A5077621044- Plant Virus Research Studies
- Phytoplasmas and Hemiptera pathogens
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Forest Insect Ecology and Management
- Cocoa and Sweet Potato Agronomy
- Insect-Plant Interactions and Control
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Oil Palm Production and Sustainability
- Research on scale insects
- Agriculture and Rural Development Research
- Berry genetics and cultivation research
- Plant tissue culture and regeneration
- Biological Control of Invasive Species
- Insect and Pesticide Research
- Plant Reproductive Biology
- Date Palm Research Studies
- Agricultural Practices and Plant Genetics
- Plant Physiology and Cultivation Studies
- Plant-Microbe Interactions and Immunity
- Insect symbiosis and bacterial influences
- Toxin Mechanisms and Immunotoxins
- Horticultural and Viticultural Research
- Plant Disease Management Techniques
Walloon Agricultural Research Centre
2011-2024
In 2014, Physostegia chlorotic mottle virus (PhCMoV) was discovered in Austria
In this study, an extensive virome investigation was performed on a germplasm collection of pear trees (Pyrus communis L.) from the Walloon Agricultural Research Centre (Gembloux, Belgium). total,128 samples were analyzed as pools using high-throughput sequencing (HTS) techniques, and/or tested individually for targeted viruses by RT-PCR. During survey, novel velarivirus identified in several asymptomatic while four known detected. Bioinformatics tools used to assemble genome new virus. The...
Recent identifications of Chrysanthemum stunt viroid (CSVd) and Potato spindle tuber (PSTVd) in Solanum jasminoides (3,4) prompted the testing this plant species for infections with other pospiviroids. From autumn 2006 to spring 2007, samples from symptomless plants S. were collected Belgium (3 ranging 75 150 plants), Germany 1 200 Netherlands 2 plants). Samples tested pospiviroids by reverse transcription (RT)-PCR assays using Pospi1-FW/RE Vid-FW/RE (2) PSTV-Nb-FW...
In order to assess the occurrence of Wheat spindle streak mosaic virus (WSSMV) in Belgium, a reverse-transcription polymerase chain reaction (RT-PCR) was developed, targeting WSSMV isolates from Canada, France, Germany, Italy, and United States. The primers also were designed for quantification by real-time RT-PCR with SYBR-Green. No cross-reaction soilborne cereal viruses such as Barley mild virus, yellow Soilborne Soil-borne wheat observed. quantitative allowed more sensitive detection...
During August of 2006, a sample tomato plant (Solanum lycopersicum, formerly Lycopersicum esculentum) from greenhouse in Belgium was received for diagnosis. The showed severe growth reduction and the young leaves were chlorotic distorted. In greenhouse, disease had been spreading slowly along row. These observations suggested presence viroid infection, reverse transcriptase (RT)-PCR with two sets universal pospiviroid primers (Pospi1-RE/FW Vid-FW/RE; 3) yielded amplicons expected size...
In summer 2007, leaves and fruits from tomato (Solanum lycopersicum) cv. Santa with symptoms of virus-like disease were received for diagnosis glasshouse crops in Western France. Samples tested negative by ELISA common viral diseases: Cucumber mosaic virus, Tobacco Tomato spotted wilt viruses the Potyvirus group, Pepino virus yellow leaf curl virus. Affected plants displayed viroid-like including severe yellowing or reddening, addition to distortion stunting. To determine whether caused a...
Abstract Recent technological development of molecular methods has led to the proliferation new rapid PCR or reverse‐transcriptase (RT)‐PCR‐derived diagnostic tests for plant viruses. Nevertheless, routine use, reliability all these is not widely established and there still an apprehension adopt them in official certification material. This partly because lack confidence obtained results poor knowledge on reproducibility limits RT‐PCR protocols. There a information adequate risk assessment...
For a pilot study on germplasm collections, leaves were collected from six apple trees (Malus domestica) numbered Q9, Q27, Q35, Q37, Q39 and Q41 in Belgian experimental orchard (CRA-W) June 2019 2020. At the time of sampling during subsequent visits, no viral symptoms observed trees. However, Apple luteovirus 1 (ALV-1) has been identified with Rapid decline, rubodvirus (ARWV-1) is associated rubbery wood disease, hammerhead viroid (AHVd) infection presents variable including swelling or limb...
During a routine screening of ornamentals by the Federal Agency for Safety Food Chain (FASFC) in Belgium, pospiviroid was detected symptomless Brugmansia sp. (angel's trumpets) coming from Netherlands. Detection performed on leaf sample reverse transcription (RT)-PCR, first using universal primer pair (2) VIR1/VIR2 and subsequently with semispecific (3) CEVd-FW/RE, to amplify whole viroid genome. Amplicons expected sizes (260 360 bp, respectively) were sequencing CEVd-FW/RE amplicon (GenBank...
Abstract A Belgium‐wide survey to determine the presence of ‘ Candidatus Phytoplasma ulmi’ elm trees was performed on nearly 600 trees. The randomly sampled elms were screened for phytoplasmas using a standard generic nested PCR method. In five symptomless trees, detected. Multiple gene sequence analysis (16S rRNA, tuf, rplV‐rpsC‐rplP), virtual RFLP 16S rRNA fragment and phylogenetic showed that phytoplasma strains detected in all could be classified into 16SrXII‐E subgroup (‘...
Abstract The number of small-scale diversified vegetable growers in industrialized countries has risen sharply over the last ten years. risks associated with plant viruses these systems have been barely studied Europe, yet dramatic virus emergence events, such as tomato brown fruit rugose virus, sometimes occur. We developed a methodology that aimed to understand better implications related for production Belgian’s farms by comparing growers’ perception presence viral symptoms (visual...
The number of small-scale diversified vegetable growers in industrialized countries has risen sharply over the last 10 years. risks associated with plant viruses these systems have been barely studied Europe, yet dramatic virus emergence events, such as tomato brown fruit rugose (ToBRFV), sometimes occur. We developed a methodology that aimed to understand better implications related for production Belgian’s farms by comparing growers’ perception and presence plant-viral-like symptoms...