1A1) cytochrome c oxidase subunit (COX1)) constitutive function cell (cytoplasmic polyadenylation-element-binding protein (CPEB) S100A10) were assessed. Immature exposed...
Melatonin is a universal antioxidant that improves in vitro embryo production several species. The aims of this study were to determine the melatonin concentration ovarian follicular fluid (FF) juvenile goats and effect during maturation (IVM) on development. FF was 0.57--1.07×10-9 M, increasing with diameter. Oocytes matured, fertilised cultured under conventional conditions. Blastocyst development, quality levels reactive oxygen species (ROS) reduced glutathione assessed. In Experiment 1...
Melatonin enhances in vitro embryo development several species by improving the oocyte developmental competence during maturation (IVM). has a wide range of actions, from scavenging reactive oxygen (ROS) to regulating gene expression, and it can also act way melatonin receptors. The aim this study was determine mechanism action IVM juvenile goat oocytes role membrane receptor 1 immunolocalized cumulus cells before after 24 hr IVM. effect on tested three experimental groups: (a) control, (b)...
Contents The aim of this study was to test the effect insulin–transferrin–selenium (ITS) and L‐ascorbic acid (AA) supplementation hormonal level during in vitro maturation (IVM) small oocytes from pre‐pubertal goat on blastocyst yield quality. Concretely, we used four media: conventional IVM medium (CM), growth (GM: CM+ITS+AA low hormones), modified CM (mCM: with hormones) GM (mGM: normal hormones). Cumulus–oocyte complexes (COCs) were classified into two categories according oocyte...
In goats, embryo oocyte competence is affected by follicle size regardless the age of females. previous studies we have found differences in blastocyst development between oocytes coming small (<3 mm) and large follicles (>3 prepubertal (1−2 months-old) goats. Oocyte Follicular Fluid (FF) composition changes throughout growth. The aim this study was to analyze Fatty Acids (FAs) metabolomic profiles FF recovered from goats adult FAs were analyzed chromatography metabolites 1H-Nuclear Magnetic...
Methods for the direct determination of Cd and Mo in soil extracts by graphite furnace atomic-absorption spectrometry (GFAAS) inductively coupled plasma (ICP) were developed optimum operating conditions, analyte modifiers matrix interferences investigated. The use a mini-furnace allowing an atomisation temperature 1200 °C addition + H2O2+ HNO3 as modifier are most important features successful GFAAS. characteristic amounts 0.164 pg 8.39 GFAAS, with relative standard deviations (RSD) 7.5% 4%,...
In this study we assessed the concentration of linoleic acid (LA) and linolenic (ALA) in follicular fluid prepubertal goats according to follicle size (<3mm or ≥3mm) by gas chromatography tested addition different LA ALA (LA:ALA) ratios (50:50, 100:50 200:50µM) IVM medium on embryo development, mitochondrial activity, ATP relative gene expression (RPL19, ribosomal protein L19; SLC2A1, facilitated glucose transporter 1; ATF4, activating transcription factor 4; GPX1, glutathione peroxidase...
A method for the determination of trace amounts Pd, Fe and Cu in pure gold has been established using inductively coupled plasma atomic emission spectrometry (ICP-AES). The most suitable spectral lines analysis have selected by considering two essential criteria: sensitivity interferences. samples were dissolved with aqua regia determinations carried out direct calibration synthetic standards containing regia. Precision detection limits investigated. inhomogeneity sample was inferred from a...
In our laboratory, we have been working for more than 20 years on in vitro embryo production from prepubertal goat oocytes. We observed significant differences blastocyst according to the season of year. The present study is aimed asses these summer, autumn, winter, and spring. Oocytes with 3 compact cumulus layers (cumulus-oocyte complex, COC) homogeneous cytoplasm were obtained after slicing ovaries (45-day-old Murciano-Granadina rearing animals) recovered slaughterhouse. Groups 30 COC...
The aim of this study was to evaluate the utility brilliant cresyl blue (BCB) test as an indirect measure oocyte growth select competent prepubertal sheep oocytes for in vitro embryo production. BCB stain allows determination glucose–6-phosphate dehydrogenase (G6PD) activity, enzyme with decreased activity that have finished their phase. Oocytes were obtained after slicing surface lamb ovaries (2–5 months old) from a local abattoir. more than 3 compact cumulus layers and homogeny cytoplasm...
Abstract A method for the direct determination of Cd in soil extracts by graphite furnace atomic absorption spectrosopy (GFAAS) has been developed. Optimum operating conditions, analyte modifiers and matrix interferences have investigated. Characteristic amounts are 0.34pg siliceous samples 0.45 pg calcareous soils. The precision based on ten replicate readings standards, was 1.6%. determined proposed methods results compared with those obtained ICP technique.
Brilliant cresyl blue (BCB) staining determines the activity of glucose-6-phosphate dehydrogenase (G6PDH), an enzyme in which decreases as oocytes reach their growth phase. We have previously shown lamb effectiveness this stain selecting largest and most competent to develop up blastocyst stage (Catala et al. 2011 Reproduction 142, 517–527). The aim study was analyse expression 4 genes related oocyte quality BCB-selected oocytes. analyzed were selected after a literature review they...