Cannabidiol (CBD), the major non-psychotomimetic compound present in Cannabis sativa plant, exhibits therapeutic potential for various human diseases, including chronic neurodegenerative such as Alzheimer's and Parkinson's, ischemic stroke, epilepsy other convulsive syndromes, neuropsychiatric disorders, neuropathic allodynia certain types of cancer. CBD does not bind directly to endocannabinoid receptors 1 2, despite research efforts, its specific targets remain be fully identified....

Overactivation of glutamate N-methyl-D-aspartate receptor (NMDAR) increases the cytosolic concentrations calcium and zinc, which significantly contributes to neural death. Since cannabinoids prevent NMDAR-mediated increase in calcium, we investigated whether they also control rise potentially toxic free zinc ions, as well processes implicated this phenomenon.The cannabinoid receptors type 1 (CNR1) NMDARs are cross-regulated different regions nervous system. Cannabinoids abrogated stimulation...

The in vivo pharmacology of the sigma 1 receptor (σ1R) is certainly complex; however, σ1R antagonists are therapeutic interest, because they enhance mu-opioid (MOR)-mediated antinociception and reduce neuropathic pain. Thus, we investigated whether involved negative control that glutamate N-methyl-d-aspartate acid receptors (NMDARs) exert on opioid antinociception.The MOR C terminus carries histidine triad nucleotide-binding protein (HINT1) coupled to regulator G-protein signaling...

// María Rodríguez-Muñoz 1 , Elsa Cortés-Montero Andrea Pozo-Rodrigálvarez Pilar Sánchez-Blázquez and Javier Garzón-Niño Department of Molecular, Cellular Developmental Neurobiology, Laboratory Neuropharmacology. Instituto Cajal, Consejo Superior de Investigaciones Científicas (CSIC). Madrid, Spain Correspondence to: Garzón-Niño, email: Keywords : σ1R, HINT1 protein, cannabinoid CB1 receptor, neurological disorders, mu-opioid Pathology Section Received June 30, 2015 Accepted September 23,...

Defatted grape seed meal (DGSM) is a residue obtained from pomace and an important source of protein. The aim this study was to select peptides with optimal antioxidant colour properties, using enzymes different origins proteolytic character, for application in winemaking. For purpose, the assay performed novo-ProD (NP), alcalase (AL), novozym (NZ), pepsin (PE), flavourzyme (FZ), papain (PA) enzymes. peptide percentage, yield, molecular size fractions, total amino acid, content, activity,...

In mouse periaqueductal gray matter (PAG) membranes, the mu-opioid receptor (MOR) coprecipitated alpha-subunits of Gi/o/z/q/11 proteins, Gbeta1/2 subunits, and regulator G-protein signaling RGS9-2 its partner protein Gbeta5. RGS7 RGS11 present in this neural structure showed no association with MOR. vivo intracerebroventricular injection morphine did not alter MOR immunoreactivity, but 30 min 3 h after administration, coprecipitation Galpha subunits MORs was reduced by up to 50%....

Background In periaqueductal grey (PAG) matter, cross-talk between the Mu-opioid receptor (MOR) and glutamate N-methyl-D-Aspartate (NMDAR)-CaMKII pathway supports development of analgesic tolerance to morphine. neurons, histidine triad nucleotide binding protein 1 (HINT1) connects regulators G signaling RGSZ1 RGSZ2 C terminus MOR. response morphine, this HINT1-RGSZ complex binds PKCγ, afterwards, interplay Src Gz/Gi proteins leads sustained potentiation NMDAR-mediated responses....

Through the cannabinoid receptor 1 (CB1), endocannabinoid system plays a physiological role in maintaining activity of glutamate N-methyl-D-aspartate (NMDA) within harmless limits. The influence cannabinoids must be proportional to stimulus order prevent NMDAR overactivation or exaggerated hypofunction that may precipitate symptoms psychosis. In this framework, recently reported association CB1s with NMDARs, which mediates reduction analgesia promoted by antagonism, could also support...

Abstract Background G protein-coupled receptors (GPCRs) are the targets of a large number drugs currently in therapeutic use. Likewise, glutamate ionotropic N -methyl-D-aspartate receptor (NMDAR) has been implicated certain neurological disorders, such as neurodegeration, neuropathic pain and mood well psychosis schizophrenia. Thus, there is now an important need to characterize interactions between GPCRs NMDARs. Indeed, these can produce distinct effects, whereas activation Mu-opioid (MOR)...

Abstract The activity of certain G protein-coupled receptors (GPCRs) and glutamate N -Methyl-D-aspartate (NMDARs) is altered in both schizophrenia depression. Using postmortem prefrontal cortex samples from subjects with or depression, we observed a series opposite changes the expression signaling proteins that have been implicated cross-talk between GPCRs NMDARs. Thus, levels HINT1 NMDAR NR1 subunits carrying C1 cytosolic segment were increased depressives decreased schizophrenics, respect...

// María Rodríguez-Muñoz 1 , Pilar Sánchez-Blázquez and Javier Garzón Neuropharmacology, Department of Translational Neurosciences, Cajal Institute, CSIC, Madrid E-28002, Spain Correspondence to: Garzón, email: jgarzon@cajal.csic.es Keywords: fenfluramine; seizures; glutamate N-methyl-D-aspartate receptor; type sigma HINT1 protein Received: January 04, 2018      Accepted: April 03, Published: May 2018 ABSTRACT...

Background Although the systemic administration of cannabinoids produces antinociception, their chronic use leads to analgesic tolerance as well cross-tolerance morphine. These effects are mediated by binding peripheral, spinal and supraspinal CB1 CB2 receptors, making it difficult determine relevance each receptor type these phenomena. However, in brain, receptors (CB1Rs) expressed at high levels neurons, whereas expression CB2Rs is marginal. Thus, CB1Rs mediate smoked cannabis also...