A5076906209- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Ovarian function and disorders
- MicroRNA in disease regulation
- Renal and related cancers
- Neuroscience and Neuropharmacology Research
- Cardiovascular Function and Risk Factors
- Magnetic and Electromagnetic Effects
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Animal Genetics and Reproduction
- Hormonal Regulation and Hypertension
- Circular RNAs in diseases
- Genomics and Chromatin Dynamics
- Proteins in Food Systems
- Lipid metabolism and biosynthesis
- TGF-β signaling in diseases
- Neuroendocrine regulation and behavior
- Metabolism, Diabetes, and Cancer
- Antimicrobial Peptides and Activities
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Adenosine and Purinergic Signaling
University of Turku
2011-2024
Uppsala University
2023
University of California, Berkeley
2016-2021
University of Helsinki
2009
The postnatal development of the epididymis is a complex process that results in highly differentiated epithelium, divided into several segments. Recent studies indicate role for RNA interference (RNAi) epididymis, however, actual requirement RNAi has remained elusive. Here, we present first evidence direct need differentiation epididymal epithelium.By utilizing Cre-LoxP system have generated conditional knock-out Dicer1 two most proximal segments mouse epididymis. Recombination Dicer1,...
Therapies that could further prevent the development of heart failure (HF) and other cardiovascular metabolic events in patients with recent myocardial infarction (MI) represent a large unmet medical need. DAPA-MI is multicenter, parallel-group, registry-based, randomized, double-blind, placebo-controlled phase 3 trial without known diabetes or established HF, presenting MI impaired left ventricular systolic function Q-wave MI. The evaluated effect dapagliflozin 10 mg vs placebo, given once...
During epididymal sperm maturation, the lipid content of membrane is modified, which facilitates motility and fertility. However, little known about mechanisms regulating maturation process. By generating a conditional knockout (cKO) Dicer1 in proximal part mouse epididymis, we studied role RNA interference functions. The cKO epididymis displayed an altered homeostasis associated with 0.6-fold reduction expression gene elongation very long chain fatty acids-like 2, enzyme needed for...
Maturation of bone morphogenetic proteins (BMPs) requires cleavage their precursor by furin-type proprotein convertases. Here, we find that sites the BMP2/4/decapentaplegic (DPP) subfamily have been evolutionary diversified and can be categorized into 4 different types. Cnidaria BMP2/4/DPP is considered to a prototype containing only 1 furin site. Bilateria acquired an additional site with either combination minimal-optimal or optimal-optimal sites. DPPs belonging Diptera, such as Drosophila...
Mammalian sperm gain their ability to fertilize the egg during transit through epididymis and by interacting with proteins secreted epididymal epithelial cells. Certain members of CRISP (cysteine-rich secretory protein) family form major protein constituent luminal fluid in mammalian epididymis. CRISP4 is newest member expressed predominantly Its structure expression pattern suggest a role maturation and/or sperm-egg interaction. To study relevance reproduction, we have generated Crisp4 iCre...
Dicer1 is an endoribonuclease involved in the biogenesis of functional molecules such as microRNAs (miRNAs) and endogenous small interfering RNAs (endo-siRNAs). These non-coding are important regulators post-transcriptional gene expression participate control male fertility. With knowledge that 1) Dicer1-dependent factors required for proper sperm maturation epididymis, 2) miRNAs potent mediators intercellular communication most biological systems, we investigated role produced by proximal...
Mammalian female fertility is defined by a successful and strictly periodic ovarian cycle, which under the control of gonadotropins steroid hormones, particularly progesterone estrogen. The latter two are produced ovaries that engaged in controlled follicular growth, maturation, release eggs, i.e., ovulation. hormones regulate cycles via genomic signaling, altering gene transcription protein synthesis. However, despite this well-studied mechanism, can also signal direct, non-genomic action,...
Abstract Background Spermatozoa leaving the testis are not able to fertilize egg in vivo . They must undergo further maturation epididymis. Proteins secreted epididymal lumen by epithelial cells interact with spermatozoa and enable these maturational changes, responsible for proper storage conditions before ejaculation. The present study was carried out order characterize expression of a novel Pate ( p rostate nd t estis e xpression) gene family, coding cysteine-rich proteins, Methods Murine...
Abstract Apart from the androgen receptor, transcription factors (TFs) that are required for development and formation of different segments epididymis have remained unknown. We identified TF families expressed in developing epididymides, which many showed segment specificity. From these TFs, down-regulation runt related (RUNXs) 1 2 expression coincides with epithelial regression Dicer1 cKO mice. Concomitant deletion both Runx1 Runx2 a mouse epididymal cell line affected morphology, adhesion...
The choroid plexus (CP) epithelium secretes cerebrospinal fluid and plays an important role in healthy homeostasis of the brain. CP function can be influenced by sex steroid hormones; however, precise molecular mechanism such regulation is not well understood. Here, using whole-cell patch-clamp recordings from male female murine cells, we show that application progesterone resulted specific strong potentiation inwardly rectifying potassium channel Kir7.1, essential protein expressed required...
Bmyc is a member of the Myc family transcriptional regulators in mouse and rat. It predominantly expressed hormonally controlled tissues, with highest level expression epididymis. The BMYC protein has been shown to function as transcription factor vitro inhibit MYC. To study significance vivo, knockout (KO) model was generated by homologous recombination. KO mice were viable fertile did not display gross morphological or histological changes compared WT mice. However, testes epididymides...
Key physiological functions of organisms, such as sensory transduction, regulation heart rate, smooth muscle contraction, bile secretion, endocrine regulation, immune responses, and various pathophysiological conditions, including neuropathic pain, diabetes, kidney failure, cancer, are
Abstract Therian female fertility is defined by a successful and strictly periodic ovarian cycle, which under the control of gonadotropins steroid hormones, particularly progesterone estrogen. The latter two are produced ovaries that engaged in controlled follicular growth, maturation release eggs, i.e. ovulation. It well known hormones regulate cycles via genomic signaling, altering gene transcription protein synthesis. However, despite this well-studied mechanism, can also signal direct,...