A5086134177- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Health, Environment, Cognitive Aging
- Lysosomal Storage Disorders Research
- Cellular Mechanics and Interactions
- Glycogen Storage Diseases and Myoclonus
- Biochemical and Molecular Research
- Molecular Biology Techniques and Applications
RTI International
2022-2024
Duke Medical Center
2017
Recent advances in fluorescence microscopy techniques and tissue clearing, labeling, staining provide unprecedented opportunities to investigate brain structure function. These experiments' images make it possible catalog cell types define their location, morphology, connectivity a native context, leading better understanding of normal development disease etiology. Consistent annotation metadata is needed the context necessary understand, reuse, integrate these data. This report describes an...
Selecting and implementing a tissue clearing protocol is challenging. Established more than 100 years ago, still rapidly evolving field of research. There are currently many published protocols to choose from, each performs better or worse across range key evaluation factors (e.g., speed, cost, stability, fluorescence quenching). Additionally, often optimized for specific experimental contexts, applying an existing new problem can require lengthy period adaptation by trial error. Although...
Recent advances in fluorescence microscopy techniques and tissue clearing, labeling, staining provide unprecedented opportunities to investigate brain structure function. These experiments' images make it possible catalog cell types define their location, morphology, connectivity a native context, leading better understanding of normal development disease etiology. Consistent annotation metadata is needed the context necessary understand, reuse, integrate these data. This report describes an...
ABSTRACT Selecting and implementing a tissue-clearing protocol is challenging. Established more than 100 years ago, tissue clearing still rapidly evolving field of research. There are currently many published protocols to choose from, each performs better or worse across range key evaluation factors (e.g., speed, cost, stability, fluorescence quenching). Additionally, often optimized for specific experimental contexts, applying an existing new problem can require lengthy period adaptation by...